Experiment: Gentamicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_20850 and PS417_20855 are separated by 20 nucleotides PS417_20855 and PS417_20860 are separated by 453 nucleotides PS417_20860 and PS417_20865 are separated by 176 nucleotides
PS417_20850: PS417_20850 - hypothetical protein, at 4,527,009 to 4,528,970
_20850
PS417_20855: PS417_20855 - hypothetical protein, at 4,528,991 to 4,529,422
_20855
PS417_20860: PS417_20860 - DNA polymerase III subunit gamma/tau, at 4,529,876 to 4,531,039
_20860
PS417_20865: PS417_20865 - NAD-dependent DNA ligase LigA, at 4,531,216 to 4,533,573
_20865
Position (kb)
4529
4530
4531
4532 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4528.952 kb on + strand at 4528.971 kb on - strand at 4529.047 kb on + strand, within PS417_20855 at 4529.048 kb on - strand, within PS417_20855 at 4529.711 kb on - strand at 4529.773 kb on + strand at 4529.774 kb on - strand at 4529.774 kb on - strand at 4529.784 kb on - strand at 4529.932 kb on + strand at 4529.976 kb on + strand at 4530.087 kb on + strand, within PS417_20860 at 4530.283 kb on - strand, within PS417_20860 at 4530.306 kb on + strand, within PS417_20860 at 4530.306 kb on + strand, within PS417_20860 at 4530.382 kb on - strand, within PS417_20860 at 4530.385 kb on - strand, within PS417_20860
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.008 mg/ml remove 4,528,952 + -1.7 4,528,971 - -0.2 4,529,047 + PS417_20855 0.13 -3.8 4,529,048 - PS417_20855 0.13 -2.0 4,529,711 - -1.7 4,529,773 + -1.3 4,529,774 - +0.2 4,529,774 - -0.5 4,529,784 - -2.2 4,529,932 + +0.7 4,529,976 + -0.1 4,530,087 + PS417_20860 0.18 -1.4 4,530,283 - PS417_20860 0.35 -0.5 4,530,306 + PS417_20860 0.37 -3.4 4,530,306 + PS417_20860 0.37 -0.0 4,530,382 - PS417_20860 0.43 -0.5 4,530,385 - PS417_20860 0.44 -0.4
Or see this region's nucleotide sequence