Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4023 and GFF4024 are separated by 185 nucleotides GFF4024 and GFF4025 are separated by 39 nucleotides GFF4025 and GFF4026 are separated by 71 nucleotides GFF4026 and GFF4027 overlap by 1 nucleotides GFF4027 and GFF4028 are separated by 14 nucleotides
GFF4023 - Putative transport system permease protein, at 289,846 to 291,051
GFF4023
GFF4024 - FIG00640199: hypothetical protein, at 291,237 to 291,662
GFF4024
GFF4025 - Cytoplasmic alpha-amylase (EC 3.2.1.1), at 291,702 to 293,186
GFF4025
GFF4026 - Flagellar biosynthesis protein FliT, at 293,258 to 293,626
GFF4026
GFF4027 - Flagellar biosynthesis protein FliS, at 293,626 to 294,033
GFF4027
GFF4028 - Flagellar hook-associated protein FliD, at 294,048 to 295,451
GFF4028
Position (kb) 291
292
293
294 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 290.750 kb on - strand, within GFF4023 at 290.855 kb on + strand, within GFF4023 at 290.856 kb on - strand, within GFF4023 at 290.960 kb on + strand at 291.123 kb on - strand at 291.123 kb on - strand at 291.129 kb on - strand at 291.496 kb on + strand, within GFF4024 at 291.525 kb on + strand, within GFF4024 at 291.830 kb on - strand at 291.935 kb on + strand, within GFF4025 at 291.960 kb on + strand, within GFF4025 at 291.961 kb on - strand, within GFF4025 at 292.191 kb on - strand, within GFF4025 at 292.270 kb on + strand, within GFF4025 at 292.358 kb on - strand, within GFF4025 at 292.432 kb on - strand, within GFF4025 at 292.452 kb on - strand, within GFF4025 at 292.535 kb on - strand, within GFF4025 at 292.602 kb on - strand, within GFF4025 at 292.646 kb on - strand, within GFF4025 at 292.717 kb on + strand, within GFF4025 at 292.718 kb on - strand, within GFF4025 at 292.718 kb on - strand, within GFF4025 at 292.738 kb on + strand, within GFF4025 at 293.026 kb on - strand, within GFF4025 at 293.356 kb on + strand, within GFF4026 at 293.356 kb on + strand, within GFF4026 at 293.357 kb on - strand, within GFF4026 at 293.588 kb on - strand, within GFF4026 at 293.683 kb on - strand, within GFF4027 at 293.769 kb on - strand, within GFF4027 at 294.063 kb on - strand at 294.090 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 290,750 - GFF4023 0.75 -0.1 290,855 + GFF4023 0.84 -0.5 290,856 - GFF4023 0.84 +0.4 290,960 + -0.1 291,123 - +0.9 291,123 - -0.3 291,129 - -1.1 291,496 + GFF4024 0.61 +0.2 291,525 + GFF4024 0.68 -0.2 291,830 - +0.6 291,935 + GFF4025 0.16 +0.4 291,960 + GFF4025 0.17 -0.9 291,961 - GFF4025 0.17 -0.3 292,191 - GFF4025 0.33 +0.6 292,270 + GFF4025 0.38 -0.1 292,358 - GFF4025 0.44 +0.3 292,432 - GFF4025 0.49 +0.9 292,452 - GFF4025 0.51 +0.0 292,535 - GFF4025 0.56 -0.0 292,602 - GFF4025 0.61 +0.7 292,646 - GFF4025 0.64 +0.6 292,717 + GFF4025 0.68 +0.1 292,718 - GFF4025 0.68 +0.0 292,718 - GFF4025 0.68 -0.1 292,738 + GFF4025 0.70 +0.1 293,026 - GFF4025 0.89 -1.1 293,356 + GFF4026 0.27 -0.8 293,356 + GFF4026 0.27 +0.6 293,357 - GFF4026 0.27 -0.4 293,588 - GFF4026 0.89 -2.2 293,683 - GFF4027 0.14 +3.0 293,769 - GFF4027 0.35 +0.7 294,063 - -0.7 294,090 - +0.2
Or see this region's nucleotide sequence