Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2000 and GFF2001 are separated by 16 nucleotides GFF2001 and GFF2002 are separated by 47 nucleotides GFF2002 and GFF2003 are separated by 80 nucleotides GFF2003 and GFF2004 are separated by 284 nucleotides GFF2004 and GFF2005 are separated by 21 nucleotides
GFF2000 - Outer membrane lipoprotein Blc, at 14,107 to 14,640
GFF2000
GFF2001 - Quaternary ammonium compound-resistance protein SugE, at 14,657 to 14,974
GFF2001
GFF2002 - hypothetical protein, at 15,022 to 15,150
GFF2002
GFF2003 - Putative luxR family bacterial regulatory protein, at 15,231 to 15,818
GFF2003
GFF2004 - Entericidin B precursor, at 16,103 to 16,276
GFF2004
GFF2005 - Translation elongation factor P, at 16,298 to 16,864
GFF2005
Position (kb) 15
16 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 14.237 kb on - strand, within GFF2000 at 14.296 kb on + strand, within GFF2000 at 14.296 kb on + strand, within GFF2000 at 14.455 kb on - strand, within GFF2000 at 14.613 kb on - strand at 14.638 kb on + strand at 14.800 kb on - strand, within GFF2001 at 14.957 kb on + strand at 14.974 kb on + strand at 14.988 kb on + strand at 14.992 kb on + strand at 14.993 kb on - strand at 15.017 kb on - strand at 15.023 kb on + strand at 15.024 kb on - strand at 15.042 kb on + strand, within GFF2002 at 15.043 kb on - strand, within GFF2002 at 15.043 kb on - strand, within GFF2002 at 15.139 kb on + strand at 15.140 kb on - strand at 15.152 kb on + strand at 15.173 kb on - strand at 15.328 kb on + strand, within GFF2003 at 15.348 kb on - strand, within GFF2003 at 15.348 kb on - strand, within GFF2003 at 15.348 kb on - strand, within GFF2003 at 15.349 kb on + strand, within GFF2003 at 15.365 kb on + strand, within GFF2003 at 15.440 kb on + strand, within GFF2003 at 15.487 kb on + strand, within GFF2003 at 15.488 kb on - strand, within GFF2003 at 15.508 kb on - strand, within GFF2003 at 15.556 kb on + strand, within GFF2003 at 15.589 kb on + strand, within GFF2003 at 15.594 kb on - strand, within GFF2003 at 15.702 kb on + strand, within GFF2003 at 15.739 kb on - strand, within GFF2003 at 15.739 kb on - strand, within GFF2003 at 15.741 kb on - strand, within GFF2003 at 15.746 kb on + strand, within GFF2003 at 15.767 kb on + strand at 15.768 kb on - strand at 15.768 kb on - strand at 15.794 kb on + strand at 15.887 kb on + strand at 15.929 kb on + strand at 15.940 kb on - strand at 15.942 kb on + strand at 15.996 kb on - strand at 15.996 kb on - strand at 16.030 kb on - strand at 16.031 kb on + strand at 16.039 kb on + strand at 16.172 kb on - strand, within GFF2004 at 16.184 kb on + strand, within GFF2004 at 16.185 kb on - strand, within GFF2004 at 16.195 kb on + strand, within GFF2004 at 16.196 kb on - strand, within GFF2004 at 16.295 kb on - strand at 16.295 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 14,237 - GFF2000 0.24 +1.1 14,296 + GFF2000 0.35 +0.1 14,296 + GFF2000 0.35 -0.6 14,455 - GFF2000 0.65 +0.0 14,613 - -0.5 14,638 + -0.1 14,800 - GFF2001 0.45 +0.3 14,957 + +0.4 14,974 + -0.7 14,988 + -0.8 14,992 + -0.7 14,993 - +0.7 15,017 - +0.1 15,023 + +0.4 15,024 - +0.3 15,042 + GFF2002 0.16 +0.1 15,043 - GFF2002 0.16 +0.7 15,043 - GFF2002 0.16 +0.5 15,139 + +0.8 15,140 - -0.2 15,152 + +1.7 15,173 - +0.5 15,328 + GFF2003 0.16 -1.1 15,348 - GFF2003 0.20 -1.1 15,348 - GFF2003 0.20 -3.2 15,348 - GFF2003 0.20 +0.1 15,349 + GFF2003 0.20 -0.5 15,365 + GFF2003 0.23 -0.6 15,440 + GFF2003 0.36 -0.9 15,487 + GFF2003 0.44 -0.2 15,488 - GFF2003 0.44 -0.8 15,508 - GFF2003 0.47 -0.2 15,556 + GFF2003 0.55 -1.2 15,589 + GFF2003 0.61 -0.5 15,594 - GFF2003 0.62 -0.4 15,702 + GFF2003 0.80 -0.5 15,739 - GFF2003 0.86 +0.2 15,739 - GFF2003 0.86 -0.5 15,741 - GFF2003 0.87 +0.0 15,746 + GFF2003 0.88 -0.4 15,767 + -0.0 15,768 - -0.9 15,768 - -0.3 15,794 + -0.4 15,887 + +0.6 15,929 + -0.2 15,940 - +0.1 15,942 + +0.4 15,996 - -0.6 15,996 - +0.1 16,030 - +0.1 16,031 + +0.0 16,039 + -0.0 16,172 - GFF2004 0.40 -0.4 16,184 + GFF2004 0.47 +0.1 16,185 - GFF2004 0.47 +0.1 16,195 + GFF2004 0.53 -0.4 16,196 - GFF2004 0.53 +0.8 16,295 - +0.8 16,295 - +0.7
Or see this region's nucleotide sequence