Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3747 and GFF3748 are separated by 31 nucleotides GFF3748 and GFF3749 are separated by 179 nucleotides GFF3749 and GFF3750 are separated by 84 nucleotides GFF3750 and GFF3751 are separated by 108 nucleotides
GFF3747 - FIG001881: hydrolase of alkaline phosphatase superfamily, at 14,171 to 15,931
GFF3747
GFF3748 - FIG002927: hypothetical protein, at 15,963 to 16,190
GFF3748
GFF3749 - Nucleoid-associated protein NdpA, at 16,370 to 17,377
GFF3749
GFF3750 - Putative inner membrane protein, at 17,462 to 18,025
GFF3750
GFF3751 - LSU ribosomal protein L25p, at 18,134 to 18,418
GFF3751
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-1
0
1 at 16.074 kb on - strand, within GFF3748 at 16.170 kb on - strand at 16.226 kb on - strand at 16.229 kb on - strand at 16.509 kb on - strand, within GFF3749 at 16.677 kb on + strand, within GFF3749 at 16.678 kb on - strand, within GFF3749 at 16.775 kb on - strand, within GFF3749 at 17.222 kb on + strand, within GFF3749 at 17.384 kb on - strand at 17.541 kb on - strand, within GFF3750 at 17.542 kb on + strand, within GFF3750 at 17.547 kb on - strand, within GFF3750 at 17.646 kb on + strand, within GFF3750 at 17.651 kb on + strand, within GFF3750 at 17.763 kb on + strand, within GFF3750 at 17.763 kb on + strand, within GFF3750 at 17.763 kb on + strand, within GFF3750 at 17.833 kb on - strand, within GFF3750 at 17.880 kb on + strand, within GFF3750 at 17.886 kb on + strand, within GFF3750 at 17.978 kb on - strand at 18.024 kb on - strand at 18.024 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 16,074 - GFF3748 0.49 -1.0 16,170 - +1.0 16,226 - -0.1 16,229 - +0.2 16,509 - GFF3749 0.14 -1.1 16,677 + GFF3749 0.30 -0.7 16,678 - GFF3749 0.31 -1.2 16,775 - GFF3749 0.40 -0.7 17,222 + GFF3749 0.85 -1.1 17,384 - -0.4 17,541 - GFF3750 0.14 -0.2 17,542 + GFF3750 0.14 -0.5 17,547 - GFF3750 0.15 -0.1 17,646 + GFF3750 0.33 -0.1 17,651 + GFF3750 0.34 -0.4 17,763 + GFF3750 0.53 -0.6 17,763 + GFF3750 0.53 +0.2 17,763 + GFF3750 0.53 +0.4 17,833 - GFF3750 0.66 -0.1 17,880 + GFF3750 0.74 -0.4 17,886 + GFF3750 0.75 -0.3 17,978 - -0.2 18,024 - +0.4 18,024 - -0.2
Or see this region's nucleotide sequence