Experiment: Gentamicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11010 and PS417_11015 are separated by 10 nucleotides PS417_11015 and PS417_11020 are separated by 36 nucleotides PS417_11020 and PS417_11025 are separated by 37 nucleotides
PS417_11010: PS417_11010 - FAH family protein, at 2,359,549 to 2,360,538
_11010
PS417_11015: PS417_11015 - Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26) (from data) , at 2,360,549 to 2,362,129
_11015
PS417_11020: PS417_11020 - L-arabinolactonase (EC 3.1.1.15) (from data) , at 2,362,166 to 2,363,029
_11020
PS417_11025: PS417_11025 - sugar ABC transporter substrate-binding protein, at 2,363,067 to 2,364,071
_11025
Position (kb)
2360
2361
2362
2363 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2359.571 kb on + strand at 2359.572 kb on - strand at 2359.572 kb on - strand at 2359.792 kb on - strand, within PS417_11010 at 2359.963 kb on + strand, within PS417_11010 at 2359.963 kb on + strand, within PS417_11010 at 2360.149 kb on + strand, within PS417_11010 at 2360.155 kb on + strand, within PS417_11010 at 2360.156 kb on - strand, within PS417_11010 at 2360.302 kb on - strand, within PS417_11010 at 2360.330 kb on - strand, within PS417_11010 at 2360.372 kb on + strand, within PS417_11010 at 2360.373 kb on - strand, within PS417_11010 at 2360.373 kb on - strand, within PS417_11010 at 2360.573 kb on + strand at 2360.591 kb on - strand at 2361.681 kb on + strand, within PS417_11015 at 2361.681 kb on + strand, within PS417_11015 at 2361.682 kb on - strand, within PS417_11015 at 2361.682 kb on - strand, within PS417_11015 at 2361.682 kb on - strand, within PS417_11015 at 2362.043 kb on - strand at 2362.174 kb on + strand at 2362.569 kb on + strand, within PS417_11020 at 2362.791 kb on - strand, within PS417_11020 at 2362.791 kb on - strand, within PS417_11020 at 2363.041 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.008 mg/ml remove 2,359,571 + -0.1 2,359,572 - -2.0 2,359,572 - +2.8 2,359,792 - PS417_11010 0.25 -0.8 2,359,963 + PS417_11010 0.42 -2.2 2,359,963 + PS417_11010 0.42 -0.8 2,360,149 + PS417_11010 0.61 -0.3 2,360,155 + PS417_11010 0.61 +0.4 2,360,156 - PS417_11010 0.61 -0.4 2,360,302 - PS417_11010 0.76 +0.2 2,360,330 - PS417_11010 0.79 +1.2 2,360,372 + PS417_11010 0.83 +2.3 2,360,373 - PS417_11010 0.83 +1.2 2,360,373 - PS417_11010 0.83 +0.2 2,360,573 + -1.4 2,360,591 - +0.4 2,361,681 + PS417_11015 0.72 +1.7 2,361,681 + PS417_11015 0.72 +0.6 2,361,682 - PS417_11015 0.72 -0.0 2,361,682 - PS417_11015 0.72 +1.0 2,361,682 - PS417_11015 0.72 +0.7 2,362,043 - -1.2 2,362,174 + -0.2 2,362,569 + PS417_11020 0.47 -0.1 2,362,791 - PS417_11020 0.72 +0.5 2,362,791 - PS417_11020 0.72 +0.3 2,363,041 + -0.4
Or see this region's nucleotide sequence