Experiment: Gentamicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_27500 and PS417_27505 are separated by 53 nucleotides PS417_27505 and PS417_27510 are separated by 209 nucleotides PS417_27510 and PS417_27515 are separated by 236 nucleotides
PS417_27500: PS417_27500 - peptidylprolyl isomerase, at 5,934,048 to 5,934,725
_27500
PS417_27505: PS417_27505 - transcriptional regulator, at 5,934,779 to 5,935,237
_27505
PS417_27510: PS417_27510 - disulfide bond formation protein B, at 5,935,447 to 5,935,968
_27510
PS417_27515: PS417_27515 - heme biosynthesis protein HemY, at 5,936,205 to 5,937,446
_27515
Position (kb) 5935
5936 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 5934.489 kb on + strand, within PS417_27500 at 5934.489 kb on + strand, within PS417_27500 at 5934.489 kb on + strand, within PS417_27500 at 5934.490 kb on - strand, within PS417_27500 at 5934.642 kb on + strand, within PS417_27500 at 5934.643 kb on - strand, within PS417_27500 at 5934.707 kb on - strand at 5934.707 kb on - strand at 5934.805 kb on + strand at 5934.805 kb on + strand at 5934.933 kb on - strand, within PS417_27505 at 5934.933 kb on - strand, within PS417_27505 at 5934.933 kb on - strand, within PS417_27505 at 5934.964 kb on - strand, within PS417_27505 at 5934.964 kb on - strand, within PS417_27505 at 5935.059 kb on + strand, within PS417_27505 at 5935.060 kb on - strand, within PS417_27505 at 5935.060 kb on - strand, within PS417_27505 at 5935.254 kb on + strand at 5935.254 kb on + strand at 5935.255 kb on - strand at 5935.360 kb on + strand at 5935.361 kb on - strand at 5935.361 kb on - strand at 5935.407 kb on + strand at 5935.418 kb on - strand at 5935.428 kb on + strand at 5935.649 kb on + strand, within PS417_27510 at 5935.649 kb on + strand, within PS417_27510 at 5935.650 kb on - strand, within PS417_27510 at 5935.650 kb on - strand, within PS417_27510 at 5935.763 kb on + strand, within PS417_27510 at 5935.764 kb on - strand, within PS417_27510 at 5935.893 kb on - strand, within PS417_27510 at 5935.991 kb on + strand at 5935.992 kb on - strand at 5936.030 kb on + strand at 5936.030 kb on + strand at 5936.030 kb on + strand at 5936.031 kb on - strand at 5936.031 kb on - strand at 5936.071 kb on - strand at 5936.130 kb on + strand at 5936.130 kb on + strand at 5936.131 kb on - strand at 5936.131 kb on - strand at 5936.193 kb on + strand at 5936.193 kb on + strand at 5936.194 kb on - strand at 5936.201 kb on + strand at 5936.202 kb on - strand at 5936.202 kb on - strand at 5936.521 kb on + strand, within PS417_27515 at 5936.521 kb on + strand, within PS417_27515 at 5936.522 kb on - strand, within PS417_27515 at 5936.620 kb on + strand, within PS417_27515 at 5936.621 kb on - strand, within PS417_27515 at 5936.792 kb on + strand, within PS417_27515
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.008 mg/ml remove 5,934,489 + PS417_27500 0.65 +0.1 5,934,489 + PS417_27500 0.65 +0.2 5,934,489 + PS417_27500 0.65 -0.3 5,934,490 - PS417_27500 0.65 -1.2 5,934,642 + PS417_27500 0.88 +0.8 5,934,643 - PS417_27500 0.88 -0.6 5,934,707 - -0.5 5,934,707 - -1.6 5,934,805 + -0.9 5,934,805 + -0.6 5,934,933 - PS417_27505 0.34 +1.0 5,934,933 - PS417_27505 0.34 +0.1 5,934,933 - PS417_27505 0.34 +1.1 5,934,964 - PS417_27505 0.40 -0.4 5,934,964 - PS417_27505 0.40 -2.4 5,935,059 + PS417_27505 0.61 +0.6 5,935,060 - PS417_27505 0.61 -0.7 5,935,060 - PS417_27505 0.61 -0.2 5,935,254 + +1.8 5,935,254 + -2.4 5,935,255 - -0.9 5,935,360 + -2.2 5,935,361 - +0.2 5,935,361 - +0.1 5,935,407 + -2.6 5,935,418 - +0.6 5,935,428 + -1.7 5,935,649 + PS417_27510 0.39 -1.1 5,935,649 + PS417_27510 0.39 -4.6 5,935,650 - PS417_27510 0.39 -1.9 5,935,650 - PS417_27510 0.39 -2.7 5,935,763 + PS417_27510 0.61 -2.3 5,935,764 - PS417_27510 0.61 -1.8 5,935,893 - PS417_27510 0.85 -2.6 5,935,991 + -2.9 5,935,992 - +4.8 5,936,030 + -1.4 5,936,030 + +0.3 5,936,030 + -2.4 5,936,031 - -1.4 5,936,031 - -2.0 5,936,071 - +1.8 5,936,130 + -0.2 5,936,130 + +0.6 5,936,131 - +2.8 5,936,131 - +2.4 5,936,193 + -1.0 5,936,193 + -1.3 5,936,194 - +3.2 5,936,201 + +0.5 5,936,202 - +3.3 5,936,202 - +2.7 5,936,521 + PS417_27515 0.25 -0.7 5,936,521 + PS417_27515 0.25 -1.4 5,936,522 - PS417_27515 0.26 -0.2 5,936,620 + PS417_27515 0.33 -0.4 5,936,621 - PS417_27515 0.33 +1.7 5,936,792 + PS417_27515 0.47 -3.6
Or see this region's nucleotide sequence