Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF211 and GFF212 are separated by 76 nucleotides GFF212 and GFF213 are separated by 273 nucleotides GFF213 and GFF214 are separated by 18 nucleotides
GFF211 - Carnitine operon protein CaiE, at 207,474 to 208,070
GFF211
GFF212 - Transcriptional activatory protein CaiF, at 208,147 to 208,542
GFF212
GFF213 - Carbamoyl-phosphate synthase large chain (EC 6.3.5.5), at 208,816 to 212,043
GFF213
GFF214 - Carbamoyl-phosphate synthase small chain (EC 6.3.5.5), at 212,062 to 213,210
GFF214
Position (kb) 208
209
210
211
212
213 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 207.896 kb on - strand, within GFF211 at 207.948 kb on + strand, within GFF211 at 208.069 kb on - strand at 208.069 kb on - strand at 208.114 kb on + strand at 208.403 kb on + strand, within GFF212 at 208.680 kb on - strand at 208.772 kb on + strand at 208.774 kb on + strand at 208.775 kb on - strand at 208.859 kb on - strand at 208.926 kb on - strand at 208.935 kb on + strand at 209.005 kb on - strand at 209.005 kb on - strand at 209.005 kb on - strand at 209.005 kb on - strand at 209.009 kb on + strand at 209.009 kb on + strand at 209.009 kb on + strand at 209.010 kb on - strand at 209.010 kb on - strand at 209.010 kb on - strand at 209.010 kb on - strand at 209.010 kb on - strand at 209.013 kb on + strand at 209.013 kb on + strand at 209.013 kb on + strand at 209.014 kb on - strand at 209.014 kb on - strand at 209.014 kb on - strand at 209.014 kb on - strand at 209.014 kb on - strand at 209.089 kb on - strand at 209.323 kb on + strand, within GFF213 at 209.649 kb on - strand, within GFF213 at 209.709 kb on + strand, within GFF213 at 209.710 kb on - strand, within GFF213 at 209.902 kb on + strand, within GFF213 at 210.241 kb on - strand, within GFF213 at 210.252 kb on + strand, within GFF213 at 210.295 kb on + strand, within GFF213 at 210.295 kb on + strand, within GFF213 at 210.413 kb on + strand, within GFF213 at 210.417 kb on + strand, within GFF213 at 210.417 kb on + strand, within GFF213 at 210.418 kb on - strand, within GFF213 at 210.418 kb on - strand, within GFF213 at 210.418 kb on - strand, within GFF213 at 210.418 kb on - strand, within GFF213 at 210.419 kb on + strand, within GFF213 at 210.420 kb on - strand, within GFF213 at 210.486 kb on - strand, within GFF213 at 210.732 kb on - strand, within GFF213 at 211.591 kb on - strand, within GFF213 at 211.671 kb on + strand, within GFF213 at 211.850 kb on + strand at 211.941 kb on + strand at 211.942 kb on - strand at 211.962 kb on - strand at 212.024 kb on - strand at 212.031 kb on + strand at 212.031 kb on + strand at 212.063 kb on + strand at 212.081 kb on + strand at 212.091 kb on + strand at 212.092 kb on - strand at 212.314 kb on - strand, within GFF214 at 212.359 kb on - strand, within GFF214 at 212.390 kb on - strand, within GFF214 at 212.590 kb on - strand, within GFF214 at 212.751 kb on + strand, within GFF214
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 207,896 - GFF211 0.71 +0.6 207,948 + GFF211 0.79 -0.0 208,069 - -0.0 208,069 - +0.0 208,114 + -0.5 208,403 + GFF212 0.65 +3.3 208,680 - +0.2 208,772 + -0.0 208,774 + +0.1 208,775 - -0.2 208,859 - -2.3 208,926 - -0.7 208,935 + -0.6 209,005 - +0.8 209,005 - -0.2 209,005 - -0.9 209,005 - -1.1 209,009 + -2.0 209,009 + -1.0 209,009 + -1.1 209,010 - -0.0 209,010 - -0.3 209,010 - -0.7 209,010 - -0.0 209,010 - -0.7 209,013 + -1.0 209,013 + -0.3 209,013 + -1.0 209,014 - +1.0 209,014 - -0.7 209,014 - -0.7 209,014 - -0.9 209,014 - -0.4 209,089 - +0.1 209,323 + GFF213 0.16 -0.3 209,649 - GFF213 0.26 -0.0 209,709 + GFF213 0.28 -0.0 209,710 - GFF213 0.28 -1.3 209,902 + GFF213 0.34 -0.9 210,241 - GFF213 0.44 -0.2 210,252 + GFF213 0.44 +0.7 210,295 + GFF213 0.46 -1.9 210,295 + GFF213 0.46 -1.1 210,413 + GFF213 0.49 +1.3 210,417 + GFF213 0.50 +0.3 210,417 + GFF213 0.50 -1.6 210,418 - GFF213 0.50 -0.7 210,418 - GFF213 0.50 -1.0 210,418 - GFF213 0.50 -1.6 210,418 - GFF213 0.50 -0.4 210,419 + GFF213 0.50 +0.3 210,420 - GFF213 0.50 -0.9 210,486 - GFF213 0.52 -0.7 210,732 - GFF213 0.59 -1.2 211,591 - GFF213 0.86 -0.0 211,671 + GFF213 0.88 -1.0 211,850 + -0.9 211,941 + -1.0 211,942 - -1.2 211,962 - +0.3 212,024 - -2.1 212,031 + -0.9 212,031 + -2.6 212,063 + -1.6 212,081 + -0.7 212,091 + +0.4 212,092 - -3.1 212,314 - GFF214 0.22 -1.8 212,359 - GFF214 0.26 +0.5 212,390 - GFF214 0.29 -2.2 212,590 - GFF214 0.46 -1.4 212,751 + GFF214 0.60 -0.9
Or see this region's nucleotide sequence