Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2120 and GFF2121 are separated by 150 nucleotides GFF2121 and GFF2122 are separated by 82 nucleotides GFF2122 and GFF2123 are separated by 85 nucleotides GFF2123 and GFF2124 are separated by 25 nucleotides
GFF2120 - FIG00554530: hypothetical protein, at 6,894 to 8,993
GFF2120
GFF2121 - FIG00638667: hypothetical protein, at 9,144 to 9,308
GFF2121
GFF2122 - Adenine-specific methyltransferase (EC 2.1.1.72), at 9,391 to 10,275
GFF2122
GFF2123 - DNA-binding protein Fis, at 10,361 to 10,657
GFF2123
GFF2124 - tRNA dihydrouridine synthase B (EC 1.-.-.-), at 10,683 to 11,528
GFF2124
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 8.442 kb on - strand, within GFF2120 at 8.445 kb on + strand, within GFF2120 at 8.552 kb on + strand, within GFF2120 at 8.626 kb on + strand, within GFF2120 at 8.626 kb on + strand, within GFF2120 at 8.626 kb on + strand, within GFF2120 at 8.868 kb on + strand at 8.869 kb on - strand at 8.916 kb on + strand at 8.916 kb on + strand at 8.927 kb on - strand at 9.057 kb on - strand at 9.117 kb on + strand at 9.299 kb on + strand at 9.303 kb on + strand at 9.303 kb on + strand at 9.303 kb on + strand at 9.304 kb on - strand at 9.348 kb on + strand at 9.349 kb on - strand at 9.350 kb on + strand at 9.653 kb on - strand, within GFF2122 at 9.702 kb on - strand, within GFF2122 at 9.716 kb on + strand, within GFF2122 at 9.717 kb on - strand, within GFF2122 at 9.767 kb on + strand, within GFF2122 at 9.897 kb on - strand, within GFF2122 at 9.900 kb on + strand, within GFF2122 at 9.900 kb on + strand, within GFF2122 at 9.936 kb on + strand, within GFF2122 at 9.937 kb on - strand, within GFF2122 at 9.937 kb on - strand, within GFF2122 at 9.941 kb on + strand, within GFF2122 at 9.941 kb on + strand, within GFF2122 at 9.999 kb on - strand, within GFF2122 at 10.028 kb on + strand, within GFF2122 at 10.029 kb on - strand, within GFF2122 at 10.080 kb on - strand, within GFF2122 at 10.507 kb on - strand, within GFF2123 at 10.629 kb on - strand at 10.809 kb on - strand, within GFF2124 at 11.004 kb on - strand, within GFF2124 at 11.037 kb on - strand, within GFF2124 at 11.081 kb on + strand, within GFF2124 at 11.194 kb on - strand, within GFF2124 at 11.202 kb on - strand, within GFF2124
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 8,442 - GFF2120 0.74 -1.3 8,445 + GFF2120 0.74 +0.1 8,552 + GFF2120 0.79 -0.3 8,626 + GFF2120 0.82 +0.2 8,626 + GFF2120 0.82 +0.1 8,626 + GFF2120 0.82 +0.3 8,868 + -0.8 8,869 - -0.0 8,916 + -0.8 8,916 + -1.0 8,927 - -0.2 9,057 - -0.3 9,117 + +0.4 9,299 + -0.2 9,303 + +0.5 9,303 + -0.2 9,303 + -1.3 9,304 - +0.0 9,348 + -1.5 9,349 - -0.3 9,350 + +0.6 9,653 - GFF2122 0.30 -0.5 9,702 - GFF2122 0.35 -0.2 9,716 + GFF2122 0.37 -0.4 9,717 - GFF2122 0.37 +0.2 9,767 + GFF2122 0.42 +0.3 9,897 - GFF2122 0.57 -0.6 9,900 + GFF2122 0.58 +0.6 9,900 + GFF2122 0.58 -0.0 9,936 + GFF2122 0.62 -0.1 9,937 - GFF2122 0.62 -0.0 9,937 - GFF2122 0.62 -0.1 9,941 + GFF2122 0.62 +0.2 9,941 + GFF2122 0.62 -0.3 9,999 - GFF2122 0.69 -0.1 10,028 + GFF2122 0.72 -0.2 10,029 - GFF2122 0.72 -0.2 10,080 - GFF2122 0.78 -0.2 10,507 - GFF2123 0.49 -2.0 10,629 - -1.0 10,809 - GFF2124 0.15 +2.1 11,004 - GFF2124 0.38 +2.9 11,037 - GFF2124 0.42 +0.6 11,081 + GFF2124 0.47 -2.8 11,194 - GFF2124 0.60 -1.1 11,202 - GFF2124 0.61 -0.5
Or see this region's nucleotide sequence