Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2461

Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2459 and GFF2460 are separated by 113 nucleotidesGFF2460 and GFF2461 are separated by 4 nucleotidesGFF2461 and GFF2462 are separated by 50 nucleotidesGFF2462 and GFF2463 are separated by 90 nucleotides GFF2459 - Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3), at 76,739 to 80,626 GFF2459 GFF2460 - hypothetical protein, at 80,740 to 80,877 GFF2460 GFF2461 - Transglycosylase, Slt family, at 80,882 to 82,426 GFF2461 GFF2462 - tRNA-specific adenosine-34 deaminase (EC 3.5.4.-), at 82,477 to 82,962 GFF2462 GFF2463 - Hypothetical protein, similar to phosphoserine phosphatase, at 83,053 to 83,688 GFF2463 Position (kb) 80 81 82 83Strain fitness (log2 ratio) -1 0 1at 80.007 kb on + strand, within GFF2459at 80.008 kb on - strand, within GFF2459at 80.114 kb on + strand, within GFF2459at 80.224 kb on + strand, within GFF2459at 80.293 kb on - strandat 80.432 kb on - strandat 80.445 kb on + strandat 80.517 kb on + strandat 80.518 kb on - strandat 80.518 kb on - strandat 80.528 kb on + strandat 80.531 kb on - strandat 80.531 kb on - strandat 80.575 kb on + strandat 80.626 kb on + strandat 80.642 kb on + strandat 80.681 kb on - strandat 80.683 kb on - strandat 80.683 kb on - strandat 81.047 kb on + strand, within GFF2461at 81.111 kb on - strand, within GFF2461at 81.111 kb on - strand, within GFF2461at 81.254 kb on + strand, within GFF2461at 81.254 kb on + strand, within GFF2461at 81.254 kb on + strand, within GFF2461at 81.338 kb on - strand, within GFF2461at 81.338 kb on - strand, within GFF2461at 81.338 kb on - strand, within GFF2461at 81.599 kb on - strand, within GFF2461at 81.732 kb on + strand, within GFF2461at 81.762 kb on + strand, within GFF2461at 81.786 kb on + strand, within GFF2461at 81.967 kb on + strand, within GFF2461at 81.967 kb on + strand, within GFF2461at 82.009 kb on + strand, within GFF2461at 82.036 kb on + strand, within GFF2461at 82.099 kb on + strand, within GFF2461at 82.134 kb on + strand, within GFF2461at 82.161 kb on + strand, within GFF2461at 82.194 kb on - strand, within GFF2461at 82.194 kb on - strand, within GFF2461at 82.199 kb on + strand, within GFF2461at 82.371 kb on - strandat 82.478 kb on + strandat 83.144 kb on + strand, within GFF2463at 83.293 kb on - strand, within GFF2463at 83.365 kb on + strand, within GFF2463at 83.389 kb on + strand, within GFF2463

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
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80,007 + GFF2459 0.84 +0.3
80,008 - GFF2459 0.84 -0.0
80,114 + GFF2459 0.87 -1.5
80,224 + GFF2459 0.90 -0.1
80,293 - -0.3
80,432 - -0.2
80,445 + +0.1
80,517 + -0.4
80,518 - -0.4
80,518 - -0.3
80,528 + -0.7
80,531 - -0.4
80,531 - -0.7
80,575 + -0.4
80,626 + -0.4
80,642 + +0.7
80,681 - +0.3
80,683 - -0.5
80,683 - +0.2
81,047 + GFF2461 0.11 +0.5
81,111 - GFF2461 0.15 -1.3
81,111 - GFF2461 0.15 -1.7
81,254 + GFF2461 0.24 -0.8
81,254 + GFF2461 0.24 -0.7
81,254 + GFF2461 0.24 +0.0
81,338 - GFF2461 0.30 -0.6
81,338 - GFF2461 0.30 +0.5
81,338 - GFF2461 0.30 -0.6
81,599 - GFF2461 0.46 -0.4
81,732 + GFF2461 0.55 -0.8
81,762 + GFF2461 0.57 +0.3
81,786 + GFF2461 0.59 -0.2
81,967 + GFF2461 0.70 -0.6
81,967 + GFF2461 0.70 -1.5
82,009 + GFF2461 0.73 -0.8
82,036 + GFF2461 0.75 -1.6
82,099 + GFF2461 0.79 +0.4
82,134 + GFF2461 0.81 -0.8
82,161 + GFF2461 0.83 -0.5
82,194 - GFF2461 0.85 -1.6
82,194 - GFF2461 0.85 -0.4
82,199 + GFF2461 0.85 -0.3
82,371 - +0.7
82,478 + -0.0
83,144 + GFF2463 0.14 -1.2
83,293 - GFF2463 0.38 +0.1
83,365 + GFF2463 0.49 -0.2
83,389 + GFF2463 0.53 -0.2

Or see this region's nucleotide sequence