Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0213 and Echvi_0214 are separated by 540 nucleotides Echvi_0214 and Echvi_0215 overlap by 17 nucleotides Echvi_0215 and Echvi_0216 overlap by 4 nucleotides
Echvi_0213: Echvi_0213 - hypothetical protein, at 233,060 to 233,374
_0213
Echvi_0214: Echvi_0214 - Putative transcription activator, at 233,915 to 234,568
_0214
Echvi_0215: Echvi_0215 - phosphomethylpyrimidine kinase, at 234,552 to 235,391
_0215
Echvi_0216: Echvi_0216 - thiamine-phosphate pyrophosphorylase, at 235,388 to 236,026
_0216
Position (kb) 233
234
235 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 232.918 kb on + strand at 232.919 kb on - strand at 232.919 kb on - strand at 233.050 kb on + strand at 233.050 kb on + strand at 233.050 kb on + strand at 233.050 kb on + strand at 233.076 kb on + strand at 233.079 kb on + strand at 233.080 kb on - strand at 233.102 kb on + strand, within Echvi_0213 at 233.103 kb on - strand, within Echvi_0213 at 233.103 kb on - strand, within Echvi_0213 at 233.106 kb on + strand, within Echvi_0213 at 233.106 kb on + strand, within Echvi_0213 at 233.119 kb on + strand, within Echvi_0213 at 233.136 kb on + strand, within Echvi_0213 at 233.136 kb on + strand, within Echvi_0213 at 233.136 kb on + strand, within Echvi_0213 at 233.137 kb on - strand, within Echvi_0213 at 233.155 kb on - strand, within Echvi_0213 at 233.178 kb on + strand, within Echvi_0213 at 233.263 kb on + strand, within Echvi_0213 at 233.373 kb on - strand at 233.582 kb on - strand at 233.643 kb on + strand at 233.643 kb on + strand at 233.653 kb on - strand at 233.716 kb on + strand at 233.734 kb on - strand at 233.818 kb on + strand at 233.843 kb on + strand at 234.091 kb on - strand, within Echvi_0214 at 234.091 kb on - strand, within Echvi_0214 at 234.094 kb on + strand, within Echvi_0214 at 234.094 kb on + strand, within Echvi_0214 at 234.095 kb on - strand, within Echvi_0214 at 234.095 kb on - strand, within Echvi_0214 at 234.095 kb on - strand, within Echvi_0214 at 234.141 kb on + strand, within Echvi_0214 at 234.142 kb on - strand, within Echvi_0214 at 234.142 kb on - strand, within Echvi_0214 at 234.145 kb on + strand, within Echvi_0214 at 234.146 kb on - strand, within Echvi_0214 at 234.163 kb on + strand, within Echvi_0214 at 234.163 kb on + strand, within Echvi_0214 at 234.207 kb on - strand, within Echvi_0214 at 234.207 kb on - strand, within Echvi_0214 at 234.237 kb on + strand, within Echvi_0214 at 234.237 kb on + strand, within Echvi_0214 at 234.237 kb on + strand, within Echvi_0214 at 234.237 kb on + strand, within Echvi_0214 at 234.237 kb on + strand, within Echvi_0214 at 234.238 kb on - strand, within Echvi_0214 at 234.238 kb on - strand, within Echvi_0214 at 234.238 kb on - strand, within Echvi_0214 at 234.330 kb on - strand, within Echvi_0214 at 234.457 kb on + strand, within Echvi_0214 at 234.593 kb on - strand at 234.593 kb on - strand at 234.883 kb on - strand, within Echvi_0215 at 234.883 kb on - strand, within Echvi_0215 at 235.143 kb on + strand, within Echvi_0215 at 235.224 kb on - strand, within Echvi_0215 at 235.224 kb on - strand, within Echvi_0215 at 235.230 kb on + strand, within Echvi_0215 at 235.241 kb on + strand, within Echvi_0215 at 235.253 kb on + strand, within Echvi_0215 at 235.253 kb on + strand, within Echvi_0215 at 235.254 kb on - strand, within Echvi_0215 at 235.369 kb on + strand at 235.369 kb on + strand at 235.369 kb on + strand at 235.382 kb on + strand at 235.522 kb on + strand, within Echvi_0216 at 235.523 kb on - strand, within Echvi_0216
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 232,918 + +0.9 232,919 - +0.5 232,919 - +1.5 233,050 + -0.7 233,050 + -0.2 233,050 + -1.8 233,050 + +1.1 233,076 + -1.7 233,079 + +1.2 233,080 - -1.4 233,102 + Echvi_0213 0.13 +1.5 233,103 - Echvi_0213 0.14 -0.8 233,103 - Echvi_0213 0.14 -0.2 233,106 + Echvi_0213 0.15 +0.6 233,106 + Echvi_0213 0.15 -0.9 233,119 + Echvi_0213 0.19 -0.1 233,136 + Echvi_0213 0.24 -2.1 233,136 + Echvi_0213 0.24 +0.4 233,136 + Echvi_0213 0.24 +1.3 233,137 - Echvi_0213 0.24 +0.1 233,155 - Echvi_0213 0.30 -0.2 233,178 + Echvi_0213 0.37 -0.9 233,263 + Echvi_0213 0.64 +0.4 233,373 - -0.4 233,582 - +0.7 233,643 + +0.8 233,643 + +0.2 233,653 - +1.1 233,716 + -2.9 233,734 - +0.2 233,818 + +0.5 233,843 + -1.3 234,091 - Echvi_0214 0.27 -0.1 234,091 - Echvi_0214 0.27 +0.3 234,094 + Echvi_0214 0.27 +1.4 234,094 + Echvi_0214 0.27 +0.9 234,095 - Echvi_0214 0.28 +0.5 234,095 - Echvi_0214 0.28 -0.5 234,095 - Echvi_0214 0.28 +1.9 234,141 + Echvi_0214 0.35 +0.7 234,142 - Echvi_0214 0.35 +1.4 234,142 - Echvi_0214 0.35 -1.7 234,145 + Echvi_0214 0.35 +0.4 234,146 - Echvi_0214 0.35 +1.4 234,163 + Echvi_0214 0.38 -0.2 234,163 + Echvi_0214 0.38 +1.1 234,207 - Echvi_0214 0.45 -2.1 234,207 - Echvi_0214 0.45 -1.9 234,237 + Echvi_0214 0.49 -0.6 234,237 + Echvi_0214 0.49 +0.8 234,237 + Echvi_0214 0.49 +0.9 234,237 + Echvi_0214 0.49 +0.7 234,237 + Echvi_0214 0.49 +3.1 234,238 - Echvi_0214 0.49 -1.1 234,238 - Echvi_0214 0.49 -0.4 234,238 - Echvi_0214 0.49 +0.3 234,330 - Echvi_0214 0.63 +1.4 234,457 + Echvi_0214 0.83 -0.2 234,593 - -3.0 234,593 - +1.3 234,883 - Echvi_0215 0.39 -0.2 234,883 - Echvi_0215 0.39 -3.0 235,143 + Echvi_0215 0.70 -1.8 235,224 - Echvi_0215 0.80 +0.2 235,224 - Echvi_0215 0.80 +0.1 235,230 + Echvi_0215 0.81 -1.8 235,241 + Echvi_0215 0.82 +0.5 235,253 + Echvi_0215 0.83 -0.3 235,253 + Echvi_0215 0.83 -0.9 235,254 - Echvi_0215 0.84 -0.4 235,369 + -1.3 235,369 + +0.9 235,369 + +0.2 235,382 + +1.1 235,522 + Echvi_0216 0.21 +0.7 235,523 - Echvi_0216 0.21 -2.4
Or see this region's nucleotide sequence