Experiment: Gentamicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09375 and PS417_09380 overlap by 4 nucleotides PS417_09380 and PS417_09385 overlap by 4 nucleotides PS417_09385 and PS417_09390 are separated by 19 nucleotides PS417_09390 and PS417_09395 overlap by 4 nucleotides PS417_09395 and PS417_09400 overlap by 4 nucleotides
PS417_09375: PS417_09375 - zinc ABC transporter permease, at 2,033,503 to 2,034,402
_09375
PS417_09380: PS417_09380 - manganese ABC transporter ATP-binding protein, at 2,034,399 to 2,035,145
_09380
PS417_09385: PS417_09385 - ABC transporter substrate-binding protein, at 2,035,142 to 2,036,062
_09385
PS417_09390: PS417_09390 - hypothetical protein, at 2,036,082 to 2,036,642
_09390
PS417_09395: PS417_09395 - hypothetical protein, at 2,036,639 to 2,036,968
_09395
PS417_09400: PS417_09400 - thiamine pyrophosphate-binding protein, at 2,036,965 to 2,037,501
_09400
Position (kb) 2035
2036
2037 Strain fitness (log2 ratio) -1
0
1
2 at 2034.649 kb on - strand, within PS417_09380 at 2035.566 kb on + strand, within PS417_09385 at 2035.933 kb on - strand, within PS417_09385 at 2035.939 kb on - strand, within PS417_09385 at 2036.591 kb on - strand at 2036.909 kb on - strand, within PS417_09395
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.008 mg/ml remove 2,034,649 - PS417_09380 0.33 +2.4 2,035,566 + PS417_09385 0.46 +0.2 2,035,933 - PS417_09385 0.86 -0.2 2,035,939 - PS417_09385 0.87 -1.1 2,036,591 - +0.9 2,036,909 - PS417_09395 0.82 +1.3
Or see this region's nucleotide sequence