Experiment: Gentamicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24070 and PS417_24075 are separated by 86 nucleotides PS417_24075 and PS417_24080 are separated by 98 nucleotides PS417_24080 and PS417_24085 are separated by 63 nucleotides PS417_24085 and PS417_24090 overlap by 8 nucleotides PS417_24090 and PS417_24095 are separated by 168 nucleotides
PS417_24070: PS417_24070 - recombination and repair protein, at 5,212,762 to 5,214,435
_24070
PS417_24075: PS417_24075 - Fur family transcriptional regulator, at 5,214,522 to 5,214,926
_24075
PS417_24080: PS417_24080 - membrane protein, at 5,215,025 to 5,215,552
_24080
PS417_24085: PS417_24085 - protein RnfH, at 5,215,616 to 5,215,918
_24085
PS417_24090: PS417_24090 - ribosome association toxin RatA, at 5,215,911 to 5,216,345
_24090
PS417_24095: PS417_24095 - SsrA-binding protein, at 5,216,514 to 5,216,996
_24095
Position (kb) 5215
5216 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5214.102 kb on + strand, within PS417_24070 at 5214.102 kb on + strand, within PS417_24070 at 5214.103 kb on - strand, within PS417_24070 at 5214.210 kb on + strand, within PS417_24070 at 5214.210 kb on + strand, within PS417_24070 at 5214.210 kb on + strand, within PS417_24070 at 5214.211 kb on - strand, within PS417_24070 at 5214.221 kb on + strand, within PS417_24070 at 5214.221 kb on + strand, within PS417_24070 at 5214.222 kb on - strand, within PS417_24070 at 5214.443 kb on - strand at 5214.443 kb on - strand at 5214.454 kb on + strand at 5214.455 kb on - strand at 5214.456 kb on + strand at 5214.456 kb on + strand at 5214.456 kb on + strand at 5214.457 kb on - strand at 5214.457 kb on - strand at 5214.477 kb on + strand at 5214.478 kb on - strand at 5215.550 kb on + strand at 5215.617 kb on + strand at 5215.618 kb on - strand at 5215.618 kb on - strand at 5215.698 kb on + strand, within PS417_24085 at 5215.751 kb on + strand, within PS417_24085 at 5215.751 kb on + strand, within PS417_24085 at 5215.896 kb on + strand at 5215.896 kb on + strand at 5215.896 kb on + strand at 5215.896 kb on + strand at 5215.897 kb on - strand at 5215.897 kb on - strand at 5215.897 kb on - strand at 5216.499 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.008 mg/ml remove 5,214,102 + PS417_24070 0.80 -1.6 5,214,102 + PS417_24070 0.80 -0.6 5,214,103 - PS417_24070 0.80 -0.8 5,214,210 + PS417_24070 0.86 +1.3 5,214,210 + PS417_24070 0.86 -1.9 5,214,210 + PS417_24070 0.86 +0.3 5,214,211 - PS417_24070 0.87 -0.4 5,214,221 + PS417_24070 0.87 -2.0 5,214,221 + PS417_24070 0.87 +0.1 5,214,222 - PS417_24070 0.87 +2.0 5,214,443 - -1.5 5,214,443 - -0.6 5,214,454 + +0.2 5,214,455 - -3.2 5,214,456 + -0.5 5,214,456 + -0.3 5,214,456 + +0.5 5,214,457 - -1.0 5,214,457 - +0.2 5,214,477 + -1.3 5,214,478 - +0.3 5,215,550 + +0.9 5,215,617 + -0.2 5,215,618 - +0.8 5,215,618 - -0.6 5,215,698 + PS417_24085 0.27 +0.6 5,215,751 + PS417_24085 0.45 +1.6 5,215,751 + PS417_24085 0.45 +1.9 5,215,896 + +2.2 5,215,896 + +1.6 5,215,896 + +0.6 5,215,896 + +3.5 5,215,897 - +1.3 5,215,897 - +0.6 5,215,897 - +3.2 5,216,499 + -0.6
Or see this region's nucleotide sequence