Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3884 and GFF3885 are separated by 10 nucleotides GFF3885 and GFF3886 are separated by 177 nucleotides
GFF3884 - Colanic acid biosynthesis glycosyl transferase WcaL, at 162,191 to 163,411
GFF3884
GFF3885 - Colanic acid biosynthesis protein wcaM, at 163,422 to 164,825
GFF3885
GFF3886 - UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9), at 165,003 to 165,896
GFF3886
Position (kb) 163
164
165 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 162.422 kb on - strand, within GFF3884 at 162.557 kb on - strand, within GFF3884 at 162.557 kb on - strand, within GFF3884 at 162.653 kb on - strand, within GFF3884 at 162.658 kb on + strand, within GFF3884 at 163.139 kb on - strand, within GFF3884 at 163.185 kb on + strand, within GFF3884 at 163.189 kb on + strand, within GFF3884 at 163.409 kb on + strand at 163.421 kb on + strand at 163.809 kb on - strand, within GFF3885 at 163.996 kb on + strand, within GFF3885 at 164.075 kb on + strand, within GFF3885 at 164.082 kb on - strand, within GFF3885 at 164.134 kb on - strand, within GFF3885 at 164.146 kb on - strand, within GFF3885 at 164.410 kb on - strand, within GFF3885 at 164.437 kb on + strand, within GFF3885 at 164.472 kb on + strand, within GFF3885 at 164.666 kb on - strand, within GFF3885 at 164.666 kb on - strand, within GFF3885 at 164.765 kb on + strand at 164.869 kb on - strand at 164.898 kb on - strand at 165.023 kb on + strand at 165.050 kb on + strand at 165.050 kb on + strand at 165.050 kb on + strand at 165.050 kb on + strand at 165.051 kb on - strand at 165.051 kb on - strand at 165.051 kb on - strand at 165.117 kb on + strand, within GFF3886 at 165.118 kb on - strand, within GFF3886 at 165.118 kb on - strand, within GFF3886 at 165.213 kb on + strand, within GFF3886 at 165.213 kb on + strand, within GFF3886 at 165.275 kb on + strand, within GFF3886 at 165.341 kb on + strand, within GFF3886 at 165.341 kb on + strand, within GFF3886 at 165.342 kb on - strand, within GFF3886 at 165.435 kb on + strand, within GFF3886 at 165.435 kb on + strand, within GFF3886 at 165.436 kb on - strand, within GFF3886 at 165.437 kb on + strand, within GFF3886 at 165.783 kb on + strand, within GFF3886 at 165.784 kb on - strand, within GFF3886
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 162,422 - GFF3884 0.19 -1.5 162,557 - GFF3884 0.30 -0.4 162,557 - GFF3884 0.30 +0.3 162,653 - GFF3884 0.38 +1.8 162,658 + GFF3884 0.38 +1.6 163,139 - GFF3884 0.78 +0.1 163,185 + GFF3884 0.81 +2.9 163,189 + GFF3884 0.82 +0.9 163,409 + +0.9 163,421 + -0.1 163,809 - GFF3885 0.28 -0.0 163,996 + GFF3885 0.41 +1.4 164,075 + GFF3885 0.47 +0.1 164,082 - GFF3885 0.47 -0.4 164,134 - GFF3885 0.51 -0.8 164,146 - GFF3885 0.52 -0.8 164,410 - GFF3885 0.70 +1.9 164,437 + GFF3885 0.72 +1.6 164,472 + GFF3885 0.75 +1.1 164,666 - GFF3885 0.89 +0.2 164,666 - GFF3885 0.89 +0.8 164,765 + +2.7 164,869 - +1.6 164,898 - +0.6 165,023 + -1.9 165,050 + -1.9 165,050 + -1.1 165,050 + -1.8 165,050 + -3.2 165,051 - -1.8 165,051 - -0.3 165,051 - -2.5 165,117 + GFF3886 0.13 -3.1 165,118 - GFF3886 0.13 -1.4 165,118 - GFF3886 0.13 -3.3 165,213 + GFF3886 0.23 -1.9 165,213 + GFF3886 0.23 -1.6 165,275 + GFF3886 0.30 -2.3 165,341 + GFF3886 0.38 -0.5 165,341 + GFF3886 0.38 -1.5 165,342 - GFF3886 0.38 -3.6 165,435 + GFF3886 0.48 -2.7 165,435 + GFF3886 0.48 -1.9 165,436 - GFF3886 0.48 -1.4 165,437 + GFF3886 0.49 -3.0 165,783 + GFF3886 0.87 -2.0 165,784 - GFF3886 0.87 -2.4
Or see this region's nucleotide sequence