Experiment: Gentamicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13835 and PS417_13840 are separated by 3 nucleotides PS417_13840 and PS417_13845 are separated by 61 nucleotides PS417_13845 and PS417_13850 are separated by 11 nucleotides
PS417_13835: PS417_13835 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) (from data) , at 3,039,345 to 3,040,448
_13835
PS417_13840: PS417_13840 - isobutyryl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 3,040,452 to 3,041,603
_13840
PS417_13845: PS417_13845 - enoyl-CoA hydratase, at 3,041,665 to 3,042,438
_13845
PS417_13850: PS417_13850 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 3,042,450 to 3,043,577
_13850
Position (kb)
3040
3041
3042 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3039.538 kb on + strand at 3039.539 kb on - strand, within PS417_13835 at 3039.808 kb on - strand, within PS417_13835 at 3039.831 kb on + strand, within PS417_13835 at 3039.832 kb on - strand, within PS417_13835 at 3039.867 kb on + strand, within PS417_13835 at 3039.867 kb on + strand, within PS417_13835 at 3039.867 kb on + strand, within PS417_13835 at 3039.868 kb on - strand, within PS417_13835 at 3039.868 kb on - strand, within PS417_13835 at 3039.933 kb on + strand, within PS417_13835 at 3039.933 kb on + strand, within PS417_13835 at 3040.235 kb on - strand, within PS417_13835 at 3040.615 kb on + strand, within PS417_13840 at 3040.616 kb on - strand, within PS417_13840 at 3040.642 kb on - strand, within PS417_13840 at 3040.651 kb on - strand, within PS417_13840 at 3040.725 kb on - strand, within PS417_13840 at 3041.481 kb on + strand, within PS417_13840 at 3041.482 kb on - strand, within PS417_13840 at 3041.482 kb on - strand, within PS417_13840 at 3041.614 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.008 mg/ml remove 3,039,538 + -0.9 3,039,539 - PS417_13835 0.18 +1.1 3,039,808 - PS417_13835 0.42 -0.5 3,039,831 + PS417_13835 0.44 +0.7 3,039,832 - PS417_13835 0.44 +0.3 3,039,867 + PS417_13835 0.47 -0.5 3,039,867 + PS417_13835 0.47 +0.2 3,039,867 + PS417_13835 0.47 -0.4 3,039,868 - PS417_13835 0.47 -0.4 3,039,868 - PS417_13835 0.47 -0.7 3,039,933 + PS417_13835 0.53 +2.2 3,039,933 + PS417_13835 0.53 +0.2 3,040,235 - PS417_13835 0.81 -0.1 3,040,615 + PS417_13840 0.14 +1.5 3,040,616 - PS417_13840 0.14 +1.1 3,040,642 - PS417_13840 0.16 -3.4 3,040,651 - PS417_13840 0.17 +1.3 3,040,725 - PS417_13840 0.24 -0.3 3,041,481 + PS417_13840 0.89 +0.4 3,041,482 - PS417_13840 0.89 -0.3 3,041,482 - PS417_13840 0.89 +0.7 3,041,614 + +0.9
Or see this region's nucleotide sequence