Experiment: Gentamicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10075 and PS417_10080 overlap by 4 nucleotides PS417_10080 and PS417_10085 overlap by 26 nucleotides
PS417_10075: PS417_10075 - terminase, at 2,161,058 to 2,162,359
_10075
PS417_10080: PS417_10080 - hypothetical protein, at 2,162,356 to 2,163,774
_10080
PS417_10085: PS417_10085 - head morphogenesis protein, at 2,163,749 to 2,164,837
_10085
Position (kb)
2162
2163
2164 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2161.526 kb on - strand, within PS417_10075 at 2161.604 kb on + strand, within PS417_10075 at 2161.604 kb on + strand, within PS417_10075 at 2161.604 kb on + strand, within PS417_10075 at 2161.658 kb on + strand, within PS417_10075 at 2161.658 kb on + strand, within PS417_10075 at 2161.706 kb on + strand, within PS417_10075 at 2161.706 kb on + strand, within PS417_10075 at 2161.748 kb on + strand, within PS417_10075 at 2161.749 kb on - strand, within PS417_10075 at 2161.749 kb on - strand, within PS417_10075 at 2161.757 kb on + strand, within PS417_10075 at 2162.202 kb on - strand, within PS417_10075 at 2162.291 kb on + strand at 2162.580 kb on - strand, within PS417_10080 at 2162.627 kb on - strand, within PS417_10080 at 2162.665 kb on + strand, within PS417_10080 at 2162.722 kb on + strand, within PS417_10080 at 2162.723 kb on - strand, within PS417_10080 at 2162.731 kb on + strand, within PS417_10080 at 2162.732 kb on - strand, within PS417_10080 at 2162.812 kb on - strand, within PS417_10080 at 2162.812 kb on - strand, within PS417_10080 at 2162.832 kb on - strand, within PS417_10080 at 2162.953 kb on - strand, within PS417_10080 at 2162.953 kb on - strand, within PS417_10080 at 2163.094 kb on + strand, within PS417_10080 at 2163.095 kb on - strand, within PS417_10080 at 2163.221 kb on - strand, within PS417_10080 at 2163.235 kb on + strand, within PS417_10080 at 2163.473 kb on - strand, within PS417_10080 at 2163.473 kb on - strand at 2163.473 kb on - strand, within PS417_10080 at 2163.473 kb on - strand, within PS417_10080 at 2163.502 kb on + strand, within PS417_10080 at 2163.502 kb on + strand, within PS417_10080 at 2163.640 kb on + strand at 2163.640 kb on + strand at 2163.971 kb on + strand, within PS417_10085 at 2163.971 kb on + strand, within PS417_10085 at 2163.985 kb on - strand, within PS417_10085 at 2164.087 kb on + strand, within PS417_10085 at 2164.087 kb on + strand, within PS417_10085 at 2164.088 kb on - strand, within PS417_10085 at 2164.214 kb on - strand, within PS417_10085 at 2164.214 kb on - strand, within PS417_10085 at 2164.214 kb on - strand, within PS417_10085 at 2164.217 kb on - strand, within PS417_10085 at 2164.217 kb on - strand, within PS417_10085 at 2164.597 kb on - strand, within PS417_10085 at 2164.638 kb on - strand, within PS417_10085 at 2164.640 kb on + strand, within PS417_10085 at 2164.641 kb on - strand, within PS417_10085
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.008 mg/ml remove 2,161,526 - PS417_10075 0.36 -0.4 2,161,604 + PS417_10075 0.42 -2.0 2,161,604 + PS417_10075 0.42 -2.3 2,161,604 + PS417_10075 0.42 +0.3 2,161,658 + PS417_10075 0.46 +1.1 2,161,658 + PS417_10075 0.46 +0.2 2,161,706 + PS417_10075 0.50 -0.7 2,161,706 + PS417_10075 0.50 +0.4 2,161,748 + PS417_10075 0.53 -0.5 2,161,749 - PS417_10075 0.53 -0.4 2,161,749 - PS417_10075 0.53 -0.1 2,161,757 + PS417_10075 0.54 -0.0 2,162,202 - PS417_10075 0.88 -0.6 2,162,291 + -0.4 2,162,580 - PS417_10080 0.16 +1.2 2,162,627 - PS417_10080 0.19 -0.3 2,162,665 + PS417_10080 0.22 +0.8 2,162,722 + PS417_10080 0.26 -0.0 2,162,723 - PS417_10080 0.26 +0.7 2,162,731 + PS417_10080 0.26 +1.0 2,162,732 - PS417_10080 0.26 +0.2 2,162,812 - PS417_10080 0.32 +0.8 2,162,812 - PS417_10080 0.32 -0.4 2,162,832 - PS417_10080 0.34 -0.5 2,162,953 - PS417_10080 0.42 +0.5 2,162,953 - PS417_10080 0.42 +1.8 2,163,094 + PS417_10080 0.52 +0.6 2,163,095 - PS417_10080 0.52 -0.3 2,163,221 - PS417_10080 0.61 -1.7 2,163,235 + PS417_10080 0.62 -0.2 2,163,473 - PS417_10080 0.79 +0.2 2,163,473 - +1.2 2,163,473 - PS417_10080 0.79 +0.1 2,163,473 - PS417_10080 0.79 +0.7 2,163,502 + PS417_10080 0.81 +0.0 2,163,502 + PS417_10080 0.81 -0.3 2,163,640 + -0.4 2,163,640 + +0.7 2,163,971 + PS417_10085 0.20 +0.9 2,163,971 + PS417_10085 0.20 +0.2 2,163,985 - PS417_10085 0.22 +0.3 2,164,087 + PS417_10085 0.31 +1.5 2,164,087 + PS417_10085 0.31 -1.1 2,164,088 - PS417_10085 0.31 +0.2 2,164,214 - PS417_10085 0.43 -0.1 2,164,214 - PS417_10085 0.43 +0.2 2,164,214 - PS417_10085 0.43 +0.6 2,164,217 - PS417_10085 0.43 -1.2 2,164,217 - PS417_10085 0.43 +0.5 2,164,597 - PS417_10085 0.78 +2.2 2,164,638 - PS417_10085 0.82 +0.1 2,164,640 + PS417_10085 0.82 -1.1 2,164,641 - PS417_10085 0.82 +0.2
Or see this region's nucleotide sequence