Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3003 and GFF3004 are separated by 162 nucleotides GFF3004 and GFF3005 are separated by 25 nucleotides
GFF3003 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 17,271 to 20,144
GFF3003
GFF3004 - Glycine cleavage system H protein, at 20,307 to 20,696
GFF3004
GFF3005 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 20,722 to 21,816
GFF3005
Position (kb) 20
21 Strain fitness (log2 ratio) -1
0
1 at 19.486 kb on - strand, within GFF3003 at 19.494 kb on + strand, within GFF3003 at 19.810 kb on + strand, within GFF3003 at 19.810 kb on + strand, within GFF3003 at 19.819 kb on + strand, within GFF3003 at 19.879 kb on + strand at 19.879 kb on + strand at 19.894 kb on + strand at 19.895 kb on - strand at 19.913 kb on + strand at 20.624 kb on - strand, within GFF3004 at 20.624 kb on - strand, within GFF3004 at 20.624 kb on - strand, within GFF3004 at 20.643 kb on - strand, within GFF3004 at 20.702 kb on - strand at 20.849 kb on + strand, within GFF3005 at 20.938 kb on - strand, within GFF3005 at 20.938 kb on - strand, within GFF3005 at 21.025 kb on - strand, within GFF3005 at 21.087 kb on + strand, within GFF3005 at 21.088 kb on - strand, within GFF3005 at 21.234 kb on - strand, within GFF3005 at 21.306 kb on + strand, within GFF3005 at 21.306 kb on + strand, within GFF3005 at 21.306 kb on + strand, within GFF3005 at 21.307 kb on - strand, within GFF3005 at 21.307 kb on - strand, within GFF3005 at 21.307 kb on - strand, within GFF3005 at 21.336 kb on + strand, within GFF3005 at 21.477 kb on + strand, within GFF3005 at 21.509 kb on + strand, within GFF3005 at 21.512 kb on + strand, within GFF3005 at 21.587 kb on - strand, within GFF3005 at 21.614 kb on + strand, within GFF3005 at 21.619 kb on + strand, within GFF3005
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 19,486 - GFF3003 0.77 -0.2 19,494 + GFF3003 0.77 -1.2 19,810 + GFF3003 0.88 -0.1 19,810 + GFF3003 0.88 +0.0 19,819 + GFF3003 0.89 +0.7 19,879 + -0.9 19,879 + -0.1 19,894 + +0.2 19,895 - -0.0 19,913 + +0.2 20,624 - GFF3004 0.81 -0.1 20,624 - GFF3004 0.81 -0.3 20,624 - GFF3004 0.81 +0.9 20,643 - GFF3004 0.86 +0.4 20,702 - +0.3 20,849 + GFF3005 0.12 +0.1 20,938 - GFF3005 0.20 -0.0 20,938 - GFF3005 0.20 +0.4 21,025 - GFF3005 0.28 +0.5 21,087 + GFF3005 0.33 -0.3 21,088 - GFF3005 0.33 +1.1 21,234 - GFF3005 0.47 -0.7 21,306 + GFF3005 0.53 +0.4 21,306 + GFF3005 0.53 -1.5 21,306 + GFF3005 0.53 +1.2 21,307 - GFF3005 0.53 -0.1 21,307 - GFF3005 0.53 +1.4 21,307 - GFF3005 0.53 +0.1 21,336 + GFF3005 0.56 +0.7 21,477 + GFF3005 0.69 -1.0 21,509 + GFF3005 0.72 +0.0 21,512 + GFF3005 0.72 +0.7 21,587 - GFF3005 0.79 +0.5 21,614 + GFF3005 0.81 -0.0 21,619 + GFF3005 0.82 -1.5
Or see this region's nucleotide sequence