Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1422 and GFF1423 are separated by 185 nucleotides GFF1423 and GFF1424 are separated by 186 nucleotides
GFF1422 - Alanine racemase (EC 5.1.1.1), at 68,169 to 69,248
GFF1422
GFF1423 - Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57), at 69,434 to 70,627
GFF1423
GFF1424 - NMN phosphatase (EC 3.1.3.5); Class B acid phosphatase precursor (EC 3.1.3.2), at 70,814 to 71,527
GFF1424
Position (kb)
69
70
71 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 68.457 kb on - strand, within GFF1422 at 68.465 kb on - strand, within GFF1422 at 68.465 kb on - strand, within GFF1422 at 68.589 kb on + strand, within GFF1422 at 68.633 kb on + strand, within GFF1422 at 68.721 kb on - strand, within GFF1422 at 68.721 kb on - strand, within GFF1422 at 68.738 kb on + strand, within GFF1422 at 69.167 kb on + strand at 69.195 kb on + strand at 69.203 kb on + strand at 69.237 kb on + strand at 69.238 kb on - strand at 69.239 kb on + strand at 69.343 kb on - strand at 69.390 kb on + strand at 69.456 kb on - strand at 69.559 kb on + strand, within GFF1423 at 69.559 kb on + strand, within GFF1423 at 69.630 kb on - strand, within GFF1423 at 69.652 kb on + strand, within GFF1423 at 69.653 kb on - strand, within GFF1423 at 69.805 kb on - strand, within GFF1423 at 70.112 kb on + strand, within GFF1423 at 70.112 kb on + strand, within GFF1423 at 70.283 kb on + strand, within GFF1423 at 70.390 kb on + strand, within GFF1423 at 70.390 kb on + strand, within GFF1423 at 70.390 kb on + strand, within GFF1423 at 70.392 kb on + strand, within GFF1423 at 70.393 kb on - strand, within GFF1423 at 70.454 kb on + strand, within GFF1423 at 70.461 kb on + strand, within GFF1423 at 70.485 kb on - strand, within GFF1423 at 70.486 kb on + strand, within GFF1423 at 70.487 kb on - strand, within GFF1423 at 70.576 kb on + strand at 70.591 kb on - strand at 70.626 kb on - strand at 70.672 kb on - strand at 70.672 kb on - strand at 70.672 kb on - strand at 70.672 kb on - strand at 70.887 kb on + strand, within GFF1424 at 71.097 kb on - strand, within GFF1424 at 71.214 kb on - strand, within GFF1424 at 71.214 kb on - strand, within GFF1424 at 71.214 kb on - strand, within GFF1424 at 71.401 kb on + strand, within GFF1424 at 71.402 kb on - strand, within GFF1424 at 71.620 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 68,457 - GFF1422 0.27 +0.7 68,465 - GFF1422 0.27 +0.6 68,465 - GFF1422 0.27 -0.3 68,589 + GFF1422 0.39 +0.3 68,633 + GFF1422 0.43 +0.4 68,721 - GFF1422 0.51 +0.2 68,721 - GFF1422 0.51 +0.1 68,738 + GFF1422 0.53 +0.5 69,167 + -1.0 69,195 + -0.2 69,203 + -0.9 69,237 + +0.3 69,238 - +1.0 69,239 + +0.3 69,343 - +0.3 69,390 + +0.5 69,456 - -0.2 69,559 + GFF1423 0.10 -0.2 69,559 + GFF1423 0.10 -0.6 69,630 - GFF1423 0.16 -1.6 69,652 + GFF1423 0.18 -1.6 69,653 - GFF1423 0.18 -0.2 69,805 - GFF1423 0.31 -1.1 70,112 + GFF1423 0.57 -0.3 70,112 + GFF1423 0.57 -0.8 70,283 + GFF1423 0.71 -3.5 70,390 + GFF1423 0.80 -0.8 70,390 + GFF1423 0.80 -0.5 70,390 + GFF1423 0.80 -0.2 70,392 + GFF1423 0.80 +1.1 70,393 - GFF1423 0.80 -0.5 70,454 + GFF1423 0.85 -1.5 70,461 + GFF1423 0.86 -0.8 70,485 - GFF1423 0.88 -0.5 70,486 + GFF1423 0.88 -1.0 70,487 - GFF1423 0.88 +0.0 70,576 + -0.9 70,591 - +0.9 70,626 - -0.0 70,672 - -0.2 70,672 - -0.0 70,672 - +0.1 70,672 - +1.0 70,887 + GFF1424 0.10 -0.0 71,097 - GFF1424 0.40 -0.3 71,214 - GFF1424 0.56 +0.3 71,214 - GFF1424 0.56 -1.0 71,214 - GFF1424 0.56 +0.1 71,401 + GFF1424 0.82 -0.2 71,402 - GFF1424 0.82 +0.2 71,620 - -0.9
Or see this region's nucleotide sequence