Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2168

Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2167 and GFF2168 are separated by 188 nucleotidesGFF2168 and GFF2169 overlap by 1 nucleotides GFF2167 - Polyphosphate kinase (EC 2.7.4.1), at 14,381 to 16,447 GFF2167 GFF2168 - Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2), at 16,636 to 17,274 GFF2168 GFF2169 - Phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1), at 17,274 to 18,311 GFF2169 Position (kb) 16 17 18Strain fitness (log2 ratio) -2 -1 0 1 2at 15.657 kb on + strand, within GFF2167at 15.988 kb on - strand, within GFF2167at 16.045 kb on + strand, within GFF2167at 16.045 kb on + strand, within GFF2167at 16.067 kb on - strand, within GFF2167at 16.428 kb on + strandat 16.429 kb on - strandat 16.452 kb on - strandat 16.456 kb on - strandat 16.467 kb on + strandat 16.489 kb on - strandat 16.505 kb on - strandat 16.511 kb on + strandat 16.634 kb on + strandat 16.708 kb on + strand, within GFF2168at 16.731 kb on + strand, within GFF2168at 16.732 kb on - strand, within GFF2168at 16.782 kb on + strand, within GFF2168at 16.783 kb on - strand, within GFF2168at 17.004 kb on + strand, within GFF2168at 17.004 kb on + strand, within GFF2168at 17.042 kb on + strand, within GFF2168at 17.234 kb on - strandat 17.234 kb on - strandat 17.269 kb on - strandat 17.424 kb on - strand, within GFF2169at 17.429 kb on - strand, within GFF2169at 17.429 kb on - strand, within GFF2169at 17.451 kb on - strand, within GFF2169at 17.505 kb on - strand, within GFF2169at 17.587 kb on - strand, within GFF2169at 17.587 kb on - strand, within GFF2169at 17.627 kb on - strand, within GFF2169at 17.630 kb on + strand, within GFF2169at 17.630 kb on + strand, within GFF2169at 17.631 kb on - strand, within GFF2169at 17.711 kb on + strand, within GFF2169at 17.866 kb on + strand, within GFF2169at 17.867 kb on - strand, within GFF2169at 17.867 kb on - strand, within GFF2169at 17.989 kb on + strand, within GFF2169at 18.111 kb on + strand, within GFF2169at 18.226 kb on - strandat 18.226 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
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15,657 + GFF2167 0.62 -1.1
15,988 - GFF2167 0.78 +0.3
16,045 + GFF2167 0.81 -2.0
16,045 + GFF2167 0.81 -0.7
16,067 - GFF2167 0.82 +0.7
16,428 + +0.7
16,429 - +1.0
16,452 - +1.1
16,456 - +0.6
16,467 + +0.5
16,489 - -1.0
16,505 - -0.0
16,511 + +1.1
16,634 + -0.1
16,708 + GFF2168 0.11 +0.8
16,731 + GFF2168 0.15 +1.0
16,732 - GFF2168 0.15 +1.8
16,782 + GFF2168 0.23 +0.3
16,783 - GFF2168 0.23 -0.4
17,004 + GFF2168 0.58 +0.3
17,004 + GFF2168 0.58 -1.3
17,042 + GFF2168 0.64 +0.1
17,234 - +2.7
17,234 - +0.6
17,269 - +0.3
17,424 - GFF2169 0.14 -0.5
17,429 - GFF2169 0.15 +0.0
17,429 - GFF2169 0.15 -1.1
17,451 - GFF2169 0.17 +0.8
17,505 - GFF2169 0.22 -0.6
17,587 - GFF2169 0.30 -0.1
17,587 - GFF2169 0.30 -1.2
17,627 - GFF2169 0.34 +0.5
17,630 + GFF2169 0.34 -0.2
17,630 + GFF2169 0.34 -0.2
17,631 - GFF2169 0.34 -0.0
17,711 + GFF2169 0.42 -0.1
17,866 + GFF2169 0.57 -0.2
17,867 - GFF2169 0.57 -0.4
17,867 - GFF2169 0.57 +0.5
17,989 + GFF2169 0.69 +1.4
18,111 + GFF2169 0.81 +0.5
18,226 - -1.0
18,226 - +0.3

Or see this region's nucleotide sequence