Experiment: L-Ornithine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0330 and Atu0331 are separated by 82 nucleotides Atu0331 and Atu0332 are separated by 233 nucleotides Atu0332 and Atu0333 are separated by 152 nucleotides Atu0333 and Atu0334 are separated by 9 nucleotides
Atu0330: Atu0330 - nitrogen regulatory IIA protein, at 323,822 to 324,283
Atu0330
Atu0331: Atu0331 - sigma-54 modulation protein, at 324,366 to 324,938
Atu0331
Atu0332: Atu0332 - RNA polymerase sigma-54 factor, at 325,172 to 326,716
Atu0332
Atu0333: Atu0333 - ABC transporter, nucleotide binding/ATPase protein, at 326,869 to 327,678
Atu0333
Atu0334: Atu0334 - hypothetical protein, at 327,688 to 328,251
Atu0334
Position (kb) 325
326
327 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 324.372 kb on - strand at 324.372 kb on - strand at 324.472 kb on - strand, within Atu0331 at 324.624 kb on - strand, within Atu0331 at 324.650 kb on - strand, within Atu0331 at 324.863 kb on + strand, within Atu0331 at 325.101 kb on + strand at 325.109 kb on - strand at 325.124 kb on + strand at 325.226 kb on + strand at 325.450 kb on - strand, within Atu0332 at 325.641 kb on - strand, within Atu0332 at 325.670 kb on - strand, within Atu0332 at 325.670 kb on - strand, within Atu0332 at 325.686 kb on + strand, within Atu0332 at 325.731 kb on + strand, within Atu0332 at 325.757 kb on + strand, within Atu0332 at 325.858 kb on - strand, within Atu0332 at 325.881 kb on - strand, within Atu0332 at 326.015 kb on - strand, within Atu0332 at 326.145 kb on - strand, within Atu0332 at 326.179 kb on + strand, within Atu0332 at 326.205 kb on - strand, within Atu0332 at 326.299 kb on - strand, within Atu0332 at 326.326 kb on - strand, within Atu0332 at 326.431 kb on + strand, within Atu0332 at 326.585 kb on - strand at 326.646 kb on - strand at 326.664 kb on + strand at 326.664 kb on - strand at 326.674 kb on - strand at 326.688 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Ornithine (N) remove 324,372 - -0.3 324,372 - -2.1 324,472 - Atu0331 0.18 -1.0 324,624 - Atu0331 0.45 -0.2 324,650 - Atu0331 0.50 -0.6 324,863 + Atu0331 0.87 -0.5 325,101 + +1.4 325,109 - -0.2 325,124 + -0.0 325,226 + -2.6 325,450 - Atu0332 0.18 -1.2 325,641 - Atu0332 0.30 +0.4 325,670 - Atu0332 0.32 -3.7 325,670 - Atu0332 0.32 -1.8 325,686 + Atu0332 0.33 -3.7 325,731 + Atu0332 0.36 -4.5 325,757 + Atu0332 0.38 -4.0 325,858 - Atu0332 0.44 -3.8 325,881 - Atu0332 0.46 -0.2 326,015 - Atu0332 0.55 -1.8 326,145 - Atu0332 0.63 -2.6 326,179 + Atu0332 0.65 -1.3 326,205 - Atu0332 0.67 -2.8 326,299 - Atu0332 0.73 +0.4 326,326 - Atu0332 0.75 -3.7 326,431 + Atu0332 0.81 -3.7 326,585 - -2.4 326,646 - -3.1 326,664 + -2.0 326,664 - -1.3 326,674 - -1.6 326,688 - -2.2
Or see this region's nucleotide sequence