Experiment: methylglyoxal 0.032 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06265 and PS417_06270 overlap by 4 nucleotides PS417_06270 and PS417_06275 are separated by 2 nucleotides PS417_06275 and PS417_06280 overlap by 4 nucleotides PS417_06280 and PS417_06285 are separated by 73 nucleotides
PS417_06265: PS417_06265 - 3-hydroxyacyl-ACP dehydratase, at 1,390,435 to 1,390,875
_06265
PS417_06270: PS417_06270 - UDP-N-acetylglucosamine acyltransferase, at 1,390,872 to 1,391,648
_06270
PS417_06275: PS417_06275 - lipid-A-disaccharide synthase, at 1,391,651 to 1,392,790
_06275
PS417_06280: PS417_06280 - ribonuclease HII, at 1,392,787 to 1,393,419
_06280
PS417_06285: PS417_06285 - DNA polymerase III subunit alpha, at 1,393,493 to 1,397,014
_06285
Position (kb) 1391
1392
1393 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1392.979 kb on + strand at 1393.268 kb on - strand, within PS417_06280 at 1393.268 kb on - strand, within PS417_06280 at 1393.418 kb on - strand at 1393.418 kb on - strand at 1393.479 kb on + strand at 1393.479 kb on + strand at 1393.479 kb on + strand at 1393.479 kb on + strand at 1393.479 kb on + strand at 1393.481 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.032 vol% remove 1,392,979 + +1.1 1,393,268 - PS417_06280 0.76 +2.0 1,393,268 - PS417_06280 0.76 +1.0 1,393,418 - +0.7 1,393,418 - -1.6 1,393,479 + +0.5 1,393,479 + -0.8 1,393,479 + -0.6 1,393,479 + -1.2 1,393,479 + -2.0 1,393,481 + -1.2
Or see this region's nucleotide sequence