Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2520 and GFF2521 overlap by 4 nucleotides GFF2521 and GFF2522 overlap by 1 nucleotides GFF2522 and GFF2523 are separated by 5 nucleotides GFF2523 and GFF2524 are separated by 19 nucleotides
GFF2520 - Hydrogenase maturation factor hoxO, at 35,599 to 36,009
GFF2520
GFF2521 - Putative hydrogenase protein, at 36,006 to 36,305
GFF2521
GFF2522 - Hydrogenase maturation protease (EC 3.4.24.-), at 36,305 to 36,913
GFF2522
GFF2523 - Ni,Fe-hydrogenase I cytochrome b subunit, at 36,919 to 37,593
GFF2523
GFF2524 - Uptake hydrogenase large subunit (EC 1.12.99.6), at 37,613 to 39,415
GFF2524
Position (kb) 36
37
38 Strain fitness (log2 ratio) -1
0
1 at 36.299 kb on + strand at 36.402 kb on + strand, within GFF2522 at 36.402 kb on + strand, within GFF2522 at 36.403 kb on - strand, within GFF2522 at 36.660 kb on + strand, within GFF2522 at 36.976 kb on - strand at 36.996 kb on + strand, within GFF2523 at 36.997 kb on - strand, within GFF2523 at 37.143 kb on - strand, within GFF2523 at 37.226 kb on + strand, within GFF2523 at 37.227 kb on - strand, within GFF2523 at 37.227 kb on - strand, within GFF2523 at 37.230 kb on - strand, within GFF2523 at 37.491 kb on + strand, within GFF2523 at 37.554 kb on - strand at 37.557 kb on + strand at 37.557 kb on + strand at 37.558 kb on - strand at 37.559 kb on + strand at 37.559 kb on + strand at 37.559 kb on + strand at 37.559 kb on + strand at 37.560 kb on - strand at 37.657 kb on + strand at 37.830 kb on + strand, within GFF2524 at 37.831 kb on - strand, within GFF2524 at 37.831 kb on - strand, within GFF2524 at 38.053 kb on + strand, within GFF2524 at 38.054 kb on - strand, within GFF2524 at 38.349 kb on + strand, within GFF2524 at 38.349 kb on + strand, within GFF2524 at 38.349 kb on + strand, within GFF2524 at 38.349 kb on + strand, within GFF2524 at 38.355 kb on + strand, within GFF2524 at 38.440 kb on + strand, within GFF2524
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 36,299 + -0.7 36,402 + GFF2522 0.16 +0.7 36,402 + GFF2522 0.16 +0.1 36,403 - GFF2522 0.16 +0.0 36,660 + GFF2522 0.58 +0.3 36,976 - -0.1 36,996 + GFF2523 0.11 -0.6 36,997 - GFF2523 0.12 -1.2 37,143 - GFF2523 0.33 -1.0 37,226 + GFF2523 0.45 +0.5 37,227 - GFF2523 0.46 -0.1 37,227 - GFF2523 0.46 -0.6 37,230 - GFF2523 0.46 -0.3 37,491 + GFF2523 0.85 -1.3 37,554 - +0.0 37,557 + -0.5 37,557 + -0.2 37,558 - -0.9 37,559 + +0.1 37,559 + +0.1 37,559 + +0.0 37,559 + -0.4 37,560 - +0.8 37,657 + +0.4 37,830 + GFF2524 0.12 +0.4 37,831 - GFF2524 0.12 +0.9 37,831 - GFF2524 0.12 -0.6 38,053 + GFF2524 0.24 +0.8 38,054 - GFF2524 0.24 +0.8 38,349 + GFF2524 0.41 -0.3 38,349 + GFF2524 0.41 -0.0 38,349 + GFF2524 0.41 +0.6 38,349 + GFF2524 0.41 +0.4 38,355 + GFF2524 0.41 -0.8 38,440 + GFF2524 0.46 +0.5
Or see this region's nucleotide sequence