Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1560 and GFF1561 are separated by 14 nucleotides GFF1561 and GFF1562 are separated by 10 nucleotides GFF1562 and GFF1563 are separated by 163 nucleotides GFF1563 and GFF1564 are separated by 25 nucleotides
GFF1560 - Tricarboxylate transport protein TctC, at 22,772 to 23,749
GFF1560
GFF1561 - Tricarboxylate transport protein TctB, at 23,764 to 24,195
GFF1561
GFF1562 - Tricarboxylate transport membrane protein TctA, at 24,206 to 25,720
GFF1562
GFF1563 - hypothetical protein, at 25,884 to 25,997
GFF1563
GFF1564 - Carbon starvation induced protein CsiD, at 26,023 to 27,000
GFF1564
Position (kb) 24
25
26 Strain fitness (log2 ratio) -2
-1
0
1 at 23.248 kb on + strand, within GFF1560 at 23.543 kb on + strand, within GFF1560 at 23.544 kb on - strand, within GFF1560 at 23.574 kb on - strand, within GFF1560 at 23.688 kb on - strand at 23.705 kb on - strand at 23.851 kb on + strand, within GFF1561 at 23.852 kb on - strand, within GFF1561 at 23.978 kb on + strand, within GFF1561 at 24.007 kb on + strand, within GFF1561 at 24.007 kb on + strand, within GFF1561 at 24.139 kb on + strand, within GFF1561 at 24.171 kb on - strand at 24.171 kb on - strand at 24.394 kb on - strand, within GFF1562 at 24.413 kb on + strand, within GFF1562 at 24.413 kb on + strand, within GFF1562 at 24.414 kb on - strand, within GFF1562 at 24.414 kb on - strand, within GFF1562 at 24.429 kb on - strand, within GFF1562 at 24.782 kb on + strand, within GFF1562 at 24.867 kb on + strand, within GFF1562 at 25.042 kb on + strand, within GFF1562 at 25.046 kb on + strand, within GFF1562 at 25.046 kb on + strand, within GFF1562 at 25.047 kb on - strand, within GFF1562 at 25.048 kb on + strand, within GFF1562 at 25.049 kb on - strand, within GFF1562 at 25.421 kb on + strand, within GFF1562 at 25.422 kb on - strand, within GFF1562 at 25.422 kb on - strand, within GFF1562 at 25.492 kb on + strand, within GFF1562 at 25.591 kb on + strand at 25.741 kb on + strand at 25.741 kb on + strand at 25.741 kb on + strand at 25.742 kb on - strand at 25.755 kb on - strand at 25.805 kb on - strand at 26.077 kb on - strand at 26.210 kb on + strand, within GFF1564 at 26.210 kb on + strand, within GFF1564 at 26.210 kb on + strand, within GFF1564 at 26.210 kb on + strand, within GFF1564 at 26.258 kb on + strand, within GFF1564 at 26.402 kb on - strand, within GFF1564 at 26.422 kb on + strand, within GFF1564 at 26.467 kb on + strand, within GFF1564 at 26.512 kb on - strand, within GFF1564 at 26.515 kb on + strand, within GFF1564 at 26.516 kb on - strand, within GFF1564 at 26.537 kb on - strand, within GFF1564 at 26.537 kb on - strand, within GFF1564 at 26.569 kb on - strand, within GFF1564
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 23,248 + GFF1560 0.49 -0.1 23,543 + GFF1560 0.79 -0.4 23,544 - GFF1560 0.79 +0.3 23,574 - GFF1560 0.82 +1.4 23,688 - +0.5 23,705 - -0.6 23,851 + GFF1561 0.20 -0.4 23,852 - GFF1561 0.20 -2.0 23,978 + GFF1561 0.50 -0.1 24,007 + GFF1561 0.56 +0.4 24,007 + GFF1561 0.56 +0.0 24,139 + GFF1561 0.87 -0.7 24,171 - -0.7 24,171 - +0.7 24,394 - GFF1562 0.12 +0.6 24,413 + GFF1562 0.14 +0.0 24,413 + GFF1562 0.14 +0.5 24,414 - GFF1562 0.14 +0.5 24,414 - GFF1562 0.14 -0.1 24,429 - GFF1562 0.15 -1.6 24,782 + GFF1562 0.38 +0.4 24,867 + GFF1562 0.44 +1.1 25,042 + GFF1562 0.55 +0.1 25,046 + GFF1562 0.55 -0.2 25,046 + GFF1562 0.55 -0.6 25,047 - GFF1562 0.56 -0.6 25,048 + GFF1562 0.56 -0.1 25,049 - GFF1562 0.56 +0.2 25,421 + GFF1562 0.80 -0.4 25,422 - GFF1562 0.80 +0.3 25,422 - GFF1562 0.80 +0.4 25,492 + GFF1562 0.85 +0.2 25,591 + -0.7 25,741 + +0.2 25,741 + -0.1 25,741 + -0.6 25,742 - +0.9 25,755 - -0.4 25,805 - +0.2 26,077 - +1.3 26,210 + GFF1564 0.19 -1.6 26,210 + GFF1564 0.19 +1.2 26,210 + GFF1564 0.19 +0.5 26,210 + GFF1564 0.19 -0.6 26,258 + GFF1564 0.24 -0.5 26,402 - GFF1564 0.39 +1.4 26,422 + GFF1564 0.41 -0.7 26,467 + GFF1564 0.45 -0.1 26,512 - GFF1564 0.50 -1.0 26,515 + GFF1564 0.50 +0.3 26,516 - GFF1564 0.50 -0.9 26,537 - GFF1564 0.53 +0.9 26,537 - GFF1564 0.53 +0.6 26,569 - GFF1564 0.56 +0.9
Or see this region's nucleotide sequence