Experiment: Gentamicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01425 and PS417_01430 are separated by 533 nucleotides PS417_01430 and PS417_01435 overlap by 4 nucleotides PS417_01435 and PS417_01440 overlap by 11 nucleotides
PS417_01425: PS417_01425 - AraC family transcriptional regulator, at 315,999 to 316,868
_01425
PS417_01430: PS417_01430 - chromosome segregation protein SMC, at 317,402 to 319,762
_01430
PS417_01435: PS417_01435 - hypothetical protein, at 319,759 to 320,478
_01435
PS417_01440: PS417_01440 - hypothetical protein, at 320,468 to 321,907
_01440
Position (kb) 317
318
319
320 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 316.595 kb on - strand, within PS417_01425 at 316.851 kb on + strand at 316.852 kb on - strand at 316.852 kb on - strand at 316.852 kb on - strand at 316.998 kb on + strand at 316.998 kb on + strand at 316.999 kb on - strand at 316.999 kb on - strand at 317.022 kb on + strand at 317.022 kb on + strand at 317.023 kb on - strand at 317.023 kb on - strand at 317.101 kb on + strand at 317.146 kb on - strand at 317.155 kb on + strand at 317.156 kb on - strand at 317.158 kb on + strand at 317.158 kb on + strand at 317.158 kb on + strand at 317.159 kb on - strand at 317.159 kb on - strand at 317.177 kb on + strand at 317.227 kb on - strand at 317.291 kb on + strand at 317.292 kb on - strand at 317.292 kb on - strand at 317.707 kb on + strand, within PS417_01430 at 318.308 kb on - strand, within PS417_01430 at 318.897 kb on + strand, within PS417_01430 at 318.998 kb on + strand, within PS417_01430 at 319.299 kb on - strand, within PS417_01430 at 319.475 kb on + strand, within PS417_01430 at 319.555 kb on + strand at 319.555 kb on + strand at 319.556 kb on - strand at 319.844 kb on + strand, within PS417_01435
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.008 mg/ml remove 316,595 - PS417_01425 0.69 +1.1 316,851 + +0.0 316,852 - -1.2 316,852 - +0.7 316,852 - -2.3 316,998 + -2.9 316,998 + +0.5 316,999 - +0.6 316,999 - -2.2 317,022 + +0.9 317,022 + +0.8 317,023 - +0.1 317,023 - +0.9 317,101 + -0.1 317,146 - -0.0 317,155 + -1.1 317,156 - +1.1 317,158 + -0.0 317,158 + -1.7 317,158 + -0.1 317,159 - -2.3 317,159 - +1.2 317,177 + -1.2 317,227 - -1.7 317,291 + +1.3 317,292 - -0.9 317,292 - -0.5 317,707 + PS417_01430 0.13 +2.2 318,308 - PS417_01430 0.38 +1.2 318,897 + PS417_01430 0.63 -0.1 318,998 + PS417_01430 0.68 -1.5 319,299 - PS417_01430 0.80 -0.2 319,475 + PS417_01430 0.88 +1.2 319,555 + -0.7 319,555 + +1.2 319,556 - -0.2 319,844 + PS417_01435 0.12 -0.1
Or see this region's nucleotide sequence