Experiment: Gentamicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04110 and PS417_04115 are separated by 141 nucleotides PS417_04115 and PS417_04120 are separated by 24 nucleotides PS417_04120 and PS417_04125 are separated by 79 nucleotides
PS417_04110: PS417_04110 - cytochrome D ubiquinol oxidase subunit III, at 924,709 to 925,146
_04110
PS417_04115: PS417_04115 - alpha/beta hydrolase, at 925,288 to 925,923
_04115
PS417_04120: PS417_04120 - tryptophan--tRNA ligase, at 925,948 to 927,303
_04120
PS417_04125: PS417_04125 - ATPase, at 927,383 to 928,477
_04125
Position (kb)
925
926
927
928 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 925.203 kb on - strand at 925.203 kb on - strand at 925.245 kb on - strand at 925.426 kb on - strand, within PS417_04115 at 925.426 kb on - strand, within PS417_04115 at 925.433 kb on + strand, within PS417_04115 at 925.474 kb on - strand, within PS417_04115 at 925.524 kb on + strand, within PS417_04115 at 925.801 kb on + strand, within PS417_04115 at 925.935 kb on + strand at 927.479 kb on + strand at 927.550 kb on + strand, within PS417_04125 at 927.890 kb on + strand, within PS417_04125
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.008 mg/ml remove 925,203 - -0.3 925,203 - -3.3 925,245 - -0.5 925,426 - PS417_04115 0.22 -0.1 925,426 - PS417_04115 0.22 -0.5 925,433 + PS417_04115 0.23 -1.3 925,474 - PS417_04115 0.29 -2.0 925,524 + PS417_04115 0.37 -1.7 925,801 + PS417_04115 0.81 -1.3 925,935 + -1.4 927,479 + +2.1 927,550 + PS417_04125 0.15 +2.6 927,890 + PS417_04125 0.46 +2.3
Or see this region's nucleotide sequence