Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1648

Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1647 and GFF1648 are separated by 324 nucleotidesGFF1648 and GFF1649 overlap by 4 nucleotidesGFF1649 and GFF1650 are separated by 52 nucleotides GFF1647 - Aspartyl-tRNA synthetase (EC 6.1.1.12), at 31,532 to 33,304 GFF1647 GFF1648 - Nicotinamidase/isochorismatase family protein, at 33,629 to 34,195 GFF1648 GFF1649 - FIG003003: hypothetical protein, at 34,192 to 35,010 GFF1649 GFF1650 - FIG01046209: inner membrane protein, at 35,063 to 35,458 GFF1650 Position (kb) 33 34 35Strain fitness (log2 ratio) -1 0 1at 33.460 kb on - strandat 33.498 kb on - strandat 33.784 kb on + strand, within GFF1648at 33.785 kb on - strand, within GFF1648at 33.812 kb on + strand, within GFF1648at 33.928 kb on + strand, within GFF1648at 34.138 kb on + strand, within GFF1648at 34.199 kb on - strandat 34.566 kb on + strand, within GFF1649at 34.567 kb on - strand, within GFF1649at 34.578 kb on - strand, within GFF1649at 34.641 kb on - strand, within GFF1649at 34.685 kb on + strand, within GFF1649at 34.686 kb on - strand, within GFF1649at 35.026 kb on + strandat 35.027 kb on - strandat 35.078 kb on + strandat 35.144 kb on + strand, within GFF1650

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
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33,460 - -0.2
33,498 - +0.1
33,784 + GFF1648 0.27 +0.3
33,785 - GFF1648 0.28 -0.3
33,812 + GFF1648 0.32 -0.8
33,928 + GFF1648 0.53 -1.0
34,138 + GFF1648 0.90 -1.0
34,199 - +0.2
34,566 + GFF1649 0.46 +0.1
34,567 - GFF1649 0.46 -1.1
34,578 - GFF1649 0.47 +0.2
34,641 - GFF1649 0.55 +0.0
34,685 + GFF1649 0.60 -0.2
34,686 - GFF1649 0.60 -0.3
35,026 + -0.1
35,027 - -0.2
35,078 + -0.3
35,144 + GFF1650 0.20 -0.4

Or see this region's nucleotide sequence