Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3235 and GFF3236 are separated by 207 nucleotides GFF3236 and GFF3237 are separated by 165 nucleotides GFF3237 and GFF3238 overlap by 4 nucleotides
GFF3235 - Membrane alanine aminopeptidase N (EC 3.4.11.2), at 6,007 to 8,619
GFF3235
GFF3236 - Dihydroorotate dehydrogenase (EC 1.3.3.1), at 8,827 to 9,837
GFF3236
GFF3237 - hypothetical protein, at 10,003 to 10,545
GFF3237
GFF3238 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1, at 10,542 to 11,651
GFF3238
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-2
-1
0
1 at 7.984 kb on + strand, within GFF3235 at 7.984 kb on + strand, within GFF3235 at 8.094 kb on + strand, within GFF3235 at 8.098 kb on - strand, within GFF3235 at 8.134 kb on - strand, within GFF3235 at 8.217 kb on + strand, within GFF3235 at 8.332 kb on - strand, within GFF3235 at 8.512 kb on + strand at 8.520 kb on + strand at 8.605 kb on - strand at 8.753 kb on - strand at 8.830 kb on + strand at 8.831 kb on - strand at 8.847 kb on + strand at 8.914 kb on - strand at 8.982 kb on + strand, within GFF3236 at 9.048 kb on - strand, within GFF3236 at 9.171 kb on + strand, within GFF3236 at 9.229 kb on - strand, within GFF3236 at 9.328 kb on + strand, within GFF3236 at 9.408 kb on - strand, within GFF3236 at 9.453 kb on + strand, within GFF3236 at 9.454 kb on - strand, within GFF3236 at 9.457 kb on + strand, within GFF3236 at 9.457 kb on + strand, within GFF3236 at 9.645 kb on - strand, within GFF3236 at 10.002 kb on - strand at 10.083 kb on + strand, within GFF3237 at 10.442 kb on + strand, within GFF3237 at 10.729 kb on - strand, within GFF3238 at 10.780 kb on - strand, within GFF3238 at 10.837 kb on + strand, within GFF3238
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 7,984 + GFF3235 0.76 +0.0 7,984 + GFF3235 0.76 +0.1 8,094 + GFF3235 0.80 -0.2 8,098 - GFF3235 0.80 -0.2 8,134 - GFF3235 0.81 -1.0 8,217 + GFF3235 0.85 +0.3 8,332 - GFF3235 0.89 -0.5 8,512 + -1.4 8,520 + -0.3 8,605 - -1.5 8,753 - +1.3 8,830 + -1.0 8,831 - -2.3 8,847 + -1.0 8,914 - +0.2 8,982 + GFF3236 0.15 -0.6 9,048 - GFF3236 0.22 -0.8 9,171 + GFF3236 0.34 +0.7 9,229 - GFF3236 0.40 -0.6 9,328 + GFF3236 0.50 -1.1 9,408 - GFF3236 0.57 -2.0 9,453 + GFF3236 0.62 -2.3 9,454 - GFF3236 0.62 +0.0 9,457 + GFF3236 0.62 -1.1 9,457 + GFF3236 0.62 -0.7 9,645 - GFF3236 0.81 -0.4 10,002 - +0.5 10,083 + GFF3237 0.15 -0.5 10,442 + GFF3237 0.81 +0.0 10,729 - GFF3238 0.17 -0.5 10,780 - GFF3238 0.21 +0.5 10,837 + GFF3238 0.27 -0.3
Or see this region's nucleotide sequence