Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3510 and GFF3511 are separated by 136 nucleotides GFF3511 and GFF3512 are separated by 137 nucleotides GFF3512 and GFF3513 are separated by 148 nucleotides GFF3513 and GFF3514 are separated by 42 nucleotides
GFF3510 - L-cystine uptake protein TcyP, at 262,128 to 263,519
GFF3510
GFF3511 - Membrane-bound metal-dependent hydrolase YdjM, induced during SOS response, at 263,656 to 264,255
GFF3511
GFF3512 - 2-deoxyglucose-6-phosphate hydrolase YniC, at 264,393 to 265,061
GFF3512
GFF3513 - Putative membrane protein, at 265,210 to 265,746
GFF3513
GFF3514 - Ribulosamine/erythrulosamine 3-kinase potentially involved in protein deglycation, at 265,789 to 266,649
GFF3514
Position (kb) 264
265
266 Strain fitness (log2 ratio) -2
-1
0
1 at 263.448 kb on - strand at 263.506 kb on - strand at 263.703 kb on + strand at 263.706 kb on + strand at 263.728 kb on + strand, within GFF3511 at 263.978 kb on + strand, within GFF3511 at 264.601 kb on + strand, within GFF3512 at 264.825 kb on - strand, within GFF3512 at 264.945 kb on - strand, within GFF3512 at 264.949 kb on - strand, within GFF3512 at 264.949 kb on - strand, within GFF3512 at 264.955 kb on - strand, within GFF3512 at 265.127 kb on - strand at 265.128 kb on + strand at 265.129 kb on - strand at 265.129 kb on - strand at 265.216 kb on + strand at 265.217 kb on - strand at 265.318 kb on + strand, within GFF3513 at 265.318 kb on + strand, within GFF3513 at 265.318 kb on + strand, within GFF3513 at 265.376 kb on + strand, within GFF3513 at 265.377 kb on - strand, within GFF3513 at 265.625 kb on + strand, within GFF3513 at 265.636 kb on - strand, within GFF3513 at 265.791 kb on - strand at 265.883 kb on + strand, within GFF3514 at 265.943 kb on + strand, within GFF3514 at 266.029 kb on - strand, within GFF3514 at 266.030 kb on + strand, within GFF3514 at 266.030 kb on + strand, within GFF3514
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 263,448 - +1.2 263,506 - -0.5 263,703 + +0.4 263,706 + +0.4 263,728 + GFF3511 0.12 +0.3 263,978 + GFF3511 0.54 -1.4 264,601 + GFF3512 0.31 -0.6 264,825 - GFF3512 0.65 +0.0 264,945 - GFF3512 0.83 +0.1 264,949 - GFF3512 0.83 -0.5 264,949 - GFF3512 0.83 +1.0 264,955 - GFF3512 0.84 +0.7 265,127 - +0.5 265,128 + +0.9 265,129 - -0.0 265,129 - +0.0 265,216 + -0.3 265,217 - -0.6 265,318 + GFF3513 0.20 +0.4 265,318 + GFF3513 0.20 -0.9 265,318 + GFF3513 0.20 -0.8 265,376 + GFF3513 0.31 -0.7 265,377 - GFF3513 0.31 -1.7 265,625 + GFF3513 0.77 -0.8 265,636 - GFF3513 0.79 +0.7 265,791 - -0.1 265,883 + GFF3514 0.11 +0.2 265,943 + GFF3514 0.18 +0.4 266,029 - GFF3514 0.28 -0.2 266,030 + GFF3514 0.28 -0.1 266,030 + GFF3514 0.28 -2.0
Or see this region's nucleotide sequence