Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0023 and Echvi_0024 are separated by 63 nucleotides Echvi_0024 and Echvi_0025 are separated by 6 nucleotides Echvi_0025 and Echvi_0026 are separated by 159 nucleotides
Echvi_0023: Echvi_0023 - phenylalanyl-tRNA synthetase, beta subunit, non-spirochete bacterial, at 21,000 to 23,411
_0023
Echvi_0024: Echvi_0024 - Protein of unknown function (DUF1684)., at 23,475 to 24,092
_0024
Echvi_0025: Echvi_0025 - DNA repair proteins, at 24,099 to 24,284
_0025
Echvi_0026: Echvi_0026 - Site-specific recombinase XerD, at 24,444 to 25,712
_0026
Position (kb) 24
25 Strain fitness (log2 ratio) -1
0
1
2
3 at 23.563 kb on + strand, within Echvi_0024 at 23.581 kb on + strand, within Echvi_0024 at 23.638 kb on + strand, within Echvi_0024 at 23.653 kb on + strand, within Echvi_0024 at 23.653 kb on + strand, within Echvi_0024 at 23.773 kb on + strand, within Echvi_0024 at 23.773 kb on + strand, within Echvi_0024 at 23.774 kb on - strand, within Echvi_0024 at 23.774 kb on - strand, within Echvi_0024 at 23.895 kb on + strand, within Echvi_0024 at 23.948 kb on - strand, within Echvi_0024 at 24.063 kb on + strand at 24.075 kb on + strand at 24.151 kb on - strand, within Echvi_0025 at 24.159 kb on + strand, within Echvi_0025 at 24.240 kb on - strand, within Echvi_0025 at 24.263 kb on + strand, within Echvi_0025 at 24.268 kb on - strand at 24.268 kb on - strand at 24.268 kb on - strand at 24.279 kb on + strand at 24.279 kb on + strand at 24.280 kb on - strand at 24.280 kb on - strand at 24.311 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 23,563 + Echvi_0024 0.14 +2.1 23,581 + Echvi_0024 0.17 +2.4 23,638 + Echvi_0024 0.26 +1.4 23,653 + Echvi_0024 0.29 +1.4 23,653 + Echvi_0024 0.29 +0.9 23,773 + Echvi_0024 0.48 +1.1 23,773 + Echvi_0024 0.48 +3.0 23,774 - Echvi_0024 0.48 -1.2 23,774 - Echvi_0024 0.48 +2.6 23,895 + Echvi_0024 0.68 +1.8 23,948 - Echvi_0024 0.77 +1.8 24,063 + +0.8 24,075 + +1.8 24,151 - Echvi_0025 0.28 -0.5 24,159 + Echvi_0025 0.32 -1.1 24,240 - Echvi_0025 0.76 +0.0 24,263 + Echvi_0025 0.88 -1.6 24,268 - +2.9 24,268 - +1.0 24,268 - +0.3 24,279 + +1.0 24,279 + +0.7 24,280 - -1.1 24,280 - +1.7 24,311 - -1.5
Or see this region's nucleotide sequence