Experiment: Gentamicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11870 and PS417_11875 are separated by 19 nucleotides PS417_11875 and PS417_11880 are separated by 155 nucleotides
PS417_11870: PS417_11870 - 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase IolD (EC 3.7.1.22) (from data) , at 2,583,734 to 2,585,665
_11870
PS417_11875: PS417_11875 - Inositol 2-dehydrogenase (EC 1.1.1.18) (from data) , at 2,585,685 to 2,586,695
_11875
PS417_11880: PS417_11880 - oxidoreductase, at 2,586,851 to 2,587,879
_11880
Position (kb)
2585
2586
2587 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2584.811 kb on + strand, within PS417_11870 at 2585.004 kb on + strand, within PS417_11870 at 2585.005 kb on - strand, within PS417_11870 at 2585.105 kb on + strand, within PS417_11870 at 2585.106 kb on - strand, within PS417_11870 at 2585.193 kb on - strand, within PS417_11870 at 2585.216 kb on + strand, within PS417_11870 at 2585.216 kb on + strand, within PS417_11870 at 2585.216 kb on + strand, within PS417_11870 at 2585.216 kb on + strand, within PS417_11870 at 2585.290 kb on + strand, within PS417_11870 at 2585.290 kb on + strand, within PS417_11870 at 2585.333 kb on - strand, within PS417_11870 at 2585.497 kb on + strand at 2585.663 kb on + strand at 2585.663 kb on + strand at 2585.684 kb on + strand at 2586.174 kb on + strand, within PS417_11875 at 2586.174 kb on + strand, within PS417_11875 at 2586.175 kb on - strand, within PS417_11875 at 2586.249 kb on + strand, within PS417_11875 at 2586.249 kb on + strand, within PS417_11875 at 2586.249 kb on + strand, within PS417_11875 at 2586.381 kb on + strand, within PS417_11875 at 2586.522 kb on + strand, within PS417_11875 at 2586.626 kb on + strand at 2586.626 kb on + strand at 2586.627 kb on - strand at 2586.687 kb on + strand at 2586.775 kb on - strand at 2586.846 kb on + strand at 2587.413 kb on + strand, within PS417_11880 at 2587.431 kb on - strand, within PS417_11880
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.008 mg/ml remove 2,584,811 + PS417_11870 0.56 -1.2 2,585,004 + PS417_11870 0.66 -2.3 2,585,005 - PS417_11870 0.66 -0.4 2,585,105 + PS417_11870 0.71 -1.3 2,585,106 - PS417_11870 0.71 -0.7 2,585,193 - PS417_11870 0.76 +0.0 2,585,216 + PS417_11870 0.77 -0.4 2,585,216 + PS417_11870 0.77 -0.9 2,585,216 + PS417_11870 0.77 -0.1 2,585,216 + PS417_11870 0.77 -0.7 2,585,290 + PS417_11870 0.81 -3.1 2,585,290 + PS417_11870 0.81 -1.4 2,585,333 - PS417_11870 0.83 +0.0 2,585,497 + -1.1 2,585,663 + -1.3 2,585,663 + -0.3 2,585,684 + -1.2 2,586,174 + PS417_11875 0.48 -0.5 2,586,174 + PS417_11875 0.48 -1.0 2,586,175 - PS417_11875 0.48 -0.5 2,586,249 + PS417_11875 0.56 -1.8 2,586,249 + PS417_11875 0.56 -0.8 2,586,249 + PS417_11875 0.56 +1.4 2,586,381 + PS417_11875 0.69 -0.9 2,586,522 + PS417_11875 0.83 -2.1 2,586,626 + -1.1 2,586,626 + -1.7 2,586,627 - -1.4 2,586,687 + -0.1 2,586,775 - +0.7 2,586,846 + -0.2 2,587,413 + PS417_11880 0.55 -1.6 2,587,431 - PS417_11880 0.56 +1.2
Or see this region's nucleotide sequence