Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4265 and GFF4266 are separated by 40 nucleotides GFF4266 and GFF4267 are separated by 103 nucleotides
GFF4265 - 2-methylcitrate dehydratase (EC 4.2.1.79), at 50,408 to 51,859
GFF4265
GFF4266 - Propionate--CoA ligase (EC 6.2.1.17), at 51,900 to 53,786
GFF4266
GFF4267 - Porphobilinogen synthase (EC 4.2.1.24), at 53,890 to 54,864
GFF4267
Position (kb)
51
52
53
54 Strain fitness (log2 ratio)
-1
0
1
2 at 51.234 kb on - strand, within GFF4265 at 51.455 kb on + strand, within GFF4265 at 51.455 kb on + strand, within GFF4265 at 51.456 kb on - strand, within GFF4265 at 51.613 kb on + strand, within GFF4265 at 51.614 kb on - strand, within GFF4265 at 51.641 kb on - strand, within GFF4265 at 51.846 kb on + strand at 51.872 kb on + strand at 52.052 kb on + strand at 52.057 kb on + strand at 52.058 kb on - strand at 52.058 kb on - strand at 52.058 kb on - strand at 52.064 kb on + strand at 52.064 kb on + strand at 52.065 kb on - strand at 52.239 kb on + strand, within GFF4266 at 52.239 kb on + strand, within GFF4266 at 52.242 kb on - strand, within GFF4266 at 52.442 kb on + strand, within GFF4266 at 52.614 kb on + strand, within GFF4266 at 52.615 kb on - strand, within GFF4266 at 52.615 kb on - strand, within GFF4266 at 52.770 kb on + strand, within GFF4266 at 52.770 kb on + strand, within GFF4266 at 52.978 kb on - strand, within GFF4266 at 53.148 kb on + strand, within GFF4266 at 53.149 kb on - strand, within GFF4266 at 53.156 kb on + strand, within GFF4266 at 53.167 kb on + strand, within GFF4266 at 53.167 kb on + strand, within GFF4266 at 53.168 kb on - strand, within GFF4266 at 53.250 kb on - strand, within GFF4266 at 53.258 kb on + strand, within GFF4266 at 53.362 kb on - strand, within GFF4266 at 53.362 kb on - strand, within GFF4266 at 53.394 kb on - strand, within GFF4266 at 53.429 kb on - strand, within GFF4266 at 53.507 kb on - strand, within GFF4266
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 51,234 - GFF4265 0.57 +2.1 51,455 + GFF4265 0.72 -0.1 51,455 + GFF4265 0.72 -0.7 51,456 - GFF4265 0.72 +0.2 51,613 + GFF4265 0.83 -0.6 51,614 - GFF4265 0.83 +0.2 51,641 - GFF4265 0.85 +0.1 51,846 + +0.6 51,872 + -0.1 52,052 + +0.2 52,057 + +0.1 52,058 - -0.8 52,058 - -0.8 52,058 - +0.1 52,064 + +0.4 52,064 + +0.3 52,065 - -0.5 52,239 + GFF4266 0.18 -0.1 52,239 + GFF4266 0.18 -0.6 52,242 - GFF4266 0.18 -0.1 52,442 + GFF4266 0.29 +0.1 52,614 + GFF4266 0.38 -0.0 52,615 - GFF4266 0.38 +1.3 52,615 - GFF4266 0.38 +0.6 52,770 + GFF4266 0.46 -1.1 52,770 + GFF4266 0.46 +0.6 52,978 - GFF4266 0.57 +0.3 53,148 + GFF4266 0.66 -0.2 53,149 - GFF4266 0.66 +0.2 53,156 + GFF4266 0.67 +1.0 53,167 + GFF4266 0.67 +0.3 53,167 + GFF4266 0.67 +0.3 53,168 - GFF4266 0.67 -0.6 53,250 - GFF4266 0.72 +0.2 53,258 + GFF4266 0.72 -0.1 53,362 - GFF4266 0.77 +0.1 53,362 - GFF4266 0.77 +1.1 53,394 - GFF4266 0.79 +0.5 53,429 - GFF4266 0.81 -0.0 53,507 - GFF4266 0.85 +1.3
Or see this region's nucleotide sequence