Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3849 and GFF3850 are separated by 268 nucleotides GFF3850 and GFF3851 overlap by 4 nucleotides GFF3851 and GFF3852 overlap by 1 nucleotides
GFF3849 - FIG074102: hypothetical protein, at 118,122 to 119,324
GFF3849
GFF3850 - Response regulator BaeR, at 119,593 to 120,315
GFF3850
GFF3851 - Sensory histidine kinase BaeS, at 120,312 to 121,715
GFF3851
GFF3852 - Multidrug transporter MdtD, at 121,715 to 123,127
GFF3852
Position (kb)
120
121
122 Strain fitness (log2 ratio)
-1
0
1
2 at 119.588 kb on - strand at 119.653 kb on - strand at 119.703 kb on + strand, within GFF3850 at 119.719 kb on - strand, within GFF3850 at 120.101 kb on - strand, within GFF3850 at 120.316 kb on - strand at 120.316 kb on - strand at 120.345 kb on + strand at 120.346 kb on - strand at 120.352 kb on - strand at 120.430 kb on - strand at 120.510 kb on + strand, within GFF3851 at 120.639 kb on - strand, within GFF3851 at 120.683 kb on + strand, within GFF3851 at 120.684 kb on - strand, within GFF3851 at 120.684 kb on - strand, within GFF3851 at 120.684 kb on - strand, within GFF3851 at 120.706 kb on - strand, within GFF3851 at 120.873 kb on + strand, within GFF3851 at 121.591 kb on + strand at 121.759 kb on + strand at 121.759 kb on + strand at 121.801 kb on + strand at 121.805 kb on - strand at 121.805 kb on - strand at 121.813 kb on + strand at 121.814 kb on - strand at 121.814 kb on - strand at 121.814 kb on - strand at 121.817 kb on + strand at 121.936 kb on + strand, within GFF3852 at 122.067 kb on + strand, within GFF3852 at 122.068 kb on - strand, within GFF3852 at 122.168 kb on + strand, within GFF3852 at 122.169 kb on - strand, within GFF3852 at 122.558 kb on + strand, within GFF3852 at 122.559 kb on - strand, within GFF3852 at 122.566 kb on - strand, within GFF3852 at 122.593 kb on + strand, within GFF3852
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 119,588 - +0.0 119,653 - -0.5 119,703 + GFF3850 0.15 -0.7 119,719 - GFF3850 0.17 +0.3 120,101 - GFF3850 0.70 +0.6 120,316 - -0.5 120,316 - -0.7 120,345 + -0.4 120,346 - +0.3 120,352 - -0.4 120,430 - +0.2 120,510 + GFF3851 0.14 +0.9 120,639 - GFF3851 0.23 +0.0 120,683 + GFF3851 0.26 +1.1 120,684 - GFF3851 0.26 -0.5 120,684 - GFF3851 0.26 -0.9 120,684 - GFF3851 0.26 +2.2 120,706 - GFF3851 0.28 +0.1 120,873 + GFF3851 0.40 +0.0 121,591 + -0.1 121,759 + -0.1 121,759 + +0.0 121,801 + +1.7 121,805 - +0.3 121,805 - -0.5 121,813 + +0.3 121,814 - +0.5 121,814 - -0.3 121,814 - +0.5 121,817 + -0.3 121,936 + GFF3852 0.16 +0.3 122,067 + GFF3852 0.25 +0.5 122,068 - GFF3852 0.25 -0.8 122,168 + GFF3852 0.32 +0.6 122,169 - GFF3852 0.32 +0.7 122,558 + GFF3852 0.60 -0.2 122,559 - GFF3852 0.60 +1.5 122,566 - GFF3852 0.60 -1.6 122,593 + GFF3852 0.62 -0.4
Or see this region's nucleotide sequence