Experiment: Gentamicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22950 and PS417_22955 are separated by 140 nucleotides PS417_22955 and PS417_22960 overlap by 4 nucleotides PS417_22960 and PS417_22965 are separated by 36 nucleotides PS417_22965 and PS417_22970 are separated by 149 nucleotides PS417_22970 and PS417_22975 are separated by 37 nucleotides
PS417_22950: PS417_22950 - preQ0 transporter, at 4,972,571 to 4,973,038
_22950
PS417_22955: PS417_22955 - Fe-S protein, at 4,973,179 to 4,973,397
_22955
PS417_22960: PS417_22960 - hypothetical protein, at 4,973,394 to 4,973,918
_22960
PS417_22965: PS417_22965 - DNA mismatch repair protein MutT, at 4,973,955 to 4,974,557
_22965
PS417_22970: PS417_22970 - ADP-ribose pyrophosphatase, at 4,974,707 to 4,975,255
_22970
PS417_22975: PS417_22975 - hypothetical protein, at 4,975,293 to 4,975,796
_22975
Position (kb) 4973
4974
4975 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4973.072 kb on + strand at 4973.073 kb on - strand at 4973.090 kb on - strand at 4973.208 kb on + strand, within PS417_22955 at 4973.305 kb on + strand, within PS417_22955 at 4973.331 kb on + strand, within PS417_22955 at 4973.331 kb on + strand, within PS417_22955 at 4973.331 kb on + strand at 4973.331 kb on + strand, within PS417_22955 at 4973.332 kb on - strand, within PS417_22955 at 4973.355 kb on + strand, within PS417_22955 at 4973.356 kb on - strand, within PS417_22955 at 4973.438 kb on + strand at 4973.438 kb on + strand at 4973.438 kb on + strand at 4973.446 kb on + strand at 4973.446 kb on + strand at 4973.447 kb on - strand, within PS417_22960 at 4973.447 kb on - strand, within PS417_22960 at 4973.641 kb on + strand, within PS417_22960 at 4973.642 kb on - strand, within PS417_22960 at 4973.912 kb on - strand at 4973.956 kb on + strand at 4973.956 kb on + strand at 4973.956 kb on + strand at 4973.956 kb on + strand at 4973.957 kb on - strand at 4973.957 kb on - strand at 4974.030 kb on + strand, within PS417_22965 at 4974.091 kb on + strand, within PS417_22965 at 4974.091 kb on + strand, within PS417_22965 at 4974.091 kb on + strand, within PS417_22965 at 4974.092 kb on - strand, within PS417_22965 at 4974.098 kb on + strand at 4974.099 kb on - strand, within PS417_22965 at 4974.099 kb on - strand, within PS417_22965 at 4974.099 kb on - strand, within PS417_22965 at 4974.099 kb on - strand, within PS417_22965 at 4974.203 kb on - strand, within PS417_22965 at 4974.212 kb on - strand, within PS417_22965 at 4974.312 kb on + strand, within PS417_22965 at 4974.313 kb on - strand, within PS417_22965 at 4974.543 kb on + strand at 4974.543 kb on + strand at 4974.543 kb on + strand at 4975.000 kb on + strand, within PS417_22970 at 4975.000 kb on + strand, within PS417_22970 at 4975.004 kb on + strand, within PS417_22970 at 4975.027 kb on + strand, within PS417_22970 at 4975.028 kb on - strand, within PS417_22970 at 4975.043 kb on - strand, within PS417_22970 at 4975.170 kb on + strand, within PS417_22970 at 4975.291 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.008 mg/ml remove 4,973,072 + +0.7 4,973,073 - +0.6 4,973,090 - +0.8 4,973,208 + PS417_22955 0.13 -0.4 4,973,305 + PS417_22955 0.58 -0.4 4,973,331 + PS417_22955 0.69 -2.5 4,973,331 + PS417_22955 0.69 -0.1 4,973,331 + +0.4 4,973,331 + PS417_22955 0.69 -2.7 4,973,332 - PS417_22955 0.70 +1.4 4,973,355 + PS417_22955 0.80 -0.9 4,973,356 - PS417_22955 0.81 -1.6 4,973,438 + -1.1 4,973,438 + +0.4 4,973,438 + +0.6 4,973,446 + -0.1 4,973,446 + -0.4 4,973,447 - PS417_22960 0.10 +0.0 4,973,447 - PS417_22960 0.10 +0.8 4,973,641 + PS417_22960 0.47 -0.9 4,973,642 - PS417_22960 0.47 +0.2 4,973,912 - -0.2 4,973,956 + +0.2 4,973,956 + +0.3 4,973,956 + -1.1 4,973,956 + +3.2 4,973,957 - -2.6 4,973,957 - +0.1 4,974,030 + PS417_22965 0.12 -0.9 4,974,091 + PS417_22965 0.23 -0.6 4,974,091 + PS417_22965 0.23 -1.9 4,974,091 + PS417_22965 0.23 -1.7 4,974,092 - PS417_22965 0.23 -0.2 4,974,098 + +0.4 4,974,099 - PS417_22965 0.24 -2.3 4,974,099 - PS417_22965 0.24 -0.9 4,974,099 - PS417_22965 0.24 -1.6 4,974,099 - PS417_22965 0.24 -0.1 4,974,203 - PS417_22965 0.41 -1.7 4,974,212 - PS417_22965 0.43 +1.1 4,974,312 + PS417_22965 0.59 +0.2 4,974,313 - PS417_22965 0.59 -1.4 4,974,543 + -1.1 4,974,543 + -1.9 4,974,543 + -2.5 4,975,000 + PS417_22970 0.53 -1.6 4,975,000 + PS417_22970 0.53 +1.0 4,975,004 + PS417_22970 0.54 -0.3 4,975,027 + PS417_22970 0.58 +2.7 4,975,028 - PS417_22970 0.58 +0.9 4,975,043 - PS417_22970 0.61 +0.1 4,975,170 + PS417_22970 0.84 -0.1 4,975,291 + -0.0
Or see this region's nucleotide sequence