Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3949 and GFF3950 are separated by 597 nucleotides GFF3950 and GFF3951 overlap by 4 nucleotides GFF3951 and GFF3952 are separated by 10 nucleotides
GFF3949 - Propanediol utilization transcriptional activator, at 229,543 to 230,454
GFF3949
GFF3950 - Cobyrinic acid A,C-diamide synthase, at 231,052 to 232,431
GFF3950
GFF3951 - Adenosylcobinamide-phosphate synthase (EC 6.3.1.10), at 232,428 to 233,387
GFF3951
GFF3952 - Cobalt-precorrin-8x methylmutase (EC 5.4.1.2), at 233,398 to 234,030
GFF3952
Position (kb) 231
232
233 Strain fitness (log2 ratio) -2
-1
0
1
2 at 230.118 kb on + strand, within GFF3949 at 230.210 kb on - strand, within GFF3949 at 230.578 kb on + strand at 230.749 kb on + strand at 230.752 kb on + strand at 230.752 kb on + strand at 230.788 kb on + strand at 230.988 kb on - strand at 231.020 kb on - strand at 231.208 kb on - strand, within GFF3950 at 231.337 kb on + strand, within GFF3950 at 231.338 kb on - strand, within GFF3950 at 231.338 kb on - strand, within GFF3950 at 231.338 kb on - strand, within GFF3950 at 231.338 kb on - strand, within GFF3950 at 231.365 kb on - strand, within GFF3950 at 231.590 kb on - strand, within GFF3950 at 231.834 kb on + strand, within GFF3950 at 231.841 kb on + strand, within GFF3950 at 231.965 kb on + strand, within GFF3950 at 231.968 kb on - strand, within GFF3950 at 232.066 kb on + strand, within GFF3950 at 232.169 kb on - strand, within GFF3950 at 232.244 kb on - strand, within GFF3950 at 232.287 kb on + strand, within GFF3950 at 232.427 kb on + strand at 232.594 kb on + strand, within GFF3951 at 233.046 kb on + strand, within GFF3951 at 233.145 kb on + strand, within GFF3951 at 233.148 kb on - strand, within GFF3951 at 233.156 kb on + strand, within GFF3951 at 233.225 kb on + strand, within GFF3951 at 233.225 kb on + strand, within GFF3951 at 233.397 kb on + strand at 233.398 kb on - strand at 233.398 kb on - strand at 233.398 kb on - strand at 233.404 kb on + strand at 233.404 kb on + strand at 233.405 kb on - strand at 233.407 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 230,118 + GFF3949 0.63 +1.5 230,210 - GFF3949 0.73 -2.2 230,578 + -1.4 230,749 + -0.2 230,752 + +0.1 230,752 + -0.2 230,788 + -0.4 230,988 - -0.8 231,020 - +0.6 231,208 - GFF3950 0.11 +0.2 231,337 + GFF3950 0.21 -0.8 231,338 - GFF3950 0.21 -0.6 231,338 - GFF3950 0.21 -0.0 231,338 - GFF3950 0.21 +0.4 231,338 - GFF3950 0.21 -0.5 231,365 - GFF3950 0.23 -0.3 231,590 - GFF3950 0.39 -0.3 231,834 + GFF3950 0.57 +0.3 231,841 + GFF3950 0.57 -0.8 231,965 + GFF3950 0.66 -0.2 231,968 - GFF3950 0.66 -0.8 232,066 + GFF3950 0.73 -0.0 232,169 - GFF3950 0.81 +0.2 232,244 - GFF3950 0.86 -0.8 232,287 + GFF3950 0.89 -0.6 232,427 + -0.0 232,594 + GFF3951 0.17 -1.1 233,046 + GFF3951 0.64 +1.1 233,145 + GFF3951 0.75 -0.9 233,148 - GFF3951 0.75 +0.1 233,156 + GFF3951 0.76 +0.0 233,225 + GFF3951 0.83 +2.5 233,225 + GFF3951 0.83 -0.6 233,397 + +0.6 233,398 - +0.1 233,398 - -0.1 233,398 - +1.0 233,404 + -0.2 233,404 + -0.2 233,405 - +0.2 233,407 - -0.7
Or see this region's nucleotide sequence