Experiment: Gentamicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08145 and PS417_08150 are separated by 162 nucleotides PS417_08150 and PS417_08155 are separated by 132 nucleotides PS417_08155 and PS417_08160 are separated by 20 nucleotides
PS417_08145: PS417_08145 - 30S ribosomal protein S1, at 1,784,325 to 1,786,010
_08145
PS417_08150: PS417_08150 - hypothetical protein, at 1,786,173 to 1,786,457
_08150
PS417_08155: PS417_08155 - integration host factor subunit beta, at 1,786,590 to 1,786,886
_08155
PS417_08160: PS417_08160 - hypothetical protein, at 1,786,907 to 1,787,137
_08160
Position (kb)
1786
1787 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7
8 at 1786.097 kb on + strand at 1786.098 kb on - strand at 1786.099 kb on + strand at 1786.099 kb on + strand at 1786.100 kb on - strand at 1786.100 kb on - strand at 1786.100 kb on - strand at 1786.120 kb on + strand at 1786.121 kb on - strand at 1786.122 kb on + strand at 1786.123 kb on - strand at 1786.162 kb on + strand at 1786.185 kb on - strand at 1786.189 kb on + strand at 1786.189 kb on + strand at 1786.190 kb on - strand at 1786.348 kb on - strand, within PS417_08150 at 1786.396 kb on - strand, within PS417_08150 at 1786.396 kb on - strand, within PS417_08150 at 1786.426 kb on + strand, within PS417_08150 at 1786.451 kb on + strand at 1786.452 kb on - strand at 1786.472 kb on + strand at 1786.474 kb on + strand at 1786.474 kb on + strand at 1786.474 kb on + strand at 1786.474 kb on + strand at 1786.501 kb on - strand at 1786.540 kb on + strand at 1786.664 kb on + strand, within PS417_08155 at 1786.673 kb on + strand, within PS417_08155 at 1786.674 kb on - strand, within PS417_08155 at 1786.906 kb on + strand at 1786.938 kb on + strand, within PS417_08160 at 1786.939 kb on - strand, within PS417_08160 at 1786.939 kb on - strand, within PS417_08160 at 1787.055 kb on + strand, within PS417_08160 at 1787.142 kb on + strand at 1787.142 kb on + strand at 1787.143 kb on - strand at 1787.143 kb on - strand at 1787.143 kb on - strand at 1787.163 kb on - strand at 1787.167 kb on + strand at 1787.168 kb on - strand at 1787.191 kb on + strand at 1787.192 kb on - strand at 1787.192 kb on - strand at 1787.208 kb on + strand at 1787.208 kb on + strand at 1787.219 kb on - strand at 1787.219 kb on - strand at 1787.259 kb on - strand at 1787.263 kb on + strand at 1787.287 kb on - strand at 1787.435 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.008 mg/ml remove 1,786,097 + -0.4 1,786,098 - +2.2 1,786,099 + -1.5 1,786,099 + +1.0 1,786,100 - +0.0 1,786,100 - -1.7 1,786,100 - +0.2 1,786,120 + -0.5 1,786,121 - +0.8 1,786,122 + -0.3 1,786,123 - +1.6 1,786,162 + +0.7 1,786,185 - +1.2 1,786,189 + +2.5 1,786,189 + +1.6 1,786,190 - +0.2 1,786,348 - PS417_08150 0.61 +0.8 1,786,396 - PS417_08150 0.78 +2.9 1,786,396 - PS417_08150 0.78 -0.8 1,786,426 + PS417_08150 0.89 +1.4 1,786,451 + +1.2 1,786,452 - +2.1 1,786,472 + -2.7 1,786,474 + +1.4 1,786,474 + +0.1 1,786,474 + +0.4 1,786,474 + +0.0 1,786,501 - +4.7 1,786,540 + +4.2 1,786,664 + PS417_08155 0.25 +4.7 1,786,673 + PS417_08155 0.28 +6.9 1,786,674 - PS417_08155 0.28 +8.6 1,786,906 + +2.7 1,786,938 + PS417_08160 0.13 +0.6 1,786,939 - PS417_08160 0.14 +3.2 1,786,939 - PS417_08160 0.14 +2.8 1,787,055 + PS417_08160 0.64 +1.1 1,787,142 + +2.3 1,787,142 + +0.6 1,787,143 - +3.3 1,787,143 - +3.3 1,787,143 - +3.0 1,787,163 - +2.7 1,787,167 + +0.7 1,787,168 - +5.1 1,787,191 + +0.8 1,787,192 - +1.2 1,787,192 - +2.2 1,787,208 + +2.5 1,787,208 + +0.6 1,787,219 - +3.4 1,787,219 - +2.8 1,787,259 - +3.4 1,787,263 + +0.7 1,787,287 - +2.9 1,787,435 - +4.6
Or see this region's nucleotide sequence