Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2293 and GFF2294 are separated by 129 nucleotides GFF2294 and GFF2295 are separated by 255 nucleotides
GFF2293 - Autoinducer 2 (AI-2) kinase LsrK (EC 2.7.1.-), at 79,530 to 81,080
GFF2293
GFF2294 - LsrR, transcriptional repressor of lsr operon, at 81,210 to 82,169
GFF2294
GFF2295 - Autoinducer 2 (AI-2) ABC transport system, fused AI2 transporter subunits and ATP-binding component, at 82,425 to 83,960
GFF2295
Position (kb) 81
82
83 Strain fitness (log2 ratio) -2
-1
0
1 at 80.355 kb on + strand, within GFF2293 at 80.591 kb on + strand, within GFF2293 at 80.591 kb on + strand, within GFF2293 at 80.592 kb on - strand, within GFF2293 at 80.592 kb on - strand, within GFF2293 at 80.592 kb on - strand, within GFF2293 at 80.615 kb on + strand, within GFF2293 at 80.615 kb on + strand, within GFF2293 at 80.615 kb on + strand, within GFF2293 at 80.616 kb on - strand, within GFF2293 at 80.616 kb on - strand, within GFF2293 at 80.639 kb on - strand, within GFF2293 at 80.643 kb on - strand, within GFF2293 at 80.916 kb on - strand, within GFF2293 at 81.030 kb on + strand at 81.085 kb on + strand at 81.107 kb on + strand at 81.274 kb on + strand at 81.379 kb on - strand, within GFF2294 at 81.387 kb on - strand, within GFF2294 at 81.507 kb on + strand, within GFF2294 at 81.507 kb on + strand, within GFF2294 at 81.659 kb on + strand, within GFF2294 at 81.659 kb on + strand, within GFF2294 at 81.660 kb on - strand, within GFF2294 at 81.662 kb on + strand, within GFF2294 at 81.662 kb on + strand, within GFF2294 at 81.666 kb on + strand, within GFF2294 at 81.666 kb on + strand, within GFF2294 at 81.667 kb on - strand, within GFF2294 at 81.672 kb on - strand, within GFF2294 at 81.705 kb on - strand, within GFF2294 at 81.707 kb on - strand, within GFF2294 at 81.707 kb on - strand, within GFF2294 at 81.707 kb on - strand, within GFF2294 at 81.848 kb on + strand, within GFF2294 at 82.096 kb on + strand at 82.097 kb on - strand at 82.109 kb on + strand at 82.157 kb on + strand at 82.442 kb on + strand at 82.477 kb on - strand at 82.520 kb on + strand at 82.583 kb on - strand, within GFF2295 at 82.658 kb on - strand, within GFF2295 at 82.727 kb on - strand, within GFF2295 at 82.743 kb on - strand, within GFF2295 at 82.795 kb on + strand, within GFF2295 at 82.909 kb on + strand, within GFF2295 at 82.909 kb on + strand, within GFF2295 at 83.129 kb on + strand, within GFF2295 at 83.130 kb on - strand, within GFF2295
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 80,355 + GFF2293 0.53 -1.3 80,591 + GFF2293 0.68 -1.5 80,591 + GFF2293 0.68 -0.8 80,592 - GFF2293 0.68 -0.0 80,592 - GFF2293 0.68 -0.5 80,592 - GFF2293 0.68 -0.3 80,615 + GFF2293 0.70 +0.1 80,615 + GFF2293 0.70 -1.3 80,615 + GFF2293 0.70 -0.1 80,616 - GFF2293 0.70 -0.1 80,616 - GFF2293 0.70 +0.2 80,639 - GFF2293 0.72 -1.4 80,643 - GFF2293 0.72 -0.2 80,916 - GFF2293 0.89 +1.0 81,030 + +0.3 81,085 + +0.4 81,107 + -0.6 81,274 + -1.3 81,379 - GFF2294 0.18 -0.1 81,387 - GFF2294 0.18 -1.0 81,507 + GFF2294 0.31 -0.2 81,507 + GFF2294 0.31 +0.2 81,659 + GFF2294 0.47 -1.6 81,659 + GFF2294 0.47 -0.0 81,660 - GFF2294 0.47 +0.1 81,662 + GFF2294 0.47 -0.4 81,662 + GFF2294 0.47 -0.2 81,666 + GFF2294 0.47 -0.4 81,666 + GFF2294 0.47 -2.3 81,667 - GFF2294 0.48 -0.2 81,672 - GFF2294 0.48 +0.9 81,705 - GFF2294 0.52 -0.4 81,707 - GFF2294 0.52 -0.1 81,707 - GFF2294 0.52 -0.7 81,707 - GFF2294 0.52 -0.3 81,848 + GFF2294 0.66 -0.4 82,096 + -0.8 82,097 - -0.1 82,109 + -0.6 82,157 + -0.7 82,442 + -0.1 82,477 - -0.3 82,520 + +0.1 82,583 - GFF2295 0.10 +0.8 82,658 - GFF2295 0.15 +0.7 82,727 - GFF2295 0.20 +0.3 82,743 - GFF2295 0.21 +0.2 82,795 + GFF2295 0.24 -0.3 82,909 + GFF2295 0.32 -0.0 82,909 + GFF2295 0.32 -0.3 83,129 + GFF2295 0.46 +1.0 83,130 - GFF2295 0.46 +0.4
Or see this region's nucleotide sequence