Experiment: Nalidixic 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04525 and PS417_04530 are separated by 30 nucleotides PS417_04530 and PS417_04535 are separated by 85 nucleotides PS417_04535 and PS417_04540 are separated by 258 nucleotides
PS417_04525: PS417_04525 - 16S rRNA methyltransferase, at 999,518 to 1,000,516
_04525
PS417_04530: PS417_04530 - glycerate dehydrogenase, at 1,000,547 to 1,001,512
_04530
PS417_04535: PS417_04535 - lysine transporter LysE, at 1,001,598 to 1,002,230
_04535
PS417_04540: PS417_04540 - long-chain fatty acid--CoA ligase, at 1,002,489 to 1,004,171
_04540
Position (kb)
1000
1001
1002 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 999.735 kb on - strand, within PS417_04525 at 999.854 kb on + strand, within PS417_04525 at 999.854 kb on + strand, within PS417_04525 at 999.855 kb on - strand, within PS417_04525 at 999.855 kb on - strand, within PS417_04525 at 999.855 kb on - strand, within PS417_04525 at 999.855 kb on - strand, within PS417_04525 at 999.855 kb on - strand, within PS417_04525 at 1000.371 kb on - strand, within PS417_04525 at 1000.496 kb on + strand at 1000.497 kb on - strand at 1000.497 kb on - strand at 1000.715 kb on + strand, within PS417_04530 at 1000.715 kb on + strand, within PS417_04530 at 1000.715 kb on + strand, within PS417_04530 at 1000.724 kb on + strand, within PS417_04530 at 1001.140 kb on - strand, within PS417_04530 at 1001.547 kb on - strand at 1001.547 kb on - strand at 1001.584 kb on + strand at 1001.584 kb on + strand at 1001.584 kb on + strand at 1001.585 kb on - strand at 1001.602 kb on - strand at 1001.638 kb on + strand at 1001.639 kb on - strand at 1001.850 kb on + strand, within PS417_04535 at 1001.851 kb on - strand, within PS417_04535 at 1001.851 kb on - strand, within PS417_04535 at 1002.202 kb on + strand at 1002.228 kb on + strand at 1002.378 kb on + strand at 1002.378 kb on + strand at 1002.430 kb on + strand at 1002.431 kb on - strand at 1002.431 kb on - strand at 1002.475 kb on + strand at 1002.476 kb on - strand at 1002.476 kb on - strand at 1002.488 kb on + strand at 1002.488 kb on + strand at 1002.489 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.025 mg/ml remove 999,735 - PS417_04525 0.22 +0.3 999,854 + PS417_04525 0.34 +1.0 999,854 + PS417_04525 0.34 -0.3 999,855 - PS417_04525 0.34 +0.5 999,855 - PS417_04525 0.34 -3.1 999,855 - PS417_04525 0.34 +0.1 999,855 - PS417_04525 0.34 +0.4 999,855 - PS417_04525 0.34 +0.5 1,000,371 - PS417_04525 0.85 +1.4 1,000,496 + +0.7 1,000,497 - +0.0 1,000,497 - +0.8 1,000,715 + PS417_04530 0.17 -0.1 1,000,715 + PS417_04530 0.17 +1.0 1,000,715 + PS417_04530 0.17 +0.1 1,000,724 + PS417_04530 0.18 +1.7 1,001,140 - PS417_04530 0.61 +1.4 1,001,547 - +0.5 1,001,547 - -0.0 1,001,584 + +1.7 1,001,584 + -0.1 1,001,584 + -1.2 1,001,585 - -0.1 1,001,602 - -2.4 1,001,638 + +0.5 1,001,639 - +0.2 1,001,850 + PS417_04535 0.40 -0.4 1,001,851 - PS417_04535 0.40 +1.1 1,001,851 - PS417_04535 0.40 +0.1 1,002,202 + -0.3 1,002,228 + +0.0 1,002,378 + -0.2 1,002,378 + -0.1 1,002,430 + +0.4 1,002,431 - +0.5 1,002,431 - +0.9 1,002,475 + +2.4 1,002,476 - +0.5 1,002,476 - +0.1 1,002,488 + +1.1 1,002,488 + +0.0 1,002,489 - +0.2
Or see this region's nucleotide sequence