Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1621 and GFF1622 are separated by 107 nucleotides GFF1622 and GFF1623 are separated by 23 nucleotides GFF1623 and GFF1624 are separated by 34 nucleotides
GFF1621 - DNA polymerase III theta subunit (EC 2.7.7.7), at 6,475 to 6,705
GFF1621
GFF1622 - Putative amidohydrolase, at 6,813 to 7,469
GFF1622
GFF1623 - Exodeoxyribonuclease X (EC 3.1.11.-), at 7,493 to 8,191
GFF1623
GFF1624 - Protease II (EC 3.4.21.83), at 8,226 to 10,277
GFF1624
Position (kb) 7
8
9 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 6.565 kb on + strand, within GFF1621 at 6.570 kb on + strand, within GFF1621 at 6.730 kb on + strand at 6.749 kb on + strand at 6.750 kb on - strand at 6.812 kb on + strand at 7.043 kb on + strand, within GFF1622 at 7.043 kb on + strand, within GFF1622 at 7.049 kb on + strand, within GFF1622 at 7.062 kb on - strand, within GFF1622 at 7.076 kb on + strand, within GFF1622 at 7.090 kb on + strand, within GFF1622 at 7.104 kb on - strand, within GFF1622 at 7.217 kb on + strand, within GFF1622 at 7.219 kb on + strand, within GFF1622 at 7.219 kb on + strand, within GFF1622 at 7.220 kb on - strand, within GFF1622 at 7.220 kb on - strand, within GFF1622 at 7.220 kb on - strand, within GFF1622 at 7.295 kb on - strand, within GFF1622 at 7.314 kb on + strand, within GFF1622 at 7.382 kb on + strand, within GFF1622 at 7.383 kb on - strand, within GFF1622 at 7.411 kb on + strand at 7.468 kb on - strand at 7.542 kb on + strand at 7.648 kb on + strand, within GFF1623 at 7.712 kb on - strand, within GFF1623 at 7.721 kb on + strand, within GFF1623 at 7.755 kb on + strand, within GFF1623 at 7.759 kb on - strand, within GFF1623 at 7.834 kb on + strand, within GFF1623 at 7.866 kb on + strand, within GFF1623 at 7.913 kb on + strand, within GFF1623 at 7.915 kb on + strand, within GFF1623 at 7.940 kb on - strand, within GFF1623 at 7.943 kb on - strand, within GFF1623 at 8.102 kb on - strand, within GFF1623 at 8.234 kb on + strand at 8.342 kb on + strand at 8.342 kb on + strand at 8.347 kb on - strand at 8.414 kb on - strand at 8.507 kb on - strand, within GFF1624 at 8.513 kb on - strand, within GFF1624 at 8.654 kb on + strand, within GFF1624 at 8.655 kb on - strand, within GFF1624 at 8.699 kb on - strand, within GFF1624 at 8.769 kb on - strand, within GFF1624 at 8.800 kb on + strand, within GFF1624 at 8.930 kb on - strand, within GFF1624 at 8.955 kb on - strand, within GFF1624 at 9.074 kb on + strand, within GFF1624 at 9.075 kb on - strand, within GFF1624
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 6,565 + GFF1621 0.39 +0.5 6,570 + GFF1621 0.41 -0.1 6,730 + +0.4 6,749 + -0.1 6,750 - -0.8 6,812 + +0.1 7,043 + GFF1622 0.35 -2.9 7,043 + GFF1622 0.35 -0.3 7,049 + GFF1622 0.36 +0.2 7,062 - GFF1622 0.38 -0.5 7,076 + GFF1622 0.40 -0.6 7,090 + GFF1622 0.42 -0.1 7,104 - GFF1622 0.44 -0.3 7,217 + GFF1622 0.61 -0.1 7,219 + GFF1622 0.62 -0.4 7,219 + GFF1622 0.62 -0.0 7,220 - GFF1622 0.62 +0.6 7,220 - GFF1622 0.62 -0.5 7,220 - GFF1622 0.62 -0.9 7,295 - GFF1622 0.73 -0.3 7,314 + GFF1622 0.76 -0.2 7,382 + GFF1622 0.87 -0.1 7,383 - GFF1622 0.87 -1.0 7,411 + -0.2 7,468 - +0.3 7,542 + -1.2 7,648 + GFF1623 0.22 +1.1 7,712 - GFF1623 0.31 +0.5 7,721 + GFF1623 0.33 -0.3 7,755 + GFF1623 0.37 +0.5 7,759 - GFF1623 0.38 +1.4 7,834 + GFF1623 0.49 -0.2 7,866 + GFF1623 0.53 -1.4 7,913 + GFF1623 0.60 +0.3 7,915 + GFF1623 0.60 +1.8 7,940 - GFF1623 0.64 -0.1 7,943 - GFF1623 0.64 -0.4 8,102 - GFF1623 0.87 +0.8 8,234 + +0.0 8,342 + +0.1 8,342 + -0.4 8,347 - -0.4 8,414 - -0.6 8,507 - GFF1624 0.14 -0.4 8,513 - GFF1624 0.14 +0.6 8,654 + GFF1624 0.21 -0.3 8,655 - GFF1624 0.21 -0.4 8,699 - GFF1624 0.23 -0.8 8,769 - GFF1624 0.26 -0.8 8,800 + GFF1624 0.28 -1.0 8,930 - GFF1624 0.34 -0.1 8,955 - GFF1624 0.36 -1.0 9,074 + GFF1624 0.41 -0.6 9,075 - GFF1624 0.41 -0.5
Or see this region's nucleotide sequence