Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2256 and GFF2257 overlap by 4 nucleotides GFF2257 and GFF2258 are separated by 229 nucleotides GFF2258 and GFF2259 overlap by 1 nucleotides GFF2259 and GFF2260 are separated by 100 nucleotides
GFF2256 - FIG00638858: hypothetical protein, at 49,245 to 50,345
GFF2256
GFF2257 - hypothetical protein, at 50,342 to 50,470
GFF2257
GFF2258 - Inner membrane protein, at 50,700 to 51,023
GFF2258
GFF2259 - FIG00350115: hypothetical protein, at 51,023 to 51,682
GFF2259
GFF2260 - Transcriptional regulator, at 51,783 to 52,331
GFF2260
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-2
-1
0
1 at 50.130 kb on - strand, within GFF2256 at 50.172 kb on + strand, within GFF2256 at 50.172 kb on + strand, within GFF2256 at 50.446 kb on - strand, within GFF2257 at 50.568 kb on - strand at 50.568 kb on - strand at 50.667 kb on - strand at 50.668 kb on + strand at 50.668 kb on + strand at 50.701 kb on + strand at 50.709 kb on - strand at 50.775 kb on - strand, within GFF2258 at 50.875 kb on - strand, within GFF2258 at 50.875 kb on - strand, within GFF2258 at 50.969 kb on - strand, within GFF2258 at 51.003 kb on - strand at 51.307 kb on + strand, within GFF2259 at 51.362 kb on - strand, within GFF2259 at 51.362 kb on - strand, within GFF2259 at 51.364 kb on + strand, within GFF2259 at 51.509 kb on - strand, within GFF2259 at 51.573 kb on + strand, within GFF2259 at 51.645 kb on - strand at 51.715 kb on + strand at 51.782 kb on + strand at 51.794 kb on + strand at 51.794 kb on + strand at 51.794 kb on + strand at 51.794 kb on + strand at 51.794 kb on + strand at 51.888 kb on - strand, within GFF2260
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 50,130 - GFF2256 0.80 -0.2 50,172 + GFF2256 0.84 +0.2 50,172 + GFF2256 0.84 -0.2 50,446 - GFF2257 0.81 +0.3 50,568 - +0.4 50,568 - -0.7 50,667 - -0.2 50,668 + +0.7 50,668 + +0.6 50,701 + +0.8 50,709 - -0.5 50,775 - GFF2258 0.23 -1.5 50,875 - GFF2258 0.54 +0.1 50,875 - GFF2258 0.54 +0.8 50,969 - GFF2258 0.83 -2.1 51,003 - +0.1 51,307 + GFF2259 0.43 -0.9 51,362 - GFF2259 0.51 +0.3 51,362 - GFF2259 0.51 +0.9 51,364 + GFF2259 0.52 -0.6 51,509 - GFF2259 0.74 +0.3 51,573 + GFF2259 0.83 -1.1 51,645 - -0.3 51,715 + -0.3 51,782 + +0.3 51,794 + +0.1 51,794 + -0.5 51,794 + -0.3 51,794 + -0.0 51,794 + +1.7 51,888 - GFF2260 0.19 +1.0
Or see this region's nucleotide sequence