Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2795 and GFF2796 are separated by 17 nucleotides GFF2796 and GFF2797 are separated by 79 nucleotides GFF2797 and GFF2798 are separated by 62 nucleotides GFF2798 and GFF2799 are separated by 168 nucleotides
GFF2795 - NAD-specific glutamate dehydrogenase (EC 1.4.1.2); NADP-specific glutamate dehydrogenase (EC 1.4.1.4), at 318,851 to 320,125
GFF2795
GFF2796 - hypothetical protein, at 320,143 to 320,259
GFF2796
GFF2797 - Trehalase (EC 3.2.1.28); Periplasmic trehalase precursor (EC 3.2.1.28), at 320,339 to 322,051
GFF2797
GFF2798 - UPF0410 protein YmgE, at 322,114 to 322,368
GFF2798
GFF2799 - Inner membrane protein, at 322,537 to 323,271
GFF2799
Position (kb) 320
321
322
323 Strain fitness (log2 ratio) -2
-1
0
1
2 at 319.352 kb on + strand, within GFF2795 at 319.352 kb on + strand, within GFF2795 at 319.403 kb on - strand, within GFF2795 at 319.403 kb on - strand, within GFF2795 at 319.489 kb on + strand, within GFF2795 at 319.600 kb on + strand, within GFF2795 at 319.615 kb on + strand, within GFF2795 at 319.700 kb on - strand, within GFF2795 at 319.799 kb on - strand, within GFF2795 at 319.868 kb on - strand, within GFF2795 at 319.939 kb on + strand, within GFF2795 at 320.065 kb on + strand at 320.065 kb on + strand at 320.115 kb on - strand at 320.145 kb on - strand at 320.178 kb on + strand, within GFF2796 at 320.178 kb on + strand, within GFF2796 at 320.178 kb on + strand, within GFF2796 at 320.178 kb on + strand, within GFF2796 at 320.222 kb on - strand, within GFF2796 at 320.264 kb on - strand at 320.298 kb on - strand at 320.504 kb on - strand at 320.564 kb on - strand, within GFF2797 at 320.713 kb on + strand, within GFF2797 at 320.795 kb on + strand, within GFF2797 at 320.938 kb on + strand, within GFF2797 at 320.957 kb on - strand, within GFF2797 at 320.957 kb on - strand, within GFF2797 at 320.960 kb on + strand, within GFF2797 at 321.591 kb on + strand, within GFF2797 at 321.592 kb on - strand, within GFF2797 at 321.822 kb on - strand, within GFF2797 at 321.984 kb on + strand at 322.060 kb on + strand at 322.270 kb on - strand, within GFF2798 at 322.399 kb on + strand at 322.400 kb on - strand at 322.405 kb on + strand at 322.511 kb on - strand at 322.547 kb on - strand at 322.586 kb on + strand at 322.755 kb on + strand, within GFF2799 at 322.883 kb on - strand, within GFF2799 at 322.915 kb on + strand, within GFF2799 at 322.952 kb on + strand, within GFF2799 at 323.024 kb on + strand, within GFF2799 at 323.025 kb on - strand, within GFF2799
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 319,352 + GFF2795 0.39 +0.5 319,352 + GFF2795 0.39 -0.1 319,403 - GFF2795 0.43 +0.4 319,403 - GFF2795 0.43 +0.9 319,489 + GFF2795 0.50 +1.0 319,600 + GFF2795 0.59 -0.2 319,615 + GFF2795 0.60 -0.0 319,700 - GFF2795 0.67 +0.2 319,799 - GFF2795 0.74 +0.2 319,868 - GFF2795 0.80 +0.3 319,939 + GFF2795 0.85 +0.5 320,065 + +0.5 320,065 + +0.6 320,115 - +0.6 320,145 - -2.0 320,178 + GFF2796 0.30 -0.2 320,178 + GFF2796 0.30 -0.0 320,178 + GFF2796 0.30 +0.3 320,178 + GFF2796 0.30 +0.8 320,222 - GFF2796 0.68 +0.1 320,264 - +0.3 320,298 - +0.4 320,504 - -0.0 320,564 - GFF2797 0.13 +0.9 320,713 + GFF2797 0.22 -0.8 320,795 + GFF2797 0.27 +0.5 320,938 + GFF2797 0.35 +1.0 320,957 - GFF2797 0.36 -0.6 320,957 - GFF2797 0.36 +0.2 320,960 + GFF2797 0.36 +0.2 321,591 + GFF2797 0.73 +0.3 321,592 - GFF2797 0.73 +1.8 321,822 - GFF2797 0.87 +0.0 321,984 + -0.7 322,060 + +1.0 322,270 - GFF2798 0.61 -0.3 322,399 + +0.3 322,400 - +0.3 322,405 + +0.8 322,511 - -0.9 322,547 - +0.5 322,586 + -0.7 322,755 + GFF2799 0.30 +0.8 322,883 - GFF2799 0.47 -0.4 322,915 + GFF2799 0.51 +1.1 322,952 + GFF2799 0.56 -0.1 323,024 + GFF2799 0.66 +0.1 323,025 - GFF2799 0.66 -0.2
Or see this region's nucleotide sequence