Experiment: Gentamicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05465 and PS417_05470 are separated by 55 nucleotides PS417_05470 and PS417_05475 are separated by 82 nucleotides PS417_05475 and PS417_05480 overlap by 4 nucleotides PS417_05480 and PS417_05485 overlap by 4 nucleotides PS417_05485 and PS417_05490 are separated by 80 nucleotides
PS417_05465: PS417_05465 - alpha/beta hydrolase, at 1,231,737 to 1,232,756
_05465
PS417_05470: PS417_05470 - DSBA oxidoreductase, at 1,232,812 to 1,233,465
_05470
PS417_05475: PS417_05475 - arginase, at 1,233,548 to 1,234,468
_05475
PS417_05480: PS417_05480 - transporter, at 1,234,465 to 1,234,845
_05480
PS417_05485: PS417_05485 - transporter, at 1,234,842 to 1,235,246
_05485
PS417_05490: PS417_05490 - fatty acid desaturase, at 1,235,327 to 1,236,199
_05490
Position (kb) 1233
1234
1235 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1232.591 kb on + strand, within PS417_05465 at 1232.682 kb on + strand at 1232.765 kb on + strand at 1232.765 kb on + strand at 1232.766 kb on - strand at 1232.766 kb on - strand at 1233.109 kb on + strand, within PS417_05470 at 1233.110 kb on - strand, within PS417_05470 at 1233.110 kb on - strand, within PS417_05470 at 1233.166 kb on + strand, within PS417_05470 at 1233.166 kb on + strand, within PS417_05470 at 1233.167 kb on - strand, within PS417_05470 at 1233.167 kb on - strand, within PS417_05470 at 1233.305 kb on - strand, within PS417_05470 at 1233.553 kb on + strand at 1233.767 kb on + strand, within PS417_05475 at 1234.195 kb on - strand, within PS417_05475 at 1234.308 kb on + strand, within PS417_05475 at 1234.308 kb on + strand, within PS417_05475 at 1234.309 kb on - strand, within PS417_05475 at 1234.309 kb on - strand, within PS417_05475 at 1235.285 kb on + strand at 1235.285 kb on + strand at 1235.285 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.008 mg/ml remove 1,232,591 + PS417_05465 0.84 -0.7 1,232,682 + +1.0 1,232,765 + -1.2 1,232,765 + +3.4 1,232,766 - -1.3 1,232,766 - +0.0 1,233,109 + PS417_05470 0.45 +0.6 1,233,110 - PS417_05470 0.46 +3.2 1,233,110 - PS417_05470 0.46 -2.1 1,233,166 + PS417_05470 0.54 +0.4 1,233,166 + PS417_05470 0.54 +0.4 1,233,167 - PS417_05470 0.54 -1.4 1,233,167 - PS417_05470 0.54 -0.0 1,233,305 - PS417_05470 0.75 -0.8 1,233,553 + -0.6 1,233,767 + PS417_05475 0.24 +0.7 1,234,195 - PS417_05475 0.70 +1.9 1,234,308 + PS417_05475 0.83 +1.9 1,234,308 + PS417_05475 0.83 +0.2 1,234,309 - PS417_05475 0.83 +1.6 1,234,309 - PS417_05475 0.83 -3.7 1,235,285 + +1.0 1,235,285 + +0.9 1,235,285 + -1.3
Or see this region's nucleotide sequence