Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4007

Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4006 and GFF4007 are separated by 8 nucleotidesGFF4007 and GFF4008 are separated by 9 nucleotidesGFF4008 and GFF4009 overlap by 1 nucleotides GFF4006 - Flagellar biosynthesis protein FliR, at 277,708 to 278,502 GFF4006 GFF4007 - Flagellar biosynthesis protein FliQ, at 278,511 to 278,780 GFF4007 GFF4008 - Flagellar biosynthesis protein FliP, at 278,790 to 279,527 GFF4008 GFF4009 - Flagellar biosynthesis protein FliQ, at 279,527 to 279,847 GFF4009 Position (kb) 278 279Strain fitness (log2 ratio) -1 0 1 2at 278.099 kb on - strand, within GFF4006at 278.113 kb on - strand, within GFF4006at 278.118 kb on + strand, within GFF4006at 278.139 kb on + strand, within GFF4006at 278.457 kb on - strandat 278.472 kb on - strandat 278.473 kb on + strandat 278.522 kb on + strandat 278.525 kb on - strandat 278.554 kb on + strand, within GFF4007at 278.594 kb on + strand, within GFF4007at 278.707 kb on - strand, within GFF4007at 279.262 kb on - strand, within GFF4008at 279.513 kb on + strandat 279.513 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
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278,099 - GFF4006 0.49 +0.3
278,113 - GFF4006 0.51 +1.2
278,118 + GFF4006 0.52 +1.3
278,139 + GFF4006 0.54 +2.5
278,457 - -0.4
278,472 - +1.8
278,473 + +0.8
278,522 + +1.4
278,525 - +1.0
278,554 + GFF4007 0.16 -0.3
278,594 + GFF4007 0.31 +1.7
278,707 - GFF4007 0.73 +1.2
279,262 - GFF4008 0.64 +1.6
279,513 + +1.2
279,513 + +1.8

Or see this region's nucleotide sequence