Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3141 and GFF3142 are separated by 31 nucleotides GFF3142 and GFF3143 are separated by 4 nucleotides GFF3143 and GFF3144 are separated by 3 nucleotides GFF3144 and GFF3145 are separated by 27 nucleotides GFF3145 and GFF3146 overlap by 1 nucleotides
GFF3141 - Modulator of drug activity B, at 148,045 to 148,626
GFF3141
GFF3142 - FIG00896075: hypothetical protein, at 148,658 to 148,972
GFF3142
GFF3143 - hypothetical protein, at 148,977 to 149,093
GFF3143
GFF3144 - Topoisomerase IV subunit B (EC 5.99.1.-), at 149,097 to 150,989
GFF3144
GFF3145 - Putative esterase, FIGfam005057, at 151,017 to 151,598
GFF3145
GFF3146 - '3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17)' transl_table=11, at 151,598 to 152,425
GFF3146
Position (kb) 149
150
151 Strain fitness (log2 ratio) -1
0
1
2 at 148.186 kb on + strand, within GFF3141 at 148.288 kb on - strand, within GFF3141 at 148.325 kb on - strand, within GFF3141 at 148.401 kb on + strand, within GFF3141 at 148.470 kb on - strand, within GFF3141 at 148.476 kb on - strand, within GFF3141 at 148.584 kb on + strand at 148.653 kb on + strand at 148.654 kb on - strand at 148.754 kb on - strand, within GFF3142 at 148.898 kb on - strand, within GFF3142 at 148.978 kb on + strand at 149.079 kb on + strand, within GFF3143 at 149.079 kb on + strand, within GFF3143 at 151.127 kb on - strand, within GFF3145 at 151.127 kb on - strand, within GFF3145 at 151.255 kb on + strand, within GFF3145 at 151.256 kb on - strand, within GFF3145 at 151.256 kb on - strand, within GFF3145 at 151.256 kb on - strand, within GFF3145 at 151.352 kb on + strand, within GFF3145 at 151.715 kb on - strand, within GFF3146 at 151.816 kb on + strand, within GFF3146
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 148,186 + GFF3141 0.24 +0.1 148,288 - GFF3141 0.42 -0.1 148,325 - GFF3141 0.48 +0.2 148,401 + GFF3141 0.61 +0.0 148,470 - GFF3141 0.73 -0.2 148,476 - GFF3141 0.74 -0.3 148,584 + -0.2 148,653 + -0.5 148,654 - +0.6 148,754 - GFF3142 0.30 -0.4 148,898 - GFF3142 0.76 +0.7 148,978 + -0.0 149,079 + GFF3143 0.87 +0.1 149,079 + GFF3143 0.87 -0.4 151,127 - GFF3145 0.19 -0.4 151,127 - GFF3145 0.19 +0.3 151,255 + GFF3145 0.41 -0.2 151,256 - GFF3145 0.41 +2.7 151,256 - GFF3145 0.41 -0.4 151,256 - GFF3145 0.41 -0.1 151,352 + GFF3145 0.58 -0.9 151,715 - GFF3146 0.14 -1.4 151,816 + GFF3146 0.26 -0.6
Or see this region's nucleotide sequence