Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF121

Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF120 and GFF121 are separated by 199 nucleotidesGFF121 and GFF122 are separated by 14 nucleotides GFF120 - Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase complex (EC 1.8.1.4) @ Dihydrolipoamide dehydrogenase (EC 1.8.1.4), at 106,659 to 108,083 GFF120 GFF121 - Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12), at 108,283 to 110,172 GFF121 GFF122 - Pyruvate dehydrogenase E1 component (EC 1.2.4.1), at 110,187 to 112,850 GFF122 Position (kb) 108 109 110 111Strain fitness (log2 ratio) -1 0 1 2 3 4at 108.284 kb on + strandat 108.285 kb on - strandat 108.315 kb on + strandat 108.315 kb on + strandat 108.448 kb on - strandat 108.448 kb on - strandat 108.529 kb on - strand, within GFF121at 108.529 kb on - strand, within GFF121at 108.807 kb on - strand, within GFF121at 109.189 kb on - strand, within GFF121at 109.962 kb on + strand, within GFF121at 109.962 kb on + strand, within GFF121at 109.962 kb on + strand, within GFF121at 109.963 kb on - strand, within GFF121at 110.308 kb on + strandat 110.309 kb on - strandat 110.412 kb on - strandat 110.440 kb on + strandat 110.448 kb on - strandat 110.579 kb on + strand, within GFF122at 110.580 kb on - strand, within GFF122at 110.582 kb on - strand, within GFF122at 110.584 kb on - strand, within GFF122at 110.584 kb on - strand, within GFF122at 110.584 kb on - strand, within GFF122at 110.584 kb on - strand, within GFF122at 110.676 kb on - strand, within GFF122at 110.712 kb on - strand, within GFF122at 110.762 kb on - strand, within GFF122at 110.786 kb on - strand, within GFF122at 110.789 kb on - strand, within GFF122at 110.815 kb on + strand, within GFF122at 110.816 kb on - strand, within GFF122at 110.820 kb on - strand, within GFF122at 110.820 kb on - strand, within GFF122at 110.820 kb on - strand, within GFF122at 110.820 kb on - strand, within GFF122at 110.820 kb on - strand, within GFF122at 110.876 kb on - strand, within GFF122at 110.955 kb on - strand, within GFF122at 111.047 kb on + strand, within GFF122at 111.048 kb on - strand, within GFF122at 111.048 kb on - strand, within GFF122at 111.048 kb on - strand, within GFF122at 111.048 kb on - strand, within GFF122at 111.052 kb on + strand, within GFF122at 111.052 kb on + strand, within GFF122at 111.055 kb on + strand, within GFF122at 111.056 kb on - strand, within GFF122at 111.062 kb on - strand, within GFF122at 111.094 kb on + strand, within GFF122

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
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108,284 + +2.4
108,285 - +2.5
108,315 + +2.1
108,315 + +2.6
108,448 - +2.8
108,448 - +2.2
108,529 - GFF121 0.13 +1.4
108,529 - GFF121 0.13 +2.5
108,807 - GFF121 0.28 +1.8
109,189 - GFF121 0.48 +1.1
109,962 + GFF121 0.89 +1.7
109,962 + GFF121 0.89 +1.0
109,962 + GFF121 0.89 +2.9
109,963 - GFF121 0.89 +1.8
110,308 + +2.0
110,309 - +2.3
110,412 - +1.0
110,440 + +1.1
110,448 - +2.7
110,579 + GFF122 0.15 +2.6
110,580 - GFF122 0.15 +1.0
110,582 - GFF122 0.15 +1.0
110,584 - GFF122 0.15 +3.1
110,584 - GFF122 0.15 +2.6
110,584 - GFF122 0.15 +1.3
110,584 - GFF122 0.15 +2.0
110,676 - GFF122 0.18 +2.7
110,712 - GFF122 0.20 +2.7
110,762 - GFF122 0.22 +1.8
110,786 - GFF122 0.22 +3.0
110,789 - GFF122 0.23 +2.1
110,815 + GFF122 0.24 +2.7
110,816 - GFF122 0.24 +2.1
110,820 - GFF122 0.24 +2.4
110,820 - GFF122 0.24 +2.7
110,820 - GFF122 0.24 +0.5
110,820 - GFF122 0.24 +2.5
110,820 - GFF122 0.24 +1.8
110,876 - GFF122 0.26 +2.5
110,955 - GFF122 0.29 +3.9
111,047 + GFF122 0.32 +4.0
111,048 - GFF122 0.32 +2.4
111,048 - GFF122 0.32 +1.7
111,048 - GFF122 0.32 +3.2
111,048 - GFF122 0.32 +2.0
111,052 + GFF122 0.32 +1.5
111,052 + GFF122 0.32 +2.1
111,055 + GFF122 0.33 +1.9
111,056 - GFF122 0.33 +1.7
111,062 - GFF122 0.33 +2.8
111,094 + GFF122 0.34 +4.1

Or see this region's nucleotide sequence