Experiment: Gentamicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22715 and PS417_22720 are separated by 162 nucleotides PS417_22720 and PS417_22725 are separated by 139 nucleotides PS417_22725 and PS417_22730 are separated by 133 nucleotides PS417_22730 and PS417_22735 are separated by 139 nucleotides
PS417_22715: PS417_22715 - heme oxygenase, at 4,920,918 to 4,921,526
_22715
PS417_22720: PS417_22720 - membrane protein, at 4,921,689 to 4,922,090
_22720
PS417_22725: PS417_22725 - chemotaxis protein, at 4,922,230 to 4,924,368
_22725
PS417_22730: PS417_22730 - nitroreductase, at 4,924,502 to 4,925,065
_22730
PS417_22735: PS417_22735 - thiol oxidoreductase, at 4,925,205 to 4,926,632
_22735
Position (kb)
4922
4923
4924
4925 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4921.313 kb on - strand, within PS417_22715 at 4921.484 kb on - strand at 4921.584 kb on + strand at 4921.599 kb on - strand at 4921.644 kb on + strand at 4921.749 kb on + strand, within PS417_22720 at 4921.749 kb on + strand, within PS417_22720 at 4921.749 kb on + strand, within PS417_22720 at 4921.750 kb on - strand, within PS417_22720 at 4921.750 kb on - strand, within PS417_22720 at 4922.086 kb on + strand at 4922.087 kb on - strand at 4922.087 kb on - strand at 4922.087 kb on - strand at 4922.087 kb on - strand at 4922.101 kb on - strand at 4922.101 kb on - strand at 4922.101 kb on - strand at 4922.108 kb on - strand at 4922.168 kb on - strand at 4922.168 kb on - strand at 4922.193 kb on + strand at 4922.193 kb on + strand at 4922.194 kb on - strand at 4922.194 kb on - strand at 4922.194 kb on - strand at 4922.229 kb on + strand at 4922.229 kb on + strand at 4922.229 kb on + strand at 4922.229 kb on + strand at 4922.230 kb on - strand at 4922.698 kb on + strand, within PS417_22725 at 4922.699 kb on - strand, within PS417_22725 at 4922.837 kb on + strand, within PS417_22725 at 4922.838 kb on - strand, within PS417_22725 at 4923.122 kb on + strand, within PS417_22725 at 4923.123 kb on - strand, within PS417_22725 at 4923.123 kb on - strand, within PS417_22725 at 4923.458 kb on - strand, within PS417_22725 at 4923.780 kb on + strand, within PS417_22725 at 4923.781 kb on - strand, within PS417_22725 at 4924.482 kb on + strand at 4924.482 kb on + strand at 4924.483 kb on - strand at 4924.483 kb on - strand at 4924.546 kb on + strand at 4925.283 kb on - strand at 4925.283 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.008 mg/ml remove 4,921,313 - PS417_22715 0.65 +0.8 4,921,484 - -0.0 4,921,584 + +0.1 4,921,599 - -0.2 4,921,644 + +0.5 4,921,749 + PS417_22720 0.15 -0.4 4,921,749 + PS417_22720 0.15 +0.6 4,921,749 + PS417_22720 0.15 -1.0 4,921,750 - PS417_22720 0.15 -0.5 4,921,750 - PS417_22720 0.15 +0.8 4,922,086 + -3.3 4,922,087 - -0.6 4,922,087 - -1.2 4,922,087 - -2.0 4,922,087 - -2.6 4,922,101 - +0.6 4,922,101 - -0.6 4,922,101 - -0.9 4,922,108 - +0.4 4,922,168 - -0.0 4,922,168 - -0.2 4,922,193 + +3.4 4,922,193 + -0.7 4,922,194 - +0.8 4,922,194 - -1.1 4,922,194 - -0.8 4,922,229 + -0.2 4,922,229 + -0.1 4,922,229 + -1.7 4,922,229 + +2.2 4,922,230 - -1.9 4,922,698 + PS417_22725 0.22 +3.8 4,922,699 - PS417_22725 0.22 +1.3 4,922,837 + PS417_22725 0.28 -0.9 4,922,838 - PS417_22725 0.28 +0.4 4,923,122 + PS417_22725 0.42 +0.3 4,923,123 - PS417_22725 0.42 -2.0 4,923,123 - PS417_22725 0.42 +1.2 4,923,458 - PS417_22725 0.57 -0.3 4,923,780 + PS417_22725 0.72 +2.9 4,923,781 - PS417_22725 0.73 +1.1 4,924,482 + +0.7 4,924,482 + +1.0 4,924,483 - +1.0 4,924,483 - -0.5 4,924,546 + +0.7 4,925,283 - -0.9 4,925,283 - +1.0
Or see this region's nucleotide sequence