Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1617 and GFF1618 are separated by 373 nucleotides GFF1618 and GFF1619 are separated by 16 nucleotides GFF1619 and GFF1620 are separated by 0 nucleotides GFF1620 and GFF1621 are separated by 137 nucleotides GFF1621 and GFF1622 are separated by 107 nucleotides
GFF1617 - Mobile element protein, at 3,489 to 4,343
GFF1617
GFF1618 - FIG00923183: possible membrane protein YebY, at 4,717 to 5,070
GFF1618
GFF1619 - Copper resistance protein D, at 5,087 to 5,962
GFF1619
GFF1620 - Copper resistance protein CopC, at 5,963 to 6,337
GFF1620
GFF1621 - DNA polymerase III theta subunit (EC 2.7.7.7), at 6,475 to 6,705
GFF1621
GFF1622 - Putative amidohydrolase, at 6,813 to 7,469
GFF1622
Position (kb) 5
6 Strain fitness (log2 ratio) -2
-1
0
1 at 4.184 kb on + strand, within GFF1617 at 4.850 kb on - strand, within GFF1618 at 4.893 kb on + strand, within GFF1618 at 4.893 kb on + strand, within GFF1618 at 4.910 kb on + strand, within GFF1618 at 4.910 kb on + strand, within GFF1618 at 4.911 kb on - strand, within GFF1618 at 4.994 kb on - strand, within GFF1618 at 4.994 kb on - strand, within GFF1618 at 5.132 kb on + strand at 5.132 kb on + strand at 5.206 kb on + strand, within GFF1619 at 5.207 kb on - strand, within GFF1619 at 5.251 kb on + strand, within GFF1619 at 5.251 kb on + strand, within GFF1619 at 5.251 kb on + strand, within GFF1619 at 5.252 kb on - strand, within GFF1619 at 5.252 kb on - strand, within GFF1619 at 5.293 kb on - strand, within GFF1619 at 5.331 kb on - strand, within GFF1619 at 5.407 kb on + strand, within GFF1619 at 5.410 kb on + strand, within GFF1619 at 5.636 kb on + strand, within GFF1619 at 5.847 kb on - strand, within GFF1619 at 5.884 kb on - strand at 5.884 kb on - strand at 5.898 kb on + strand at 5.915 kb on - strand at 5.965 kb on - strand at 5.982 kb on + strand at 5.983 kb on - strand at 5.998 kb on + strand at 5.998 kb on + strand at 6.030 kb on - strand, within GFF1620 at 6.086 kb on - strand, within GFF1620 at 6.358 kb on - strand at 6.439 kb on + strand at 6.439 kb on + strand at 6.443 kb on - strand at 6.475 kb on - strand at 6.565 kb on + strand, within GFF1621 at 6.570 kb on + strand, within GFF1621 at 6.730 kb on + strand at 6.749 kb on + strand at 6.750 kb on - strand at 6.812 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 4,184 + GFF1617 0.81 -0.8 4,850 - GFF1618 0.38 +0.6 4,893 + GFF1618 0.50 -0.5 4,893 + GFF1618 0.50 -0.1 4,910 + GFF1618 0.55 -1.1 4,910 + GFF1618 0.55 -0.4 4,911 - GFF1618 0.55 -0.8 4,994 - GFF1618 0.78 -0.4 4,994 - GFF1618 0.78 -0.9 5,132 + -0.7 5,132 + +0.3 5,206 + GFF1619 0.14 -0.1 5,207 - GFF1619 0.14 +0.3 5,251 + GFF1619 0.19 -0.4 5,251 + GFF1619 0.19 -0.2 5,251 + GFF1619 0.19 -0.8 5,252 - GFF1619 0.19 -1.1 5,252 - GFF1619 0.19 -1.0 5,293 - GFF1619 0.24 -1.0 5,331 - GFF1619 0.28 -0.9 5,407 + GFF1619 0.37 -1.9 5,410 + GFF1619 0.37 -0.9 5,636 + GFF1619 0.63 -0.9 5,847 - GFF1619 0.87 -0.7 5,884 - -1.1 5,884 - -2.3 5,898 + -0.1 5,915 - -1.8 5,965 - -1.1 5,982 + +0.1 5,983 - -0.5 5,998 + -1.2 5,998 + -1.7 6,030 - GFF1620 0.18 -0.3 6,086 - GFF1620 0.33 -1.1 6,358 - -0.1 6,439 + -0.2 6,439 + +1.3 6,443 - +0.1 6,475 - +0.8 6,565 + GFF1621 0.39 +0.5 6,570 + GFF1621 0.41 -0.1 6,730 + +0.4 6,749 + -0.1 6,750 - -0.8 6,812 + +0.1
Or see this region's nucleotide sequence