Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2049 and GFF2050 overlap by 4 nucleotides GFF2050 and GFF2051 are separated by 82 nucleotides GFF2051 and GFF2052 are separated by 14 nucleotides GFF2052 and GFF2053 are separated by 58 nucleotides
GFF2049 - FIG01045978: hypothetical protein, at 15,337 to 15,576
GFF2049
GFF2050 - Pyridoxal kinase (EC 2.7.1.35), at 15,573 to 16,439
GFF2050
GFF2051 - Predicted transcriptional regulator of pyridoxine metabolism, at 16,522 to 17,814
GFF2051
GFF2052 - Putative amidotransferase, at 17,829 to 18,548
GFF2052
GFF2053 - hypothetical protein, at 18,607 to 18,987
GFF2053
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-2
-1
0
1 at 15.945 kb on + strand, within GFF2050 at 15.945 kb on + strand, within GFF2050 at 15.945 kb on + strand, within GFF2050 at 16.019 kb on - strand, within GFF2050 at 16.062 kb on - strand, within GFF2050 at 16.062 kb on - strand, within GFF2050 at 16.062 kb on - strand, within GFF2050 at 16.139 kb on - strand, within GFF2050 at 16.307 kb on + strand, within GFF2050 at 16.480 kb on - strand at 16.670 kb on - strand, within GFF2051 at 16.672 kb on - strand, within GFF2051 at 16.971 kb on + strand, within GFF2051 at 17.055 kb on + strand, within GFF2051 at 17.261 kb on + strand, within GFF2051 at 17.351 kb on + strand, within GFF2051 at 17.351 kb on + strand, within GFF2051 at 17.394 kb on + strand, within GFF2051 at 17.394 kb on + strand, within GFF2051 at 17.513 kb on - strand, within GFF2051 at 17.513 kb on - strand, within GFF2051 at 17.646 kb on + strand, within GFF2051 at 17.739 kb on + strand at 17.928 kb on + strand, within GFF2052 at 18.182 kb on + strand, within GFF2052 at 18.183 kb on - strand, within GFF2052 at 18.183 kb on - strand, within GFF2052 at 18.264 kb on - strand, within GFF2052 at 18.325 kb on + strand, within GFF2052 at 18.366 kb on - strand, within GFF2052 at 18.447 kb on - strand, within GFF2052
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 15,945 + GFF2050 0.43 -0.4 15,945 + GFF2050 0.43 -0.5 15,945 + GFF2050 0.43 +0.3 16,019 - GFF2050 0.51 -0.4 16,062 - GFF2050 0.56 -1.4 16,062 - GFF2050 0.56 -0.9 16,062 - GFF2050 0.56 -0.5 16,139 - GFF2050 0.65 -0.4 16,307 + GFF2050 0.85 +0.8 16,480 - -0.1 16,670 - GFF2051 0.11 -1.0 16,672 - GFF2051 0.12 -1.4 16,971 + GFF2051 0.35 -1.0 17,055 + GFF2051 0.41 -0.7 17,261 + GFF2051 0.57 -0.9 17,351 + GFF2051 0.64 -1.9 17,351 + GFF2051 0.64 +0.2 17,394 + GFF2051 0.67 -1.0 17,394 + GFF2051 0.67 -1.9 17,513 - GFF2051 0.77 -1.4 17,513 - GFF2051 0.77 -0.5 17,646 + GFF2051 0.87 -0.9 17,739 + +0.8 17,928 + GFF2052 0.14 +0.6 18,182 + GFF2052 0.49 +0.5 18,183 - GFF2052 0.49 -0.4 18,183 - GFF2052 0.49 +0.1 18,264 - GFF2052 0.60 +0.4 18,325 + GFF2052 0.69 +0.1 18,366 - GFF2052 0.75 -0.2 18,447 - GFF2052 0.86 -0.9
Or see this region's nucleotide sequence