Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2340 and GFF2341 are separated by 160 nucleotides GFF2341 and GFF2342 are separated by 210 nucleotides
GFF2339 - PTS system fructose-like IIB component 2 precursor (EC 2.7.1.69), at 130,881 to 131,234
GFF2339
GFF2340 - Transcriptional regulator, AraC family, at 131,221 to 132,072
GFF2340
GFF2341 - UPF0141 membrane protein YijP possibly required for phosphoethanolamine modification of lipopolysaccharide, at 132,233 to 133,966
GFF2341
GFF2342 - Phosphoenolpyruvate carboxylase (EC 4.1.1.31), at 134,177 to 136,828
GFF2342
Position (kb)
132
133
134 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 131.295 kb on + strand at 131.296 kb on - strand at 131.322 kb on - strand, within GFF2340 at 131.326 kb on - strand, within GFF2340 at 131.326 kb on - strand, within GFF2340 at 131.326 kb on - strand, within GFF2340 at 131.412 kb on + strand, within GFF2340 at 131.485 kb on - strand, within GFF2340 at 131.487 kb on - strand, within GFF2340 at 131.623 kb on + strand, within GFF2340 at 131.624 kb on - strand, within GFF2340 at 131.627 kb on - strand, within GFF2340 at 131.685 kb on + strand, within GFF2340 at 131.685 kb on + strand, within GFF2340 at 131.792 kb on - strand, within GFF2340 at 132.068 kb on + strand at 132.068 kb on + strand at 132.068 kb on + strand at 132.069 kb on - strand at 132.069 kb on - strand at 132.227 kb on + strand at 132.227 kb on + strand at 132.227 kb on + strand at 132.228 kb on - strand at 132.234 kb on + strand at 132.259 kb on - strand at 132.259 kb on - strand at 132.268 kb on + strand at 132.269 kb on - strand at 132.269 kb on - strand at 132.269 kb on - strand at 132.274 kb on - strand at 132.274 kb on - strand at 132.297 kb on + strand at 132.372 kb on + strand at 132.372 kb on + strand at 132.372 kb on + strand at 132.386 kb on + strand at 132.424 kb on - strand, within GFF2341 at 132.424 kb on - strand, within GFF2341 at 132.505 kb on + strand, within GFF2341 at 132.508 kb on - strand, within GFF2341 at 132.508 kb on - strand, within GFF2341 at 132.508 kb on - strand, within GFF2341 at 132.508 kb on - strand, within GFF2341 at 132.508 kb on - strand, within GFF2341 at 132.508 kb on - strand, within GFF2341 at 132.508 kb on - strand, within GFF2341 at 132.508 kb on - strand, within GFF2341 at 132.511 kb on + strand, within GFF2341 at 132.511 kb on + strand, within GFF2341 at 132.512 kb on - strand, within GFF2341 at 132.512 kb on - strand, within GFF2341 at 132.568 kb on - strand, within GFF2341 at 132.568 kb on - strand, within GFF2341 at 132.627 kb on + strand, within GFF2341 at 132.628 kb on - strand, within GFF2341 at 132.654 kb on + strand, within GFF2341 at 132.681 kb on + strand, within GFF2341 at 132.691 kb on - strand, within GFF2341 at 132.713 kb on + strand, within GFF2341 at 132.714 kb on - strand, within GFF2341 at 132.714 kb on - strand, within GFF2341 at 132.780 kb on + strand, within GFF2341 at 132.780 kb on + strand, within GFF2341 at 132.781 kb on - strand, within GFF2341 at 132.912 kb on + strand, within GFF2341 at 133.001 kb on - strand, within GFF2341 at 133.022 kb on - strand, within GFF2341 at 133.107 kb on + strand, within GFF2341 at 133.107 kb on + strand, within GFF2341 at 133.130 kb on - strand, within GFF2341 at 133.204 kb on - strand, within GFF2341 at 133.214 kb on - strand, within GFF2341 at 133.360 kb on - strand, within GFF2341 at 133.369 kb on + strand, within GFF2341 at 133.372 kb on + strand, within GFF2341 at 133.438 kb on + strand, within GFF2341 at 133.438 kb on + strand, within GFF2341 at 133.439 kb on - strand, within GFF2341 at 133.439 kb on - strand, within GFF2341 at 133.439 kb on - strand, within GFF2341 at 133.439 kb on - strand, within GFF2341 at 133.447 kb on + strand, within GFF2341 at 133.485 kb on + strand, within GFF2341 at 133.529 kb on - strand, within GFF2341 at 133.531 kb on - strand, within GFF2341 at 133.576 kb on - strand, within GFF2341 at 133.576 kb on - strand, within GFF2341 at 133.611 kb on + strand, within GFF2341 at 133.623 kb on + strand, within GFF2341 at 133.697 kb on + strand, within GFF2341 at 133.701 kb on + strand, within GFF2341 at 133.702 kb on - strand, within GFF2341 at 133.704 kb on + strand, within GFF2341 at 133.704 kb on + strand, within GFF2341 at 133.705 kb on - strand, within GFF2341 at 133.705 kb on - strand, within GFF2341 at 133.812 kb on + strand at 133.818 kb on - strand at 133.860 kb on + strand at 133.865 kb on - strand at 133.899 kb on + strand at 133.942 kb on + strand at 133.952 kb on + strand at 133.953 kb on - strand at 133.966 kb on + strand at 134.127 kb on + strand at 134.127 kb on + strand at 134.127 kb on + strand at 134.127 kb on + strand at 134.128 kb on - strand at 134.148 kb on - strand at 134.148 kb on - strand at 134.148 kb on - strand at 134.326 kb on - strand at 134.410 kb on + strand at 134.411 kb on - strand at 134.860 kb on + strand, within GFF2342 at 134.861 kb on - strand, within GFF2342 at 134.864 kb on - strand, within GFF2342
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 131,295 + +0.7 131,296 - +0.4 131,322 - GFF2340 0.12 +0.0 131,326 - GFF2340 0.12 +0.4 131,326 - GFF2340 0.12 -0.3 131,326 - GFF2340 0.12 -0.7 131,412 + GFF2340 0.22 -0.6 131,485 - GFF2340 0.31 -0.2 131,487 - GFF2340 0.31 -0.8 131,623 + GFF2340 0.47 -0.9 131,624 - GFF2340 0.47 -0.2 131,627 - GFF2340 0.48 -0.2 131,685 + GFF2340 0.54 -0.2 131,685 + GFF2340 0.54 -0.6 131,792 - GFF2340 0.67 -0.3 132,068 + -0.5 132,068 + -0.5 132,068 + +0.1 132,069 - -3.4 132,069 - -3.5 132,227 + +0.4 132,227 + +0.2 132,227 + +0.2 132,228 - -0.0 132,234 + +0.6 132,259 - -1.0 132,259 - -1.2 132,268 + +1.0 132,269 - +1.3 132,269 - +1.5 132,269 - +0.9 132,274 - +2.6 132,274 - +2.4 132,297 + +2.7 132,372 + +2.7 132,372 + +2.4 132,372 + +2.3 132,386 + +2.1 132,424 - GFF2341 0.11 +2.6 132,424 - GFF2341 0.11 +2.1 132,505 + GFF2341 0.16 +2.6 132,508 - GFF2341 0.16 +1.7 132,508 - GFF2341 0.16 +2.2 132,508 - GFF2341 0.16 +2.4 132,508 - GFF2341 0.16 +1.9 132,508 - GFF2341 0.16 +2.5 132,508 - GFF2341 0.16 +2.8 132,508 - GFF2341 0.16 +2.2 132,508 - GFF2341 0.16 +1.4 132,511 + GFF2341 0.16 +2.1 132,511 + GFF2341 0.16 +2.4 132,512 - GFF2341 0.16 +2.5 132,512 - GFF2341 0.16 +3.0 132,568 - GFF2341 0.19 +2.3 132,568 - GFF2341 0.19 +2.7 132,627 + GFF2341 0.23 +2.2 132,628 - GFF2341 0.23 +1.3 132,654 + GFF2341 0.24 +2.6 132,681 + GFF2341 0.26 +2.3 132,691 - GFF2341 0.26 +2.3 132,713 + GFF2341 0.28 +2.7 132,714 - GFF2341 0.28 +2.7 132,714 - GFF2341 0.28 +2.1 132,780 + GFF2341 0.32 +2.0 132,780 + GFF2341 0.32 +2.9 132,781 - GFF2341 0.32 +3.2 132,912 + GFF2341 0.39 +2.4 133,001 - GFF2341 0.44 +3.1 133,022 - GFF2341 0.46 +2.7 133,107 + GFF2341 0.50 +2.7 133,107 + GFF2341 0.50 +2.5 133,130 - GFF2341 0.52 +2.7 133,204 - GFF2341 0.56 +2.2 133,214 - GFF2341 0.57 +2.3 133,360 - GFF2341 0.65 +2.5 133,369 + GFF2341 0.66 +2.3 133,372 + GFF2341 0.66 +1.8 133,438 + GFF2341 0.69 +2.0 133,438 + GFF2341 0.69 +2.0 133,439 - GFF2341 0.70 +1.7 133,439 - GFF2341 0.70 +2.4 133,439 - GFF2341 0.70 +2.5 133,439 - GFF2341 0.70 +2.6 133,447 + GFF2341 0.70 +2.6 133,485 + GFF2341 0.72 +2.8 133,529 - GFF2341 0.75 +2.6 133,531 - GFF2341 0.75 +3.2 133,576 - GFF2341 0.77 +1.8 133,576 - GFF2341 0.77 +2.0 133,611 + GFF2341 0.79 +2.5 133,623 + GFF2341 0.80 +2.7 133,697 + GFF2341 0.84 +2.4 133,701 + GFF2341 0.85 +2.6 133,702 - GFF2341 0.85 +2.3 133,704 + GFF2341 0.85 +2.5 133,704 + GFF2341 0.85 +2.5 133,705 - GFF2341 0.85 +2.5 133,705 - GFF2341 0.85 +3.2 133,812 + +2.5 133,818 - +2.4 133,860 + +2.4 133,865 - +2.0 133,899 + +3.2 133,942 + +2.6 133,952 + +2.5 133,953 - +2.6 133,966 + +2.5 134,127 + +1.3 134,127 + +1.3 134,127 + +0.7 134,127 + +1.0 134,128 - -0.1 134,148 - +0.5 134,148 - +0.8 134,148 - -0.1 134,326 - -1.0 134,410 + -0.2 134,411 - -0.1 134,860 + GFF2342 0.26 +0.1 134,861 - GFF2342 0.26 +1.1 134,864 - GFF2342 0.26 +0.2
Or see this region's nucleotide sequence