Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2459 and GFF2460 are separated by 113 nucleotides GFF2460 and GFF2461 are separated by 4 nucleotides GFF2461 and GFF2462 are separated by 50 nucleotides GFF2462 and GFF2463 are separated by 90 nucleotides
GFF2459 - Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3), at 76,739 to 80,626
GFF2459
GFF2460 - hypothetical protein, at 80,740 to 80,877
GFF2460
GFF2461 - Transglycosylase, Slt family, at 80,882 to 82,426
GFF2461
GFF2462 - tRNA-specific adenosine-34 deaminase (EC 3.5.4.-), at 82,477 to 82,962
GFF2462
GFF2463 - Hypothetical protein, similar to phosphoserine phosphatase, at 83,053 to 83,688
GFF2463
Position (kb)
80
81
82
83 Strain fitness (log2 ratio)
-1
0
1 at 80.007 kb on + strand, within GFF2459 at 80.008 kb on - strand, within GFF2459 at 80.114 kb on + strand, within GFF2459 at 80.224 kb on + strand, within GFF2459 at 80.293 kb on - strand at 80.432 kb on - strand at 80.445 kb on + strand at 80.517 kb on + strand at 80.518 kb on - strand at 80.518 kb on - strand at 80.528 kb on + strand at 80.531 kb on - strand at 80.531 kb on - strand at 80.575 kb on + strand at 80.626 kb on + strand at 80.642 kb on + strand at 80.681 kb on - strand at 80.683 kb on - strand at 80.683 kb on - strand at 81.047 kb on + strand, within GFF2461 at 81.111 kb on - strand, within GFF2461 at 81.111 kb on - strand, within GFF2461 at 81.254 kb on + strand, within GFF2461 at 81.254 kb on + strand, within GFF2461 at 81.254 kb on + strand, within GFF2461 at 81.338 kb on - strand, within GFF2461 at 81.338 kb on - strand, within GFF2461 at 81.338 kb on - strand, within GFF2461 at 81.599 kb on - strand, within GFF2461 at 81.732 kb on + strand, within GFF2461 at 81.762 kb on + strand, within GFF2461 at 81.786 kb on + strand, within GFF2461 at 81.967 kb on + strand, within GFF2461 at 81.967 kb on + strand, within GFF2461 at 82.009 kb on + strand, within GFF2461 at 82.036 kb on + strand, within GFF2461 at 82.099 kb on + strand, within GFF2461 at 82.134 kb on + strand, within GFF2461 at 82.161 kb on + strand, within GFF2461 at 82.194 kb on - strand, within GFF2461 at 82.194 kb on - strand, within GFF2461 at 82.199 kb on + strand, within GFF2461 at 82.371 kb on - strand at 82.478 kb on + strand at 83.144 kb on + strand, within GFF2463 at 83.293 kb on - strand, within GFF2463 at 83.365 kb on + strand, within GFF2463 at 83.389 kb on + strand, within GFF2463
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 80,007 + GFF2459 0.84 +0.3 80,008 - GFF2459 0.84 -0.0 80,114 + GFF2459 0.87 -1.5 80,224 + GFF2459 0.90 -0.1 80,293 - -0.3 80,432 - -0.2 80,445 + +0.1 80,517 + -0.4 80,518 - -0.4 80,518 - -0.3 80,528 + -0.7 80,531 - -0.4 80,531 - -0.7 80,575 + -0.4 80,626 + -0.4 80,642 + +0.7 80,681 - +0.3 80,683 - -0.5 80,683 - +0.2 81,047 + GFF2461 0.11 +0.5 81,111 - GFF2461 0.15 -1.3 81,111 - GFF2461 0.15 -1.7 81,254 + GFF2461 0.24 -0.8 81,254 + GFF2461 0.24 -0.7 81,254 + GFF2461 0.24 +0.0 81,338 - GFF2461 0.30 -0.6 81,338 - GFF2461 0.30 +0.5 81,338 - GFF2461 0.30 -0.6 81,599 - GFF2461 0.46 -0.4 81,732 + GFF2461 0.55 -0.8 81,762 + GFF2461 0.57 +0.3 81,786 + GFF2461 0.59 -0.2 81,967 + GFF2461 0.70 -0.6 81,967 + GFF2461 0.70 -1.5 82,009 + GFF2461 0.73 -0.8 82,036 + GFF2461 0.75 -1.6 82,099 + GFF2461 0.79 +0.4 82,134 + GFF2461 0.81 -0.8 82,161 + GFF2461 0.83 -0.5 82,194 - GFF2461 0.85 -1.6 82,194 - GFF2461 0.85 -0.4 82,199 + GFF2461 0.85 -0.3 82,371 - +0.7 82,478 + -0.0 83,144 + GFF2463 0.14 -1.2 83,293 - GFF2463 0.38 +0.1 83,365 + GFF2463 0.49 -0.2 83,389 + GFF2463 0.53 -0.2
Or see this region's nucleotide sequence