Experiment: Gentamicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12930 and PS417_12935 are separated by 292 nucleotides PS417_12935 and PS417_12940 overlap by 4 nucleotides PS417_12940 and PS417_12945 are separated by 37 nucleotides PS417_12945 and PS417_12950 overlap by 4 nucleotides
PS417_12930: PS417_12930 - LpxA family transferase, at 2,827,844 to 2,828,473
_12930
PS417_12935: PS417_12935 - RNA polymerase sigma factor, at 2,828,766 to 2,829,284
_12935
PS417_12940: PS417_12940 - sugar ABC transporter substrate-binding protein, at 2,829,281 to 2,830,207
_12940
PS417_12945: PS417_12945 - sulfonate ABC transporter ATP-binding protein, at 2,830,245 to 2,830,961
_12945
PS417_12950: PS417_12950 - ABC transporter permease, at 2,830,958 to 2,831,779
_12950
Position (kb)
2829
2830
2831 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2828.465 kb on + strand at 2828.466 kb on - strand at 2828.466 kb on - strand at 2828.537 kb on + strand at 2828.606 kb on - strand at 2828.631 kb on + strand at 2828.669 kb on + strand at 2828.669 kb on + strand at 2828.780 kb on - strand at 2828.780 kb on - strand at 2828.964 kb on + strand, within PS417_12935 at 2829.031 kb on - strand, within PS417_12935 at 2829.031 kb on - strand, within PS417_12935 at 2829.240 kb on + strand at 2829.240 kb on + strand at 2829.240 kb on + strand at 2829.240 kb on + strand at 2829.242 kb on + strand at 2829.242 kb on + strand at 2829.243 kb on - strand at 2829.255 kb on + strand at 2829.804 kb on + strand, within PS417_12940 at 2829.951 kb on + strand, within PS417_12940 at 2829.951 kb on + strand, within PS417_12940 at 2830.048 kb on - strand, within PS417_12940 at 2830.048 kb on - strand, within PS417_12940 at 2830.170 kb on - strand at 2830.250 kb on + strand at 2830.402 kb on + strand, within PS417_12945 at 2830.672 kb on + strand, within PS417_12945 at 2830.672 kb on + strand, within PS417_12945 at 2830.929 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.008 mg/ml remove 2,828,465 + -1.2 2,828,466 - +1.0 2,828,466 - +0.2 2,828,537 + +0.1 2,828,606 - -0.9 2,828,631 + +4.4 2,828,669 + +0.1 2,828,669 + +0.9 2,828,780 - +1.1 2,828,780 - -1.7 2,828,964 + PS417_12935 0.38 +1.1 2,829,031 - PS417_12935 0.51 -0.7 2,829,031 - PS417_12935 0.51 +0.4 2,829,240 + +0.3 2,829,240 + -2.5 2,829,240 + -0.7 2,829,240 + +0.6 2,829,242 + +0.2 2,829,242 + +0.9 2,829,243 - -0.3 2,829,255 + -0.5 2,829,804 + PS417_12940 0.56 +1.6 2,829,951 + PS417_12940 0.72 +0.3 2,829,951 + PS417_12940 0.72 +0.4 2,830,048 - PS417_12940 0.83 +1.4 2,830,048 - PS417_12940 0.83 -0.4 2,830,170 - +1.6 2,830,250 + +0.6 2,830,402 + PS417_12945 0.22 +0.6 2,830,672 + PS417_12945 0.60 -1.0 2,830,672 + PS417_12945 0.60 -0.5 2,830,929 + +0.2
Or see this region's nucleotide sequence