Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2752 and GFF2753 are separated by 202 nucleotides GFF2753 and GFF2754 are separated by 90 nucleotides
GFF2752 - UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9), at 273,446 to 274,354
GFF2752
GFF2753 - Hnr protein, at 274,557 to 275,570
GFF2753
GFF2754 - UPF0028 protein YchK, at 275,661 to 276,557
GFF2754
Position (kb) 274
275
276 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 273.703 kb on - strand, within GFF2752 at 274.042 kb on + strand, within GFF2752 at 274.479 kb on + strand at 274.485 kb on + strand at 274.503 kb on + strand at 274.713 kb on + strand, within GFF2753 at 274.769 kb on - strand, within GFF2753 at 274.849 kb on - strand, within GFF2753 at 275.051 kb on + strand, within GFF2753 at 275.080 kb on - strand, within GFF2753 at 275.192 kb on + strand, within GFF2753 at 275.192 kb on + strand, within GFF2753 at 275.192 kb on + strand, within GFF2753 at 275.193 kb on - strand, within GFF2753 at 275.348 kb on + strand, within GFF2753 at 275.348 kb on + strand, within GFF2753 at 275.353 kb on - strand, within GFF2753 at 275.403 kb on + strand, within GFF2753 at 275.404 kb on - strand, within GFF2753 at 275.517 kb on + strand at 275.593 kb on - strand at 275.610 kb on + strand at 275.611 kb on - strand at 275.611 kb on - strand at 275.657 kb on + strand at 275.657 kb on + strand at 275.662 kb on + strand at 275.688 kb on - strand at 275.936 kb on + strand, within GFF2754 at 275.936 kb on + strand, within GFF2754 at 275.986 kb on - strand, within GFF2754 at 276.105 kb on + strand, within GFF2754 at 276.152 kb on + strand, within GFF2754 at 276.180 kb on + strand, within GFF2754 at 276.194 kb on + strand, within GFF2754 at 276.202 kb on - strand, within GFF2754 at 276.282 kb on - strand, within GFF2754 at 276.339 kb on + strand, within GFF2754 at 276.339 kb on + strand, within GFF2754 at 276.381 kb on + strand, within GFF2754 at 276.468 kb on - strand at 276.569 kb on + strand at 276.569 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 273,703 - GFF2752 0.28 +4.1 274,042 + GFF2752 0.66 +2.0 274,479 + +0.2 274,485 + +0.3 274,503 + +1.4 274,713 + GFF2753 0.15 +2.1 274,769 - GFF2753 0.21 +0.5 274,849 - GFF2753 0.29 +1.4 275,051 + GFF2753 0.49 +2.6 275,080 - GFF2753 0.52 -0.3 275,192 + GFF2753 0.63 +2.4 275,192 + GFF2753 0.63 +0.4 275,192 + GFF2753 0.63 +2.3 275,193 - GFF2753 0.63 +2.0 275,348 + GFF2753 0.78 +2.3 275,348 + GFF2753 0.78 +2.1 275,353 - GFF2753 0.79 +1.5 275,403 + GFF2753 0.83 +1.9 275,404 - GFF2753 0.84 +1.1 275,517 + +2.3 275,593 - -1.2 275,610 + +0.0 275,611 - -1.5 275,611 - -4.4 275,657 + +2.2 275,657 + +1.4 275,662 + +2.4 275,688 - -0.3 275,936 + GFF2754 0.31 +2.2 275,936 + GFF2754 0.31 +2.2 275,986 - GFF2754 0.36 -0.5 276,105 + GFF2754 0.49 +2.0 276,152 + GFF2754 0.55 +1.4 276,180 + GFF2754 0.58 +1.9 276,194 + GFF2754 0.59 +0.3 276,202 - GFF2754 0.60 +2.0 276,282 - GFF2754 0.69 +0.7 276,339 + GFF2754 0.76 +1.7 276,339 + GFF2754 0.76 +1.8 276,381 + GFF2754 0.80 +0.4 276,468 - +1.4 276,569 + +2.6 276,569 + +1.6
Or see this region's nucleotide sequence