Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2705 and GFF2706 are separated by 6 nucleotides GFF2706 and GFF2707 are separated by 149 nucleotides GFF2707 and GFF2708 are separated by 108 nucleotides GFF2708 and GFF2709 are separated by 55 nucleotides
GFF2705 - Heat shock (predicted periplasmic) protein YciM, precursor, at 227,133 to 228,302
GFF2705
GFF2706 - Inner membrane protein yciS, at 228,309 to 228,617
GFF2706
GFF2707 - Phosphatidylglycerophosphatase B (EC 3.1.3.27), at 228,767 to 229,531
GFF2707
GFF2708 - GTP cyclohydrolase II (EC 3.5.4.25), at 229,640 to 230,317
GFF2708
GFF2709 - Aconitate hydratase (EC 4.2.1.3) @ 2-methylisocitrate dehydratase (EC 4.2.1.99), at 230,373 to 233,048
GFF2709
Position (kb) 228
229
230 Strain fitness (log2 ratio) -1
0
1 at 228.498 kb on - strand, within GFF2706 at 228.498 kb on - strand, within GFF2706 at 228.503 kb on - strand, within GFF2706 at 228.528 kb on - strand, within GFF2706 at 228.528 kb on - strand, within GFF2706 at 228.681 kb on - strand at 228.681 kb on - strand at 228.747 kb on - strand at 228.820 kb on + strand at 228.862 kb on + strand, within GFF2707 at 228.866 kb on + strand, within GFF2707 at 228.866 kb on + strand, within GFF2707 at 228.867 kb on - strand, within GFF2707 at 229.186 kb on + strand, within GFF2707 at 229.337 kb on + strand, within GFF2707 at 229.338 kb on - strand, within GFF2707 at 229.551 kb on - strand at 229.555 kb on - strand at 229.623 kb on + strand at 229.674 kb on + strand at 230.307 kb on + strand at 230.307 kb on + strand at 230.308 kb on - strand at 230.312 kb on - strand at 230.329 kb on + strand at 230.385 kb on - strand at 230.391 kb on - strand at 230.425 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 228,498 - GFF2706 0.61 -0.9 228,498 - GFF2706 0.61 +0.5 228,503 - GFF2706 0.63 -0.0 228,528 - GFF2706 0.71 +0.0 228,528 - GFF2706 0.71 +1.5 228,681 - +0.2 228,681 - +1.5 228,747 - +0.0 228,820 + -1.0 228,862 + GFF2707 0.12 +0.0 228,866 + GFF2707 0.13 -1.1 228,866 + GFF2707 0.13 -0.5 228,867 - GFF2707 0.13 -1.3 229,186 + GFF2707 0.55 -0.2 229,337 + GFF2707 0.75 +0.0 229,338 - GFF2707 0.75 +0.0 229,551 - -0.2 229,555 - -1.2 229,623 + +0.3 229,674 + -0.6 230,307 + -1.7 230,307 + -0.1 230,308 - +0.5 230,312 - +0.0 230,329 + -0.7 230,385 - +0.6 230,391 - -0.1 230,425 + -1.3
Or see this region's nucleotide sequence